Clinacanthus nutans aqueous extract suppresses the release of histamine and β-Hexosaminidase inin-vitro model of IgE-mediated mast cell degranulation
Introduction: Clinacanthus nutans (Burm. f.) Lindau, a shrub found in South East Asia, particularly in Malaysia, Thailand, and Indonesia has many potential medicinal uses. It is used as a traditional herbal medicine for treating many diseases including skin rashes. Skin rash often appear in an aller...
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| Format: | Article |
| Language: | English |
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Medknow Publications
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/88171/ http://psasir.upm.edu.my/id/eprint/88171/1/ABSTRACT.pdf |
| _version_ | 1848860569584533504 |
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| author | Kow, Audrey Siew Foong Fong, Lai Yen Khoo, Leng Wei Tan, Ji Wei Lee, Ming Tatt Israf Ali, Daud Ahmad Abas, Faridah Shaari, Khozirah Tham, Chau Ling |
| author_facet | Kow, Audrey Siew Foong Fong, Lai Yen Khoo, Leng Wei Tan, Ji Wei Lee, Ming Tatt Israf Ali, Daud Ahmad Abas, Faridah Shaari, Khozirah Tham, Chau Ling |
| author_sort | Kow, Audrey Siew Foong |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Introduction: Clinacanthus nutans (Burm. f.) Lindau, a shrub found in South East Asia, particularly in Malaysia, Thailand, and Indonesia has many potential medicinal uses. It is used as a traditional herbal medicine for treating many diseases including skin rashes. Skin rash often appear in an allergic reaction. Recently, we have shown that C. nutans aqueous extract has the ability to alleviate ovalbumin-induced active systemic anaphylaxis rats from anaphylaxis – the acute form of allergy. This present study is aimed at comparing the ability of ethanolic and aqueous extracts of C. nutans to suppress IgE-mast cell degranulation. Materials and Methods: IgE-presensitized rat basophilic leukemic (RBL-2H3) cells pretreated with C. nutans ethanolic (100% ethanolic, 70% and 50% aqueous ethanolic) or 100% aqueous extracts were challenged with dinitrophenyl-bovine serum albumin to analyze the release of early and late-phase pro-inflammatory mediators. Results: We found that at concentrations of 5 mg/ml and above, C. nutans aqueous extract was able to suppress the levels of β-hexosaminidase and histamine while suppression was not seen in ethanolic extracts pretreated RBL-2H3 cells.Conclusion: Hence, we proposed that C. nutans aqueous extract is more active compared to the ethanolic extracts in suppressing the mediators of IgE-mast cell degranulation. |
| first_indexed | 2025-11-15T12:47:19Z |
| format | Article |
| id | upm-88171 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:47:19Z |
| publishDate | 2020 |
| publisher | Medknow Publications |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-881712022-05-18T02:40:45Z http://psasir.upm.edu.my/id/eprint/88171/ Clinacanthus nutans aqueous extract suppresses the release of histamine and β-Hexosaminidase inin-vitro model of IgE-mediated mast cell degranulation Kow, Audrey Siew Foong Fong, Lai Yen Khoo, Leng Wei Tan, Ji Wei Lee, Ming Tatt Israf Ali, Daud Ahmad Abas, Faridah Shaari, Khozirah Tham, Chau Ling Introduction: Clinacanthus nutans (Burm. f.) Lindau, a shrub found in South East Asia, particularly in Malaysia, Thailand, and Indonesia has many potential medicinal uses. It is used as a traditional herbal medicine for treating many diseases including skin rashes. Skin rash often appear in an allergic reaction. Recently, we have shown that C. nutans aqueous extract has the ability to alleviate ovalbumin-induced active systemic anaphylaxis rats from anaphylaxis – the acute form of allergy. This present study is aimed at comparing the ability of ethanolic and aqueous extracts of C. nutans to suppress IgE-mast cell degranulation. Materials and Methods: IgE-presensitized rat basophilic leukemic (RBL-2H3) cells pretreated with C. nutans ethanolic (100% ethanolic, 70% and 50% aqueous ethanolic) or 100% aqueous extracts were challenged with dinitrophenyl-bovine serum albumin to analyze the release of early and late-phase pro-inflammatory mediators. Results: We found that at concentrations of 5 mg/ml and above, C. nutans aqueous extract was able to suppress the levels of β-hexosaminidase and histamine while suppression was not seen in ethanolic extracts pretreated RBL-2H3 cells.Conclusion: Hence, we proposed that C. nutans aqueous extract is more active compared to the ethanolic extracts in suppressing the mediators of IgE-mast cell degranulation. Medknow Publications 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/88171/1/ABSTRACT.pdf Kow, Audrey Siew Foong and Fong, Lai Yen and Khoo, Leng Wei and Tan, Ji Wei and Lee, Ming Tatt and Israf Ali, Daud Ahmad and Abas, Faridah and Shaari, Khozirah and Tham, Chau Ling (2020) Clinacanthus nutans aqueous extract suppresses the release of histamine and β-Hexosaminidase inin-vitro model of IgE-mediated mast cell degranulation. Pharmacognosy Magazine, 16 (69). 400 - 408. ISSN 0973-1296; ESSN: 0976-4062 https://www.phcog.com/article.asp?issn=0973-1296;year=2020;volume=16;issue=69;spage=400;epage=408;aulast=Foong 10.4103/pm.pm_221_19 |
| spellingShingle | Kow, Audrey Siew Foong Fong, Lai Yen Khoo, Leng Wei Tan, Ji Wei Lee, Ming Tatt Israf Ali, Daud Ahmad Abas, Faridah Shaari, Khozirah Tham, Chau Ling Clinacanthus nutans aqueous extract suppresses the release of histamine and β-Hexosaminidase inin-vitro model of IgE-mediated mast cell degranulation |
| title | Clinacanthus nutans aqueous extract suppresses the release of histamine and β-Hexosaminidase inin-vitro model of IgE-mediated mast cell degranulation |
| title_full | Clinacanthus nutans aqueous extract suppresses the release of histamine and β-Hexosaminidase inin-vitro model of IgE-mediated mast cell degranulation |
| title_fullStr | Clinacanthus nutans aqueous extract suppresses the release of histamine and β-Hexosaminidase inin-vitro model of IgE-mediated mast cell degranulation |
| title_full_unstemmed | Clinacanthus nutans aqueous extract suppresses the release of histamine and β-Hexosaminidase inin-vitro model of IgE-mediated mast cell degranulation |
| title_short | Clinacanthus nutans aqueous extract suppresses the release of histamine and β-Hexosaminidase inin-vitro model of IgE-mediated mast cell degranulation |
| title_sort | clinacanthus nutans aqueous extract suppresses the release of histamine and β-hexosaminidase inin-vitro model of ige-mediated mast cell degranulation |
| url | http://psasir.upm.edu.my/id/eprint/88171/ http://psasir.upm.edu.my/id/eprint/88171/ http://psasir.upm.edu.my/id/eprint/88171/ http://psasir.upm.edu.my/id/eprint/88171/1/ABSTRACT.pdf |