Growth promoting effects of Pluronic F-68 on callus proliferation of recalcitrant rice cultivar
This study was undertaken to evaluate growth-promoting effects of Pluronic F-68 (PF-68) on recalcitrant MR 219 rice callus. Our study shows that calli grown on Murashige and Skoog medium supplemented with 0.04% PF-68 significantly increased callus proliferation by 58.80% (fresh weight) and 23.98% (d...
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| Format: | Article |
| Language: | English |
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Springer Verlag
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/89139/ http://psasir.upm.edu.my/id/eprint/89139/1/CALLUS.pdf |
| _version_ | 1848860781886570496 |
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| author | Kok, Andrew De-Xian Wan Abdullah, Wan Muhamad Asrul Nizam Tan, Ngai Paing Abdullah, Janna Ong Sekeli, Rogayah Wee, Chien Yeong Lai, Kok Song |
| author_facet | Kok, Andrew De-Xian Wan Abdullah, Wan Muhamad Asrul Nizam Tan, Ngai Paing Abdullah, Janna Ong Sekeli, Rogayah Wee, Chien Yeong Lai, Kok Song |
| author_sort | Kok, Andrew De-Xian |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | This study was undertaken to evaluate growth-promoting effects of Pluronic F-68 (PF-68) on recalcitrant MR 219 rice callus. Our study shows that calli grown on Murashige and Skoog medium supplemented with 0.04% PF-68 significantly increased callus proliferation by 58.80% (fresh weight) and 23.98% (dry weight) while root formation from callus was enhanced by 28.57%. Enhanced callus proliferation was supported by biochemical analysis, whereby highest amount of soluble sugar (1.77 mg/mL) and protein (0.17 mg/mL) contents were recorded in calli grown on 0.04% PF-68. Furthermore, enhanced expression of sucrose synthase (2.65-folds) and NADH-dependent glutamate synthase (1.86-folds) genes in calli grown on 0.04% PF-68 also correlates with enhanced callus proliferation. In contrast, high concentration of PF-68 (0.10%) recorded highest amount of phenolic (0.74 mg/mL), flavonoid (0.08 mg/mL), and hydrogen peroxide content (0.06 mg/mL) as compared to other treatment groups indicates activation of plant defence mechanism towards stress. Similarly, high expression of 4-coumarate:CoA ligase 3 (1.28-folds), chalcone-flavonone isomerase (1.65-folds) and ascorbate peroxidase (1.61-folds) genes were observed in calli grown on 0.10% PF-68 further supports increasing stress caused by the high concentration of PF-68. Taken together, our study revealed that optimum concentration of PF-68 could improve recalcitrant rice callus proliferation via enhanced sugar metabolism and amino acid biosynthesis which are crucial towards plant growth and development. However, at high concentration, PF-68 induces stress in plant which enhance the production of secondary metabolite to maintain cellular homeostasis. |
| first_indexed | 2025-11-15T12:50:42Z |
| format | Article |
| id | upm-89139 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:50:42Z |
| publishDate | 2020 |
| publisher | Springer Verlag |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-891392021-09-04T00:02:29Z http://psasir.upm.edu.my/id/eprint/89139/ Growth promoting effects of Pluronic F-68 on callus proliferation of recalcitrant rice cultivar Kok, Andrew De-Xian Wan Abdullah, Wan Muhamad Asrul Nizam Tan, Ngai Paing Abdullah, Janna Ong Sekeli, Rogayah Wee, Chien Yeong Lai, Kok Song This study was undertaken to evaluate growth-promoting effects of Pluronic F-68 (PF-68) on recalcitrant MR 219 rice callus. Our study shows that calli grown on Murashige and Skoog medium supplemented with 0.04% PF-68 significantly increased callus proliferation by 58.80% (fresh weight) and 23.98% (dry weight) while root formation from callus was enhanced by 28.57%. Enhanced callus proliferation was supported by biochemical analysis, whereby highest amount of soluble sugar (1.77 mg/mL) and protein (0.17 mg/mL) contents were recorded in calli grown on 0.04% PF-68. Furthermore, enhanced expression of sucrose synthase (2.65-folds) and NADH-dependent glutamate synthase (1.86-folds) genes in calli grown on 0.04% PF-68 also correlates with enhanced callus proliferation. In contrast, high concentration of PF-68 (0.10%) recorded highest amount of phenolic (0.74 mg/mL), flavonoid (0.08 mg/mL), and hydrogen peroxide content (0.06 mg/mL) as compared to other treatment groups indicates activation of plant defence mechanism towards stress. Similarly, high expression of 4-coumarate:CoA ligase 3 (1.28-folds), chalcone-flavonone isomerase (1.65-folds) and ascorbate peroxidase (1.61-folds) genes were observed in calli grown on 0.10% PF-68 further supports increasing stress caused by the high concentration of PF-68. Taken together, our study revealed that optimum concentration of PF-68 could improve recalcitrant rice callus proliferation via enhanced sugar metabolism and amino acid biosynthesis which are crucial towards plant growth and development. However, at high concentration, PF-68 induces stress in plant which enhance the production of secondary metabolite to maintain cellular homeostasis. Springer Verlag 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/89139/1/CALLUS.pdf Kok, Andrew De-Xian and Wan Abdullah, Wan Muhamad Asrul Nizam and Tan, Ngai Paing and Abdullah, Janna Ong and Sekeli, Rogayah and Wee, Chien Yeong and Lai, Kok Song (2020) Growth promoting effects of Pluronic F-68 on callus proliferation of recalcitrant rice cultivar. 3 Biotech, 10 (3). pp. 1-10. ISSN 2190-572X; ESSN: 2190-5738 https://link.springer.com/article/10.1007%2Fs13205-020-2118-5 10.1007/s13205-020-2118-5 |
| spellingShingle | Kok, Andrew De-Xian Wan Abdullah, Wan Muhamad Asrul Nizam Tan, Ngai Paing Abdullah, Janna Ong Sekeli, Rogayah Wee, Chien Yeong Lai, Kok Song Growth promoting effects of Pluronic F-68 on callus proliferation of recalcitrant rice cultivar |
| title | Growth promoting effects of Pluronic F-68 on callus proliferation of recalcitrant rice cultivar |
| title_full | Growth promoting effects of Pluronic F-68 on callus proliferation of recalcitrant rice cultivar |
| title_fullStr | Growth promoting effects of Pluronic F-68 on callus proliferation of recalcitrant rice cultivar |
| title_full_unstemmed | Growth promoting effects of Pluronic F-68 on callus proliferation of recalcitrant rice cultivar |
| title_short | Growth promoting effects of Pluronic F-68 on callus proliferation of recalcitrant rice cultivar |
| title_sort | growth promoting effects of pluronic f-68 on callus proliferation of recalcitrant rice cultivar |
| url | http://psasir.upm.edu.my/id/eprint/89139/ http://psasir.upm.edu.my/id/eprint/89139/ http://psasir.upm.edu.my/id/eprint/89139/ http://psasir.upm.edu.my/id/eprint/89139/1/CALLUS.pdf |