In vitro evaluation of cytotoxic properties of 5-Aminolevulinic acid (5-ALA) on bladder cancer cells
Introduction: 5-Aminolevulinic (5-ALA) may be used as a photo diagnostic agent in bladder cancer. The aim of this study was to investigate the cytotoxic effect of 5-ALA on bladder cancer cells. Materials and methods: T24 cells treated with various concentrations of mitomycin (MC), 5-ALA and an MC/5...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/89332/ http://psasir.upm.edu.my/id/eprint/89332/1/CANCER.pdf |
| _version_ | 1848860828330098688 |
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| author | Fahmy Ahmed, Omar Ahmed Fahmy, Omar |
| author_facet | Fahmy Ahmed, Omar Ahmed Fahmy, Omar |
| author_sort | Fahmy Ahmed, Omar Ahmed |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Introduction: 5-Aminolevulinic (5-ALA) may be used as a photo diagnostic agent in bladder cancer. The aim of this study was to investigate the cytotoxic effect of 5-ALA on bladder cancer cells.
Materials and methods: T24 cells treated with various concentrations of mitomycin (MC), 5-ALA and an MC/5-ALA mixture were evaluated to determine the role of 5-ALA on MC cytotoxicity. Cell cycle analysis was conducted, and apoptosis was analyzed by flow cytometry. Caspase 3 enzyme and reactive oxygen species were measured. Results: Our initial studies exploring the impact of combination therapy on cell viability demonstrated improved cytotoxic effects on T24 and RT cells with relatively low doses of 5-ALA/MC in conjunction with MC alone. Indicated no significant difference between the IC50 of MC and MC/5-ALA in T24 cells, while IC50 value was decreased by 25 % in RT4 cells in 5-ALA/MC in comparing with MC alone. However, examination of cell cycle phase arrests by flow cytometry revealed significant PreG1 apoptosis and cell growth arrest in G2/M in T24 cells treated with the MC/5-ALA mixture compared with MC treatment. In addition, caspase 3 enzyme was increased by 1.15-fold in T24 cells treated with MC/5-ALA in comparison with MC alone. Conclusion: These results suggest that 5-ALA might possess anti-cancer properties and is not only a photo diagnostic element. |
| first_indexed | 2025-11-15T12:51:26Z |
| format | Article |
| id | upm-89332 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:51:26Z |
| publishDate | 2020 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-893322021-09-03T05:36:45Z http://psasir.upm.edu.my/id/eprint/89332/ In vitro evaluation of cytotoxic properties of 5-Aminolevulinic acid (5-ALA) on bladder cancer cells Fahmy Ahmed, Omar Ahmed Fahmy, Omar Introduction: 5-Aminolevulinic (5-ALA) may be used as a photo diagnostic agent in bladder cancer. The aim of this study was to investigate the cytotoxic effect of 5-ALA on bladder cancer cells. Materials and methods: T24 cells treated with various concentrations of mitomycin (MC), 5-ALA and an MC/5-ALA mixture were evaluated to determine the role of 5-ALA on MC cytotoxicity. Cell cycle analysis was conducted, and apoptosis was analyzed by flow cytometry. Caspase 3 enzyme and reactive oxygen species were measured. Results: Our initial studies exploring the impact of combination therapy on cell viability demonstrated improved cytotoxic effects on T24 and RT cells with relatively low doses of 5-ALA/MC in conjunction with MC alone. Indicated no significant difference between the IC50 of MC and MC/5-ALA in T24 cells, while IC50 value was decreased by 25 % in RT4 cells in 5-ALA/MC in comparing with MC alone. However, examination of cell cycle phase arrests by flow cytometry revealed significant PreG1 apoptosis and cell growth arrest in G2/M in T24 cells treated with the MC/5-ALA mixture compared with MC treatment. In addition, caspase 3 enzyme was increased by 1.15-fold in T24 cells treated with MC/5-ALA in comparison with MC alone. Conclusion: These results suggest that 5-ALA might possess anti-cancer properties and is not only a photo diagnostic element. Elsevier 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/89332/1/CANCER.pdf Fahmy Ahmed, Omar Ahmed and Fahmy, Omar (2020) In vitro evaluation of cytotoxic properties of 5-Aminolevulinic acid (5-ALA) on bladder cancer cells. Photodiagnosis and Photodynamic Therapy, 30. art. no. 101714. pp. 1-5. ISSN 1572-1000 https://www.sciencedirect.com/science/article/pii/S1572100020300673 10.1016/j.pdpdt.2020.101714 |
| spellingShingle | Fahmy Ahmed, Omar Ahmed Fahmy, Omar In vitro evaluation of cytotoxic properties of 5-Aminolevulinic acid (5-ALA) on bladder cancer cells |
| title | In vitro evaluation of cytotoxic properties of 5-Aminolevulinic acid (5-ALA) on bladder cancer cells |
| title_full | In vitro evaluation of cytotoxic properties of 5-Aminolevulinic acid (5-ALA) on bladder cancer cells |
| title_fullStr | In vitro evaluation of cytotoxic properties of 5-Aminolevulinic acid (5-ALA) on bladder cancer cells |
| title_full_unstemmed | In vitro evaluation of cytotoxic properties of 5-Aminolevulinic acid (5-ALA) on bladder cancer cells |
| title_short | In vitro evaluation of cytotoxic properties of 5-Aminolevulinic acid (5-ALA) on bladder cancer cells |
| title_sort | in vitro evaluation of cytotoxic properties of 5-aminolevulinic acid (5-ala) on bladder cancer cells |
| url | http://psasir.upm.edu.my/id/eprint/89332/ http://psasir.upm.edu.my/id/eprint/89332/ http://psasir.upm.edu.my/id/eprint/89332/ http://psasir.upm.edu.my/id/eprint/89332/1/CANCER.pdf |