Effect of different cloning strategies in pET-28a on solubility and functionality of a staphylococcal phage endolysin

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Effect of different cloning strategies in pET-28a on solubility and functionality of a staphylococcal phage endolysin

Endolysins have been studied intensively as an alternative to antibiotics. In this study, endolysin derived from a phage which infects methicillin-resistant Staphylococcus aureus (MRSA) was cloned and expressed in Escherichia coli pET28a. Initially, the endolysin was cloned using BamHI/XhoI, resulti...

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Bibliographic Details
Main Authors:	Tham, Hong Y., Song, Adelene Ai Lian, Yusoff, Khatijah, Tan, Geok Hun
Format:	Article
Language:	English
Published:	Future Science 2020
Online Access:	http://psasir.upm.edu.my/id/eprint/88588/ http://psasir.upm.edu.my/id/eprint/88588/1/ABSTRACT.pdf

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