In vitro evaluation of curcumin-encapsulated chitosan nanoparticles against feline infectious peritonitis virus and pharmacokinetics study in cats
Feline infectious peritonitis (FIP) is an important feline viral disease, causing an overridden inflammatory response that results in a high mortality rate, primarily in young cats. Curcumin is notable for its biological activities against various viral diseases; however, its poor bioavailability ha...
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| Format: | Article |
| Language: | English |
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Hindawi
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/89331/ http://psasir.upm.edu.my/id/eprint/89331/1/VITRO.pdf |
| _version_ | 1848860828024963072 |
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| author | Ng, Shing Wei Selvarajah, Gayathri Thevi Hussein, Mohd Zobir Yeap, Swee Keong Omar, Abdul Rahman |
| author_facet | Ng, Shing Wei Selvarajah, Gayathri Thevi Hussein, Mohd Zobir Yeap, Swee Keong Omar, Abdul Rahman |
| author_sort | Ng, Shing Wei |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Feline infectious peritonitis (FIP) is an important feline viral disease, causing an overridden inflammatory response that results in a high mortality rate, primarily in young cats. Curcumin is notable for its biological activities against various viral diseases; however, its poor bioavailability has hindered its potential in therapeutic application. In this study, curcumin was encapsulated in chitosan nanoparticles to improve its bioavailability. Curcumin-encapsulated chitosan (Cur-CS) nanoparticles were synthesised based on the ionic gelation technique and were spherical and cuboidal in shape, with an average particle size of 330 nm and +42 mV in zeta potential. The nanoparticles exerted lower toxicity in Crandell-Rees feline kidney (CrFK) cells and enhanced antiviral activities with a selective index (SI) value three times higher than that of curcumin. Feline-specific bead-based multiplex immunoassay and qPCR were used to examine their modulatory effects on proinflammatory cytokines, including tumour necrosis factor (TNF)α, interleukin- (IL-) 6, and IL-1β. There were significant decrements in IL-1β, IL-6, and TNFα expression in both curcumin and Cur-CS nanoparticles. Based on the multiplex immunoassay, curcumin and the Cur-CS nanoparticles could lower the immune-related proteins in FIP virus (FIPV) infection. The single- and multiple-dose pharmacokinetics profiles of curcumin and the Cur-CS nanoparticles were determined by high-performance liquid chromatography (HPLC). Oral delivery of the Cur-CS nanoparticles to cats showed enhanced bioavailability with a maximum plasma concentration () value of 621.5 ng/mL. Incorporating chitosan nanoparticles to deliver curcumin improved the oral bioavailability and antiviral effects of curcumin against FIPV infection. This study provides evidence for the potential of Cur-CS nanoparticles as a supplementary treatment of FIP. |
| first_indexed | 2025-11-15T12:51:26Z |
| format | Article |
| id | upm-89331 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:51:26Z |
| publishDate | 2020 |
| publisher | Hindawi |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-893312021-09-03T05:35:28Z http://psasir.upm.edu.my/id/eprint/89331/ In vitro evaluation of curcumin-encapsulated chitosan nanoparticles against feline infectious peritonitis virus and pharmacokinetics study in cats Ng, Shing Wei Selvarajah, Gayathri Thevi Hussein, Mohd Zobir Yeap, Swee Keong Omar, Abdul Rahman Feline infectious peritonitis (FIP) is an important feline viral disease, causing an overridden inflammatory response that results in a high mortality rate, primarily in young cats. Curcumin is notable for its biological activities against various viral diseases; however, its poor bioavailability has hindered its potential in therapeutic application. In this study, curcumin was encapsulated in chitosan nanoparticles to improve its bioavailability. Curcumin-encapsulated chitosan (Cur-CS) nanoparticles were synthesised based on the ionic gelation technique and were spherical and cuboidal in shape, with an average particle size of 330 nm and +42 mV in zeta potential. The nanoparticles exerted lower toxicity in Crandell-Rees feline kidney (CrFK) cells and enhanced antiviral activities with a selective index (SI) value three times higher than that of curcumin. Feline-specific bead-based multiplex immunoassay and qPCR were used to examine their modulatory effects on proinflammatory cytokines, including tumour necrosis factor (TNF)α, interleukin- (IL-) 6, and IL-1β. There were significant decrements in IL-1β, IL-6, and TNFα expression in both curcumin and Cur-CS nanoparticles. Based on the multiplex immunoassay, curcumin and the Cur-CS nanoparticles could lower the immune-related proteins in FIP virus (FIPV) infection. The single- and multiple-dose pharmacokinetics profiles of curcumin and the Cur-CS nanoparticles were determined by high-performance liquid chromatography (HPLC). Oral delivery of the Cur-CS nanoparticles to cats showed enhanced bioavailability with a maximum plasma concentration () value of 621.5 ng/mL. Incorporating chitosan nanoparticles to deliver curcumin improved the oral bioavailability and antiviral effects of curcumin against FIPV infection. This study provides evidence for the potential of Cur-CS nanoparticles as a supplementary treatment of FIP. Hindawi 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/89331/1/VITRO.pdf Ng, Shing Wei and Selvarajah, Gayathri Thevi and Hussein, Mohd Zobir and Yeap, Swee Keong and Omar, Abdul Rahman (2020) In vitro evaluation of curcumin-encapsulated chitosan nanoparticles against feline infectious peritonitis virus and pharmacokinetics study in cats. Biomed Research International, 2020. art. no. 3012198. pp. 1-18. ISSN 2314-6133; ESSN: 2314-6141 https://www.hindawi.com/journals/bmri/2020/3012198/ 10.1155/2020/3012198 |
| spellingShingle | Ng, Shing Wei Selvarajah, Gayathri Thevi Hussein, Mohd Zobir Yeap, Swee Keong Omar, Abdul Rahman In vitro evaluation of curcumin-encapsulated chitosan nanoparticles against feline infectious peritonitis virus and pharmacokinetics study in cats |
| title | In vitro evaluation of curcumin-encapsulated chitosan nanoparticles against feline infectious peritonitis virus and pharmacokinetics study in cats |
| title_full | In vitro evaluation of curcumin-encapsulated chitosan nanoparticles against feline infectious peritonitis virus and pharmacokinetics study in cats |
| title_fullStr | In vitro evaluation of curcumin-encapsulated chitosan nanoparticles against feline infectious peritonitis virus and pharmacokinetics study in cats |
| title_full_unstemmed | In vitro evaluation of curcumin-encapsulated chitosan nanoparticles against feline infectious peritonitis virus and pharmacokinetics study in cats |
| title_short | In vitro evaluation of curcumin-encapsulated chitosan nanoparticles against feline infectious peritonitis virus and pharmacokinetics study in cats |
| title_sort | in vitro evaluation of curcumin-encapsulated chitosan nanoparticles against feline infectious peritonitis virus and pharmacokinetics study in cats |
| url | http://psasir.upm.edu.my/id/eprint/89331/ http://psasir.upm.edu.my/id/eprint/89331/ http://psasir.upm.edu.my/id/eprint/89331/ http://psasir.upm.edu.my/id/eprint/89331/1/VITRO.pdf |