Identification of marine bacteria isolated from marine soil sediments and their ability to biosynthesise AgNPs extracellularly
Over the past few years, nanoparticles synthesis is one of the most active research in the nanotechnology field. The synthesis can be done chemically, physically and biologically. However, some researchers prefer to synthesise it biologically or also known as biosynthesis or ‘green synthesis’ becaus...
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| Format: | Article |
| Language: | English |
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Malaysian Society for Biochemistry and Molecular Biology
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/89255/ http://psasir.upm.edu.my/id/eprint/89255/1/ABSTRACT.pdf |
| _version_ | 1848860808135573504 |
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| author | Mohamad Noor, Aida Atiqah De Silva, Catrenar Gunasekaran, Baskaran Abd Gani, Shafinaz Abd Karim, Murni Marlina Ahmad, Siti Aqlima |
| author_facet | Mohamad Noor, Aida Atiqah De Silva, Catrenar Gunasekaran, Baskaran Abd Gani, Shafinaz Abd Karim, Murni Marlina Ahmad, Siti Aqlima |
| author_sort | Mohamad Noor, Aida Atiqah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Over the past few years, nanoparticles synthesis is one of the most active research in the nanotechnology field. The synthesis can be done chemically, physically and biologically. However, some researchers prefer to synthesise it biologically or also known as biosynthesis or ‘green synthesis’ because it is believed to be safer, environmentally friendly and cost-effective. In this work, we report the extracellular synthesis of 20 isolated marine bacteria from marine soil sediment which were identified and evaluated to synthesise silver nanoparticles (AgNPs). This was done by the addition of silver nitrate (AgNO3) solution with the cell-free supernatant of the isolated marine bacteria at room temperature. The marine bacteria were identified using 16S rRNA identification and neighbour-joining phylogenetic tree were constructed. Identification results showed that the isolated bacteria consist of 19 Serratia sp. and 1 Providencia sp. The biosynthesised AgNPs colloids were evaluated using morphological and optical analysis. AgNPs were observed for colour change and determined using ultraviolet-visible (UV-Vis) spectrophotometer. The existence of surface plasmon resonance peak at 400 to 450 nm is evidence of AgNPs formation. |
| first_indexed | 2025-11-15T12:51:07Z |
| format | Article |
| id | upm-89255 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:51:07Z |
| publishDate | 2020 |
| publisher | Malaysian Society for Biochemistry and Molecular Biology |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-892552021-10-07T03:54:57Z http://psasir.upm.edu.my/id/eprint/89255/ Identification of marine bacteria isolated from marine soil sediments and their ability to biosynthesise AgNPs extracellularly Mohamad Noor, Aida Atiqah De Silva, Catrenar Gunasekaran, Baskaran Abd Gani, Shafinaz Abd Karim, Murni Marlina Ahmad, Siti Aqlima Over the past few years, nanoparticles synthesis is one of the most active research in the nanotechnology field. The synthesis can be done chemically, physically and biologically. However, some researchers prefer to synthesise it biologically or also known as biosynthesis or ‘green synthesis’ because it is believed to be safer, environmentally friendly and cost-effective. In this work, we report the extracellular synthesis of 20 isolated marine bacteria from marine soil sediment which were identified and evaluated to synthesise silver nanoparticles (AgNPs). This was done by the addition of silver nitrate (AgNO3) solution with the cell-free supernatant of the isolated marine bacteria at room temperature. The marine bacteria were identified using 16S rRNA identification and neighbour-joining phylogenetic tree were constructed. Identification results showed that the isolated bacteria consist of 19 Serratia sp. and 1 Providencia sp. The biosynthesised AgNPs colloids were evaluated using morphological and optical analysis. AgNPs were observed for colour change and determined using ultraviolet-visible (UV-Vis) spectrophotometer. The existence of surface plasmon resonance peak at 400 to 450 nm is evidence of AgNPs formation. Malaysian Society for Biochemistry and Molecular Biology 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/89255/1/ABSTRACT.pdf Mohamad Noor, Aida Atiqah and De Silva, Catrenar and Gunasekaran, Baskaran and Abd Gani, Shafinaz and Abd Karim, Murni Marlina and Ahmad, Siti Aqlima (2020) Identification of marine bacteria isolated from marine soil sediments and their ability to biosynthesise AgNPs extracellularly. Malaysian Journal of Biochemistry and Molecular Biology, 23 (1). 52 - 56. ISSN 1511-2616 https://msbmb2010.wixsite.com/mjbmb/april-2020 |
| spellingShingle | Mohamad Noor, Aida Atiqah De Silva, Catrenar Gunasekaran, Baskaran Abd Gani, Shafinaz Abd Karim, Murni Marlina Ahmad, Siti Aqlima Identification of marine bacteria isolated from marine soil sediments and their ability to biosynthesise AgNPs extracellularly |
| title | Identification of marine bacteria isolated from marine soil sediments and their ability to biosynthesise AgNPs extracellularly |
| title_full | Identification of marine bacteria isolated from marine soil sediments and their ability to biosynthesise AgNPs extracellularly |
| title_fullStr | Identification of marine bacteria isolated from marine soil sediments and their ability to biosynthesise AgNPs extracellularly |
| title_full_unstemmed | Identification of marine bacteria isolated from marine soil sediments and their ability to biosynthesise AgNPs extracellularly |
| title_short | Identification of marine bacteria isolated from marine soil sediments and their ability to biosynthesise AgNPs extracellularly |
| title_sort | identification of marine bacteria isolated from marine soil sediments and their ability to biosynthesise agnps extracellularly |
| url | http://psasir.upm.edu.my/id/eprint/89255/ http://psasir.upm.edu.my/id/eprint/89255/ http://psasir.upm.edu.my/id/eprint/89255/1/ABSTRACT.pdf |