Electrochemical determination of zearalenone using a label-free competitive aptasensor
An electrochemical aptasensor is described for determination of the phytohormone of zearalenone (ZEA). The gold electrode was modified with ZEA via covalent attachment using cysteamine-hydrochloride and 1,4-phenylene diisocyanate linker. A truncated ZEA aptamer with a dissociation constant of 13.4...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Springer
2020
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/88771/ http://psasir.upm.edu.my/id/eprint/88771/1/WAVE.pdf |
| _version_ | 1848860692544749568 |
|---|---|
| author | Azri, Farah Asilah Eissa, Shimaa Zourob, Mohammed Chinnappan, Raja Sukor, Rashidah Yusof, Nor Azah Ahmad Raston, Nurul Hanun Alhoshani, Ali Selamat, Jinap |
| author_facet | Azri, Farah Asilah Eissa, Shimaa Zourob, Mohammed Chinnappan, Raja Sukor, Rashidah Yusof, Nor Azah Ahmad Raston, Nurul Hanun Alhoshani, Ali Selamat, Jinap |
| author_sort | Azri, Farah Asilah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | An electrochemical aptasensor is described for determination of the phytohormone of zearalenone (ZEA). The gold electrode was
modified with ZEA via covalent attachment using cysteamine-hydrochloride and 1,4-phenylene diisocyanate linker. A truncated
ZEA aptamer with a dissociation constant of 13.4 ± 2.1 nM was used in an aptasensor. The electrochemical property was
investigated using square wave voltammetry for monitoring the change in the electron transfer using the ferro/ferricyanide system as redox probe. Under optimal experimental conditions, the response was best measured at a potential of 0.20 V (vs. Ag/AgCl). The signals depended on the competitive mechanism between the immobilised ZEA and free ZEA for the aptamer binding site. The aptasensor works in the range 0.01 to 1000 ng·mL−1 ZEA concentration, with a detection limit of 0.017 ng· mL−1. High degree of cross-reactivity with the other analogues of ZEA was observed, whereas none towards other mycotoxins. The aptasensor was further applied for the determination of ZEA in the extract of maize grain and showed good recovery percentages between 87 and 110%. |
| first_indexed | 2025-11-15T12:49:17Z |
| format | Article |
| id | upm-88771 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:49:17Z |
| publishDate | 2020 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-887712021-10-06T15:40:57Z http://psasir.upm.edu.my/id/eprint/88771/ Electrochemical determination of zearalenone using a label-free competitive aptasensor Azri, Farah Asilah Eissa, Shimaa Zourob, Mohammed Chinnappan, Raja Sukor, Rashidah Yusof, Nor Azah Ahmad Raston, Nurul Hanun Alhoshani, Ali Selamat, Jinap An electrochemical aptasensor is described for determination of the phytohormone of zearalenone (ZEA). The gold electrode was modified with ZEA via covalent attachment using cysteamine-hydrochloride and 1,4-phenylene diisocyanate linker. A truncated ZEA aptamer with a dissociation constant of 13.4 ± 2.1 nM was used in an aptasensor. The electrochemical property was investigated using square wave voltammetry for monitoring the change in the electron transfer using the ferro/ferricyanide system as redox probe. Under optimal experimental conditions, the response was best measured at a potential of 0.20 V (vs. Ag/AgCl). The signals depended on the competitive mechanism between the immobilised ZEA and free ZEA for the aptamer binding site. The aptasensor works in the range 0.01 to 1000 ng·mL−1 ZEA concentration, with a detection limit of 0.017 ng· mL−1. High degree of cross-reactivity with the other analogues of ZEA was observed, whereas none towards other mycotoxins. The aptasensor was further applied for the determination of ZEA in the extract of maize grain and showed good recovery percentages between 87 and 110%. Springer 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/88771/1/WAVE.pdf Azri, Farah Asilah and Eissa, Shimaa and Zourob, Mohammed and Chinnappan, Raja and Sukor, Rashidah and Yusof, Nor Azah and Ahmad Raston, Nurul Hanun and Alhoshani, Ali and Selamat, Jinap (2020) Electrochemical determination of zearalenone using a label-free competitive aptasensor. Microchimica Acta, 187. art. no. 266. pp. 1-10. ISSN 0026-3672; ESSN: 1436-5073 https://link.springer.com/article/10.1007/s00604-020-4218-7 10.1007/s00604-020-4218-7 |
| spellingShingle | Azri, Farah Asilah Eissa, Shimaa Zourob, Mohammed Chinnappan, Raja Sukor, Rashidah Yusof, Nor Azah Ahmad Raston, Nurul Hanun Alhoshani, Ali Selamat, Jinap Electrochemical determination of zearalenone using a label-free competitive aptasensor |
| title | Electrochemical determination of zearalenone using a label-free competitive aptasensor |
| title_full | Electrochemical determination of zearalenone using a label-free competitive aptasensor |
| title_fullStr | Electrochemical determination of zearalenone using a label-free competitive aptasensor |
| title_full_unstemmed | Electrochemical determination of zearalenone using a label-free competitive aptasensor |
| title_short | Electrochemical determination of zearalenone using a label-free competitive aptasensor |
| title_sort | electrochemical determination of zearalenone using a label-free competitive aptasensor |
| url | http://psasir.upm.edu.my/id/eprint/88771/ http://psasir.upm.edu.my/id/eprint/88771/ http://psasir.upm.edu.my/id/eprint/88771/ http://psasir.upm.edu.my/id/eprint/88771/1/WAVE.pdf |