Methylmercury detection in maternal blood samples by liquid chromatography with inductively coupled plasma mass spectrometry
Methylmercury (MeHg) is one type of mercury (Hg) species known to be very toxic to humans, especially pregnant women and their fetuses. This study aims to obtain and validate the optimum condition of liquid chromatography with inductively coupled plasma mass spectrometry (LC-ICP-MS) to test MeHg con...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Universiti Putra Malaysia Press
2021
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| Online Access: | http://psasir.upm.edu.my/id/eprint/89249/ http://psasir.upm.edu.my/id/eprint/89249/1/PLASMA.pdf |
| _version_ | 1848860806400180224 |
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| author | Abedinlah, Amirah Mohd Elias, Saliza Praveena, Sarva Mangala Abu Bakar, Suhaili Rejali, Zulida Jalaludin, Juliana |
| author_facet | Abedinlah, Amirah Mohd Elias, Saliza Praveena, Sarva Mangala Abu Bakar, Suhaili Rejali, Zulida Jalaludin, Juliana |
| author_sort | Abedinlah, Amirah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Methylmercury (MeHg) is one type of mercury (Hg) species known to be very toxic to humans, especially pregnant women and their fetuses. This study aims to obtain and validate the optimum condition of liquid chromatography with inductively coupled plasma mass spectrometry (LC-ICP-MS) to test MeHg concentration. To date, there is limited research that is focused on the maternal blood MeHg samples using LC-ICPMS in Malaysia. Before analysis, collected blood (500 µL) was placed into a 15 mL polypropylene test tube, followed by the addition of extractant solution [0.10% (v/v)
HCl + 0.05% (m/v) L-cysteine + 0.10% (v/v) 2-mercaptoethanol] to the sample and sonicated for 15 minutes. The MeHg level was detected from the sample solution using the LC with Zorbax Eclipse XDB-C18 (4.6 x 12.5 mm, 5 µm) (Agilent Technologies) guard column and analytical column (4.6 x 150 mm, 5 µm) and was quantified by using the ICP-MS. The recovery of MeHg was in
the range of 106 to 112% with RSD of less than 10%, followed by the LOD and LOQ. |
| first_indexed | 2025-11-15T12:51:05Z |
| format | Article |
| id | upm-89249 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:51:05Z |
| publishDate | 2021 |
| publisher | Universiti Putra Malaysia Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-892492021-09-20T23:08:41Z http://psasir.upm.edu.my/id/eprint/89249/ Methylmercury detection in maternal blood samples by liquid chromatography with inductively coupled plasma mass spectrometry Abedinlah, Amirah Mohd Elias, Saliza Praveena, Sarva Mangala Abu Bakar, Suhaili Rejali, Zulida Jalaludin, Juliana Methylmercury (MeHg) is one type of mercury (Hg) species known to be very toxic to humans, especially pregnant women and their fetuses. This study aims to obtain and validate the optimum condition of liquid chromatography with inductively coupled plasma mass spectrometry (LC-ICP-MS) to test MeHg concentration. To date, there is limited research that is focused on the maternal blood MeHg samples using LC-ICPMS in Malaysia. Before analysis, collected blood (500 µL) was placed into a 15 mL polypropylene test tube, followed by the addition of extractant solution [0.10% (v/v) HCl + 0.05% (m/v) L-cysteine + 0.10% (v/v) 2-mercaptoethanol] to the sample and sonicated for 15 minutes. The MeHg level was detected from the sample solution using the LC with Zorbax Eclipse XDB-C18 (4.6 x 12.5 mm, 5 µm) (Agilent Technologies) guard column and analytical column (4.6 x 150 mm, 5 µm) and was quantified by using the ICP-MS. The recovery of MeHg was in the range of 106 to 112% with RSD of less than 10%, followed by the LOD and LOQ. Universiti Putra Malaysia Press 2021 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/89249/1/PLASMA.pdf Abedinlah, Amirah and Mohd Elias, Saliza and Praveena, Sarva Mangala and Abu Bakar, Suhaili and Rejali, Zulida and Jalaludin, Juliana (2021) Methylmercury detection in maternal blood samples by liquid chromatography with inductively coupled plasma mass spectrometry. Pertanika Journal of Science & Technology, 29 (3). pp. 2028-2044. ISSN 0128-7680; ESSN: 2231-8526 http://www.pertanika.upm.edu.my/pjst/browse/regular-issue?article=JST-2500-2021 10.47836/pjst.29.3.45 |
| spellingShingle | Abedinlah, Amirah Mohd Elias, Saliza Praveena, Sarva Mangala Abu Bakar, Suhaili Rejali, Zulida Jalaludin, Juliana Methylmercury detection in maternal blood samples by liquid chromatography with inductively coupled plasma mass spectrometry |
| title | Methylmercury detection in maternal blood samples by liquid chromatography with inductively coupled plasma mass spectrometry |
| title_full | Methylmercury detection in maternal blood samples by liquid chromatography with inductively coupled plasma mass spectrometry |
| title_fullStr | Methylmercury detection in maternal blood samples by liquid chromatography with inductively coupled plasma mass spectrometry |
| title_full_unstemmed | Methylmercury detection in maternal blood samples by liquid chromatography with inductively coupled plasma mass spectrometry |
| title_short | Methylmercury detection in maternal blood samples by liquid chromatography with inductively coupled plasma mass spectrometry |
| title_sort | methylmercury detection in maternal blood samples by liquid chromatography with inductively coupled plasma mass spectrometry |
| url | http://psasir.upm.edu.my/id/eprint/89249/ http://psasir.upm.edu.my/id/eprint/89249/ http://psasir.upm.edu.my/id/eprint/89249/ http://psasir.upm.edu.my/id/eprint/89249/1/PLASMA.pdf |