Effect of lignosulphonates on Vanilla planifolia shoot multiplication, regeneration and metabolism
Vanilla planifolia (V. planifolia) is a valuable orchidaceous plant, commonly grown for its pods that are used to produce the flavouring vanilla extract. Here, we evaluated the effect of calcium lignosulphonate (Ca-LIGN) and sodium lignosulphonate (Na-LIGN) on multiplication and regeneration of V. p...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Springer
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/88616/ http://psasir.upm.edu.my/id/eprint/88616/1/ABSTRACT.pdf |
| _version_ | 1848860657911332864 |
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| author | Wan Abdullah, Wan Muhamad Asrul Nizam Low, Lee Yoon Mohd Ramli Alzaki, Siti Bahijah Mumaiyizah Qian, Yee Chai Loh, Jiun Yan Abdullah, Janna Ong Lai, Kok Song |
| author_facet | Wan Abdullah, Wan Muhamad Asrul Nizam Low, Lee Yoon Mohd Ramli Alzaki, Siti Bahijah Mumaiyizah Qian, Yee Chai Loh, Jiun Yan Abdullah, Janna Ong Lai, Kok Song |
| author_sort | Wan Abdullah, Wan Muhamad Asrul Nizam |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Vanilla planifolia (V. planifolia) is a valuable orchidaceous plant, commonly grown for its pods that are used to produce the flavouring vanilla extract. Here, we evaluated the effect of calcium lignosulphonate (Ca-LIGN) and sodium lignosulphonate (Na-LIGN) on multiplication and regeneration of V. planifolia shoot tip culture. In 150 mg L−1 Ca-LIGN medium, the most number of shoots per explant (5.78 ± 0.63) was successfully obtained. Besides, Ca-LIGN also enhanced the shoot bud and primordial formation rate, as seen under scanning electron microscopy. In contrast, medium containing 150 mg L−1 Na-LIGN recorded the highest average of shoot length (4.72 ± 0.30 cm). Meanwhile, the best growth of root length (1.8 ± 0.32 cm) and root induction (96.67 ± 5.16%) were recorded on the explants treated with 150 mg L−1 Na-LIGN rooting medium. All rooted plantlets successfully acclimatized in the greenhouse (100.00% survival rate). Further biochemical analysis revealed that Ca-LIGN increased the total protein, chlorophyll, sugar, flavonoid and phenolic contents of V. planifolia. Notably, expression of both ribulose-1,5-bisphosphate carboxylase (Rubisco) and phosphoenolpyruvate carboxylase (PEPC) genes were also elevated under the treatment of Ca-LIGN, implying a positive role in the photosynthetic process. Taken together, LIGN being an environmental friendly product could be used to enhance the growth and micropropagation of V. planifolia. |
| first_indexed | 2025-11-15T12:48:44Z |
| format | Article |
| id | upm-88616 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:48:44Z |
| publishDate | 2020 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-886162021-12-14T23:47:15Z http://psasir.upm.edu.my/id/eprint/88616/ Effect of lignosulphonates on Vanilla planifolia shoot multiplication, regeneration and metabolism Wan Abdullah, Wan Muhamad Asrul Nizam Low, Lee Yoon Mohd Ramli Alzaki, Siti Bahijah Mumaiyizah Qian, Yee Chai Loh, Jiun Yan Abdullah, Janna Ong Lai, Kok Song Vanilla planifolia (V. planifolia) is a valuable orchidaceous plant, commonly grown for its pods that are used to produce the flavouring vanilla extract. Here, we evaluated the effect of calcium lignosulphonate (Ca-LIGN) and sodium lignosulphonate (Na-LIGN) on multiplication and regeneration of V. planifolia shoot tip culture. In 150 mg L−1 Ca-LIGN medium, the most number of shoots per explant (5.78 ± 0.63) was successfully obtained. Besides, Ca-LIGN also enhanced the shoot bud and primordial formation rate, as seen under scanning electron microscopy. In contrast, medium containing 150 mg L−1 Na-LIGN recorded the highest average of shoot length (4.72 ± 0.30 cm). Meanwhile, the best growth of root length (1.8 ± 0.32 cm) and root induction (96.67 ± 5.16%) were recorded on the explants treated with 150 mg L−1 Na-LIGN rooting medium. All rooted plantlets successfully acclimatized in the greenhouse (100.00% survival rate). Further biochemical analysis revealed that Ca-LIGN increased the total protein, chlorophyll, sugar, flavonoid and phenolic contents of V. planifolia. Notably, expression of both ribulose-1,5-bisphosphate carboxylase (Rubisco) and phosphoenolpyruvate carboxylase (PEPC) genes were also elevated under the treatment of Ca-LIGN, implying a positive role in the photosynthetic process. Taken together, LIGN being an environmental friendly product could be used to enhance the growth and micropropagation of V. planifolia. Springer 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/88616/1/ABSTRACT.pdf Wan Abdullah, Wan Muhamad Asrul Nizam and Low, Lee Yoon and Mohd Ramli Alzaki, Siti Bahijah Mumaiyizah and Qian, Yee Chai and Loh, Jiun Yan and Abdullah, Janna Ong and Lai, Kok Song (2020) Effect of lignosulphonates on Vanilla planifolia shoot multiplication, regeneration and metabolism. Acta Physiologiae Plantarum, 42. art. no. 107. pp. 1-8. ISSN 0137-5881; ESSN: 1861-1664 https://link.springer.com/article/10.1007/s11738-020-03099-9 10.1007/s11738-020-03099-9 |
| spellingShingle | Wan Abdullah, Wan Muhamad Asrul Nizam Low, Lee Yoon Mohd Ramli Alzaki, Siti Bahijah Mumaiyizah Qian, Yee Chai Loh, Jiun Yan Abdullah, Janna Ong Lai, Kok Song Effect of lignosulphonates on Vanilla planifolia shoot multiplication, regeneration and metabolism |
| title | Effect of lignosulphonates on Vanilla planifolia shoot multiplication, regeneration and metabolism |
| title_full | Effect of lignosulphonates on Vanilla planifolia shoot multiplication, regeneration and metabolism |
| title_fullStr | Effect of lignosulphonates on Vanilla planifolia shoot multiplication, regeneration and metabolism |
| title_full_unstemmed | Effect of lignosulphonates on Vanilla planifolia shoot multiplication, regeneration and metabolism |
| title_short | Effect of lignosulphonates on Vanilla planifolia shoot multiplication, regeneration and metabolism |
| title_sort | effect of lignosulphonates on vanilla planifolia shoot multiplication, regeneration and metabolism |
| url | http://psasir.upm.edu.my/id/eprint/88616/ http://psasir.upm.edu.my/id/eprint/88616/ http://psasir.upm.edu.my/id/eprint/88616/ http://psasir.upm.edu.my/id/eprint/88616/1/ABSTRACT.pdf |