Effective refolding of a cysteine rich glycoside hydrolase family 19 recombinant chitinase from Streptomyces griseus by reverse dilution and affinity chromatography

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Effective refolding of a cysteine rich glycoside hydrolase family 19 recombinant chitinase from Streptomyces griseus by reverse dilution and affinity chromatography

Conventional refolding methods are associated with low yields due to misfolding and high aggregation rates or very dilute proteins. In this study, we describe the optimization of the conventional methods of reverse dilution and affinity chromatography for obtaining high yields of a cysteine rich rec...

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Bibliographic Details
Main Authors:	Oyeleye, Ayokunmi Omolola, Mohd Yusoff, Siti Faridah, Abd Rahim, Izzah Nadiah, Thean, Adam Chor Leow, Saidi, Noor Baity, Mohd Yahaya, Normi
Format:	Article
Language:	English
Published:	Public Library of Science 2020
Online Access:	http://psasir.upm.edu.my/id/eprint/88662/ http://psasir.upm.edu.my/id/eprint/88662/1/ABSTRACT.pdf

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