Effect of salt-tolerant bacterial inoculations on rice seedlings differing in salt-tolerance under saline soil conditions

Salt-tolerant plant growth-promoting rhizobacteria (PGPR) could be an alternative to alleviate salinity problems in rice plants grown in the coastal areas. This study was conducted to isolate and characterize salt-tolerant PGPR and observe their effects on the physiological and biochemical propertie...

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Main Authors: Shultana, Rakiba, Tan, Ali Kee Zuan, Yusop, Mohd Rafii, Mohd Saud, Halimi, Fatai, Arolu
Format: Article
Language:English
Published: Multidisciplinary Digital Publishing Institute 2020
Online Access:http://psasir.upm.edu.my/id/eprint/88634/
http://psasir.upm.edu.my/id/eprint/88634/1/ABSTRACT.pdf
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author Shultana, Rakiba
Tan, Ali Kee Zuan
Yusop, Mohd Rafii
Mohd Saud, Halimi
Fatai, Arolu
author_facet Shultana, Rakiba
Tan, Ali Kee Zuan
Yusop, Mohd Rafii
Mohd Saud, Halimi
Fatai, Arolu
author_sort Shultana, Rakiba
building UPM Institutional Repository
collection Online Access
description Salt-tolerant plant growth-promoting rhizobacteria (PGPR) could be an alternative to alleviate salinity problems in rice plants grown in the coastal areas. This study was conducted to isolate and characterize salt-tolerant PGPR and observe their effects on the physiological and biochemical properties of rice plants grown under non-saline and saline glasshouse conditions. Three strains were selected based on their salt-tolerance and plant growth-promoting properties under in vitro saline conditions. These strains were identified as Bacillus tequilensis (UPMRB9), Bacillus aryabhattai (UPMRE6), and Providencia stuartii (UPMRG1) using a 16S rRNA technique. The selected strains were inoculated to three different rice varieties, namely BRRI dhan67 (salt-tolerant), Putra-1 (moderate salt-tolerant), and MR297 (salt-susceptible) under glasshouse conditions. Results showed that the MR297 rice variety inoculated with UPMRB9 produced the highest total chlorophyll content, with an increment of 28%, and lowest electrolyte leakage of 92%. The Putra-1 rice variety also showed a 156% total dry matter increase with the inoculation of this bacterial strain. The highest increase of relative water content and reduction of Na/K ratio were found upon inoculation of UPMRE6 and UPMRB9, respectively. The biggest significant effects of these bacterial inoculations were on relative water content, electrolyte leakage, and the Na/K ratio of the BRRI dhan67 rice variety under saline conditions, suggesting a synergistic effect on the mechanisms of plant salt-tolerance. This study has shown that the application of locally-isolated salt-tolerant PGPR strains could be an effective long-term and sustainable solution for rice cultivation in the coastal areas, which are affected by global climate change.
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spelling upm-886342021-11-03T07:03:28Z http://psasir.upm.edu.my/id/eprint/88634/ Effect of salt-tolerant bacterial inoculations on rice seedlings differing in salt-tolerance under saline soil conditions Shultana, Rakiba Tan, Ali Kee Zuan Yusop, Mohd Rafii Mohd Saud, Halimi Fatai, Arolu Salt-tolerant plant growth-promoting rhizobacteria (PGPR) could be an alternative to alleviate salinity problems in rice plants grown in the coastal areas. This study was conducted to isolate and characterize salt-tolerant PGPR and observe their effects on the physiological and biochemical properties of rice plants grown under non-saline and saline glasshouse conditions. Three strains were selected based on their salt-tolerance and plant growth-promoting properties under in vitro saline conditions. These strains were identified as Bacillus tequilensis (UPMRB9), Bacillus aryabhattai (UPMRE6), and Providencia stuartii (UPMRG1) using a 16S rRNA technique. The selected strains were inoculated to three different rice varieties, namely BRRI dhan67 (salt-tolerant), Putra-1 (moderate salt-tolerant), and MR297 (salt-susceptible) under glasshouse conditions. Results showed that the MR297 rice variety inoculated with UPMRB9 produced the highest total chlorophyll content, with an increment of 28%, and lowest electrolyte leakage of 92%. The Putra-1 rice variety also showed a 156% total dry matter increase with the inoculation of this bacterial strain. The highest increase of relative water content and reduction of Na/K ratio were found upon inoculation of UPMRE6 and UPMRB9, respectively. The biggest significant effects of these bacterial inoculations were on relative water content, electrolyte leakage, and the Na/K ratio of the BRRI dhan67 rice variety under saline conditions, suggesting a synergistic effect on the mechanisms of plant salt-tolerance. This study has shown that the application of locally-isolated salt-tolerant PGPR strains could be an effective long-term and sustainable solution for rice cultivation in the coastal areas, which are affected by global climate change. Multidisciplinary Digital Publishing Institute 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/88634/1/ABSTRACT.pdf Shultana, Rakiba and Tan, Ali Kee Zuan and Yusop, Mohd Rafii and Mohd Saud, Halimi and Fatai, Arolu (2020) Effect of salt-tolerant bacterial inoculations on rice seedlings differing in salt-tolerance under saline soil conditions. Agronomy, 10 (7). pp. 1-22. ISSN 2073-4395 https://www.mdpi.com/2073-4395/10/7/1030 10.3390/agronomy10071030
spellingShingle Shultana, Rakiba
Tan, Ali Kee Zuan
Yusop, Mohd Rafii
Mohd Saud, Halimi
Fatai, Arolu
Effect of salt-tolerant bacterial inoculations on rice seedlings differing in salt-tolerance under saline soil conditions
title Effect of salt-tolerant bacterial inoculations on rice seedlings differing in salt-tolerance under saline soil conditions
title_full Effect of salt-tolerant bacterial inoculations on rice seedlings differing in salt-tolerance under saline soil conditions
title_fullStr Effect of salt-tolerant bacterial inoculations on rice seedlings differing in salt-tolerance under saline soil conditions
title_full_unstemmed Effect of salt-tolerant bacterial inoculations on rice seedlings differing in salt-tolerance under saline soil conditions
title_short Effect of salt-tolerant bacterial inoculations on rice seedlings differing in salt-tolerance under saline soil conditions
title_sort effect of salt-tolerant bacterial inoculations on rice seedlings differing in salt-tolerance under saline soil conditions
url http://psasir.upm.edu.my/id/eprint/88634/
http://psasir.upm.edu.my/id/eprint/88634/
http://psasir.upm.edu.my/id/eprint/88634/
http://psasir.upm.edu.my/id/eprint/88634/1/ABSTRACT.pdf