Characterization on phenolic acids and antioxidant activity of Chlorella sp. microalgae using subcritical water extraction
Chlorella sp. microalgae contain phenolic compounds with good functional properties. In this study, two extraction methods, soxhlet and subcritical water extraction (SWE) were applied and compared in terms of phenolic compounds recovery from Chlorella sp. microalgae and characterization of the pheno...
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/88151/ http://psasir.upm.edu.my/id/eprint/88151/1/ABSTRACT.pdf |
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| author | Zakaria, Siti Maisurah Mustapa Kamal, Siti Mazlina Harun, Mohd Razif Omar, Rozita Siajam, Shamsul Izhar |
| author_facet | Zakaria, Siti Maisurah Mustapa Kamal, Siti Mazlina Harun, Mohd Razif Omar, Rozita Siajam, Shamsul Izhar |
| author_sort | Zakaria, Siti Maisurah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Chlorella sp. microalgae contain phenolic compounds with good functional properties. In this study, two extraction methods, soxhlet and subcritical water extraction (SWE) were applied and compared in terms of phenolic compounds recovery from Chlorella sp. microalgae and characterization of the phenolic acid components. Phenolic acid analysis demonstrated that the main components of the Chlorella sp. extracts were ferulic, caffeic and p-coumaric acids. The comparative study indicated that SWE gave higher extraction yield compared to conventional soxhlet method. High recoveries of phenolic acids were obtained at 175 °C with 3.20, 3.05 and 3.33 mg/100 g of ferulic, p-coumaric and caffeic acid, respectively, compared to soxhlet extraction using methanol with 2.10, 2.29 and 2.37 mg/100 g of ferulic, p-coumaric and caffeic acid, respectively. This proved that subcritical water treatment could effectively be used for the release of phenolic acids from Chlorella sp. using safe and green solvent. Analysis by Fourier transform infrared
spectroscopy (FTIR) was also performed to observe the effect of subcritical water on the functional groups of the extracts. The current study demonstrated that SWE provided a better way of utilising Chlorella sp. as a source of phenolic acids and natural antioxidants. |
| first_indexed | 2025-11-15T12:47:15Z |
| format | Article |
| id | upm-88151 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:47:15Z |
| publishDate | 2020 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-881512022-05-18T02:46:16Z http://psasir.upm.edu.my/id/eprint/88151/ Characterization on phenolic acids and antioxidant activity of Chlorella sp. microalgae using subcritical water extraction Zakaria, Siti Maisurah Mustapa Kamal, Siti Mazlina Harun, Mohd Razif Omar, Rozita Siajam, Shamsul Izhar Chlorella sp. microalgae contain phenolic compounds with good functional properties. In this study, two extraction methods, soxhlet and subcritical water extraction (SWE) were applied and compared in terms of phenolic compounds recovery from Chlorella sp. microalgae and characterization of the phenolic acid components. Phenolic acid analysis demonstrated that the main components of the Chlorella sp. extracts were ferulic, caffeic and p-coumaric acids. The comparative study indicated that SWE gave higher extraction yield compared to conventional soxhlet method. High recoveries of phenolic acids were obtained at 175 °C with 3.20, 3.05 and 3.33 mg/100 g of ferulic, p-coumaric and caffeic acid, respectively, compared to soxhlet extraction using methanol with 2.10, 2.29 and 2.37 mg/100 g of ferulic, p-coumaric and caffeic acid, respectively. This proved that subcritical water treatment could effectively be used for the release of phenolic acids from Chlorella sp. using safe and green solvent. Analysis by Fourier transform infrared spectroscopy (FTIR) was also performed to observe the effect of subcritical water on the functional groups of the extracts. The current study demonstrated that SWE provided a better way of utilising Chlorella sp. as a source of phenolic acids and natural antioxidants. Penerbit Universiti Kebangsaan Malaysia 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/88151/1/ABSTRACT.pdf Zakaria, Siti Maisurah and Mustapa Kamal, Siti Mazlina and Harun, Mohd Razif and Omar, Rozita and Siajam, Shamsul Izhar (2020) Characterization on phenolic acids and antioxidant activity of Chlorella sp. microalgae using subcritical water extraction. Sains Malaysiana, 49 (4). 765 - 774. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol49num4_2020/vol49num4_2020pg765-774.html 10.17576/jsm-2020-4904-05 |
| spellingShingle | Zakaria, Siti Maisurah Mustapa Kamal, Siti Mazlina Harun, Mohd Razif Omar, Rozita Siajam, Shamsul Izhar Characterization on phenolic acids and antioxidant activity of Chlorella sp. microalgae using subcritical water extraction |
| title | Characterization on phenolic acids and antioxidant activity of Chlorella sp. microalgae using subcritical water extraction |
| title_full | Characterization on phenolic acids and antioxidant activity of Chlorella sp. microalgae using subcritical water extraction |
| title_fullStr | Characterization on phenolic acids and antioxidant activity of Chlorella sp. microalgae using subcritical water extraction |
| title_full_unstemmed | Characterization on phenolic acids and antioxidant activity of Chlorella sp. microalgae using subcritical water extraction |
| title_short | Characterization on phenolic acids and antioxidant activity of Chlorella sp. microalgae using subcritical water extraction |
| title_sort | characterization on phenolic acids and antioxidant activity of chlorella sp. microalgae using subcritical water extraction |
| url | http://psasir.upm.edu.my/id/eprint/88151/ http://psasir.upm.edu.my/id/eprint/88151/ http://psasir.upm.edu.my/id/eprint/88151/ http://psasir.upm.edu.my/id/eprint/88151/1/ABSTRACT.pdf |