Extraction, anti-tyrosinase, and antioxidant activities of the collagen hydrolysate derived from Rhopilema hispidum
The study was conducted to determine anti-tyrosinase and antioxidant activities of the extracted collagen hydrolysate (CH) derived from Malaysian jellyfish, Rhopilema hispidum. Collagen was extracted using 1:1 (w:v) 0.1 M NaOH solution at temperature 25 °C for 48 hr followed by treatment of 1:2 (w:v...
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| Format: | Article |
| Language: | English |
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Taylor and Francis
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/88943/ http://psasir.upm.edu.my/id/eprint/88943/1/ANTI.pdf |
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| author | Ab Aziz, Noor Atikah Salim, Norazlinaliza Zarei, Mohammad Saari, Nazamid Md. Yusoff, Fatimah |
| author_facet | Ab Aziz, Noor Atikah Salim, Norazlinaliza Zarei, Mohammad Saari, Nazamid Md. Yusoff, Fatimah |
| author_sort | Ab Aziz, Noor Atikah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The study was conducted to determine anti-tyrosinase and antioxidant activities of the extracted collagen hydrolysate (CH) derived from Malaysian jellyfish, Rhopilema hispidum. Collagen was extracted using 1:1 (w:v) 0.1 M NaOH solution at temperature 25 °C for 48 hr followed by treatment of 1:2 (w:v) distilled water for another 24 hr and freeze-dried. The extracted collagen was hydrolyzed using papain at optimum temperature, pH and enzyme/substrate ratio [E/S] of 60 °C, 7.0 and 1:50, respectively. CH was found to exhibit tyrosinase inhibitory activity, DPPH radical scavenging and metal ion-chelating assays up to 64, 28, and 83%, respectively, after 8 hr of hydrolysis process. The molecular weight of CH was found <10 kDa consisting of mainly Gly (19.219%), Glu (10.428%), and Arg (8.848%). The UV–visible spectrum analysis showed a major and minor peak at 218 and 276 nm, accordingly. The FTIR spectroscopy confirmed the amide groups in CH. The SEM images demonstrated spongy and porous structure of CH. In the cytotoxicity study, CH has no cytotoxicity against mouse embryonic 3T3 fibroblast cell line with IC50 value >500 µg/ml. Results revealed that the CH generated from this study has a potential to be developed as active ingredient in cosmeceutical application. |
| first_indexed | 2025-11-15T12:49:55Z |
| format | Article |
| id | upm-88943 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:49:55Z |
| publishDate | 2020 |
| publisher | Taylor and Francis |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-889432021-10-04T21:58:57Z http://psasir.upm.edu.my/id/eprint/88943/ Extraction, anti-tyrosinase, and antioxidant activities of the collagen hydrolysate derived from Rhopilema hispidum Ab Aziz, Noor Atikah Salim, Norazlinaliza Zarei, Mohammad Saari, Nazamid Md. Yusoff, Fatimah The study was conducted to determine anti-tyrosinase and antioxidant activities of the extracted collagen hydrolysate (CH) derived from Malaysian jellyfish, Rhopilema hispidum. Collagen was extracted using 1:1 (w:v) 0.1 M NaOH solution at temperature 25 °C for 48 hr followed by treatment of 1:2 (w:v) distilled water for another 24 hr and freeze-dried. The extracted collagen was hydrolyzed using papain at optimum temperature, pH and enzyme/substrate ratio [E/S] of 60 °C, 7.0 and 1:50, respectively. CH was found to exhibit tyrosinase inhibitory activity, DPPH radical scavenging and metal ion-chelating assays up to 64, 28, and 83%, respectively, after 8 hr of hydrolysis process. The molecular weight of CH was found <10 kDa consisting of mainly Gly (19.219%), Glu (10.428%), and Arg (8.848%). The UV–visible spectrum analysis showed a major and minor peak at 218 and 276 nm, accordingly. The FTIR spectroscopy confirmed the amide groups in CH. The SEM images demonstrated spongy and porous structure of CH. In the cytotoxicity study, CH has no cytotoxicity against mouse embryonic 3T3 fibroblast cell line with IC50 value >500 µg/ml. Results revealed that the CH generated from this study has a potential to be developed as active ingredient in cosmeceutical application. Taylor and Francis 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/88943/1/ANTI.pdf Ab Aziz, Noor Atikah and Salim, Norazlinaliza and Zarei, Mohammad and Saari, Nazamid and Md. Yusoff, Fatimah (2020) Extraction, anti-tyrosinase, and antioxidant activities of the collagen hydrolysate derived from Rhopilema hispidum. Preparative Biochemistry and Biotechnology, 51 (1). pp. 44-53. ISSN 1082-6068; ESSN: 1532-2297 https://www.tandfonline.com/doi/abs/10.1080/10826068.2020.1789991 10.1080/10826068.2020.1789991 |
| spellingShingle | Ab Aziz, Noor Atikah Salim, Norazlinaliza Zarei, Mohammad Saari, Nazamid Md. Yusoff, Fatimah Extraction, anti-tyrosinase, and antioxidant activities of the collagen hydrolysate derived from Rhopilema hispidum |
| title | Extraction, anti-tyrosinase, and antioxidant activities of the collagen hydrolysate derived from Rhopilema hispidum |
| title_full | Extraction, anti-tyrosinase, and antioxidant activities of the collagen hydrolysate derived from Rhopilema hispidum |
| title_fullStr | Extraction, anti-tyrosinase, and antioxidant activities of the collagen hydrolysate derived from Rhopilema hispidum |
| title_full_unstemmed | Extraction, anti-tyrosinase, and antioxidant activities of the collagen hydrolysate derived from Rhopilema hispidum |
| title_short | Extraction, anti-tyrosinase, and antioxidant activities of the collagen hydrolysate derived from Rhopilema hispidum |
| title_sort | extraction, anti-tyrosinase, and antioxidant activities of the collagen hydrolysate derived from rhopilema hispidum |
| url | http://psasir.upm.edu.my/id/eprint/88943/ http://psasir.upm.edu.my/id/eprint/88943/ http://psasir.upm.edu.my/id/eprint/88943/ http://psasir.upm.edu.my/id/eprint/88943/1/ANTI.pdf |