Effect of honey as an additive for cryopreservation on bull semen quality from different cattle breeds under tropical condition
Previous studies have demonstrated that honey is a good additive for frozen semen, but the composition of honey is ultimately influenced by the plant species visited by the honey bees, the environment, processing, and storage conditions. Therefore, the main objective of this study was to determine t...
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| Format: | Article |
| Language: | English |
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Nexus Academic Publishers
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/88608/ http://psasir.upm.edu.my/id/eprint/88608/1/ABSTRACT.pdf |
| _version_ | 1848860655963078656 |
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| author | Lim, Eric Teik Chung Md Nayan, Mohd Nazri Mohammad Nasir, Nurul Syazana A. Hing, Phen Syom Ramli, Suriati Abdul Rahman, Mohd Hafiz Kamalludin, Mamat Hamidi |
| author_facet | Lim, Eric Teik Chung Md Nayan, Mohd Nazri Mohammad Nasir, Nurul Syazana A. Hing, Phen Syom Ramli, Suriati Abdul Rahman, Mohd Hafiz Kamalludin, Mamat Hamidi |
| author_sort | Lim, Eric Teik Chung |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Previous studies have demonstrated that honey is a good additive for frozen semen, but the composition of honey is ultimately influenced by the plant species visited by the honey bees, the environment, processing, and storage conditions. Therefore, the main objective of this study was to determine the effect of using local honey derived from the Apis mellifera bee as an additive in Bioxcell™ extender for cryopreservation on bull semen quality from different cattle breeds. Different concentrations of honey additive in the commercial extenders (1, 2.5, 5, 10 and 15%) were prepared accordingly. Semen samples were collected from 12 sexually mature bulls consisted of three Mafriwal, three Jersey, three Peidmontese, and three Limousin bulls with the aid of an artificial vagina (AV) and a teaser cow. The semen samples were extended 1:1 (semen: extender) in the prepared extenders for cryopreservation. Semen evaluations were carried out on fresh semen, post-chilled, and 14th days post-thawed frozen semen. The semen samples were evaluated based on microscopic characteristics such as general motility, progressive motility, and liveability. The results showed that there were significant differences (P˂0.05) in the sperm quality between different breeds of bull and between different concentrations of honey additive extenders. Jersey bull exhibited the best sperm quality with the highest sperm general motility, progressive motility, and live ability throughout the fresh semen, post-chilled, and post-thawed frozen semen followed by Mafriwal, Piedmontese, and Limousin. The sperm quality of all bull species increased significantly (P˂0.05) by the 1% concentration of honey additive extender compared to 2.5, 5, 10 and 15% concentration of honey additive extenders. In summary, 1% concentration of honey additive extender can be used effectively in bull semen cryopreservation to preserve the sperm quality. |
| first_indexed | 2025-11-15T12:48:42Z |
| format | Article |
| id | upm-88608 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:48:42Z |
| publishDate | 2020 |
| publisher | Nexus Academic Publishers |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-886082021-12-21T07:08:33Z http://psasir.upm.edu.my/id/eprint/88608/ Effect of honey as an additive for cryopreservation on bull semen quality from different cattle breeds under tropical condition Lim, Eric Teik Chung Md Nayan, Mohd Nazri Mohammad Nasir, Nurul Syazana A. Hing, Phen Syom Ramli, Suriati Abdul Rahman, Mohd Hafiz Kamalludin, Mamat Hamidi Previous studies have demonstrated that honey is a good additive for frozen semen, but the composition of honey is ultimately influenced by the plant species visited by the honey bees, the environment, processing, and storage conditions. Therefore, the main objective of this study was to determine the effect of using local honey derived from the Apis mellifera bee as an additive in Bioxcell™ extender for cryopreservation on bull semen quality from different cattle breeds. Different concentrations of honey additive in the commercial extenders (1, 2.5, 5, 10 and 15%) were prepared accordingly. Semen samples were collected from 12 sexually mature bulls consisted of three Mafriwal, three Jersey, three Peidmontese, and three Limousin bulls with the aid of an artificial vagina (AV) and a teaser cow. The semen samples were extended 1:1 (semen: extender) in the prepared extenders for cryopreservation. Semen evaluations were carried out on fresh semen, post-chilled, and 14th days post-thawed frozen semen. The semen samples were evaluated based on microscopic characteristics such as general motility, progressive motility, and liveability. The results showed that there were significant differences (P˂0.05) in the sperm quality between different breeds of bull and between different concentrations of honey additive extenders. Jersey bull exhibited the best sperm quality with the highest sperm general motility, progressive motility, and live ability throughout the fresh semen, post-chilled, and post-thawed frozen semen followed by Mafriwal, Piedmontese, and Limousin. The sperm quality of all bull species increased significantly (P˂0.05) by the 1% concentration of honey additive extender compared to 2.5, 5, 10 and 15% concentration of honey additive extenders. In summary, 1% concentration of honey additive extender can be used effectively in bull semen cryopreservation to preserve the sperm quality. Nexus Academic Publishers 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/88608/1/ABSTRACT.pdf Lim, Eric Teik Chung and Md Nayan, Mohd Nazri and Mohammad Nasir, Nurul Syazana and A. Hing, Phen Syom and Ramli, Suriati and Abdul Rahman, Mohd Hafiz and Kamalludin, Mamat Hamidi (2020) Effect of honey as an additive for cryopreservation on bull semen quality from different cattle breeds under tropical condition. Journal of Animal Health and Production, 7 (4). 171 - 178. ISSN 2308-2801 http://nexusacademicpublishers.com/table_contents_detail/11/1324/html 10.17582/journal.jahp/2019/7.4.171.178 |
| spellingShingle | Lim, Eric Teik Chung Md Nayan, Mohd Nazri Mohammad Nasir, Nurul Syazana A. Hing, Phen Syom Ramli, Suriati Abdul Rahman, Mohd Hafiz Kamalludin, Mamat Hamidi Effect of honey as an additive for cryopreservation on bull semen quality from different cattle breeds under tropical condition |
| title | Effect of honey as an additive for cryopreservation on bull semen quality from different cattle breeds under tropical condition |
| title_full | Effect of honey as an additive for cryopreservation on bull semen quality from different cattle breeds under tropical condition |
| title_fullStr | Effect of honey as an additive for cryopreservation on bull semen quality from different cattle breeds under tropical condition |
| title_full_unstemmed | Effect of honey as an additive for cryopreservation on bull semen quality from different cattle breeds under tropical condition |
| title_short | Effect of honey as an additive for cryopreservation on bull semen quality from different cattle breeds under tropical condition |
| title_sort | effect of honey as an additive for cryopreservation on bull semen quality from different cattle breeds under tropical condition |
| url | http://psasir.upm.edu.my/id/eprint/88608/ http://psasir.upm.edu.my/id/eprint/88608/ http://psasir.upm.edu.my/id/eprint/88608/ http://psasir.upm.edu.my/id/eprint/88608/1/ABSTRACT.pdf |