Characterization of Enterococcus hirae isolated from the intestine of Seabass (Lates Calcarifer) as a new potential probiotic against pathogenic vibrios
In this study, we aimed to isolate, identify and characterize lactic acid bacteria (LAB) from the intestine of juvenile seabass (Lates calcarifer) as a new potential probiotic. Four strains of LABs were isolated from the intestines of ten healthy seabass juveniles. In the in vitro screening process...
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| Format: | Article |
| Language: | English |
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Springer
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/88135/ http://psasir.upm.edu.my/id/eprint/88135/1/ABSTRACT.pdf |
| _version_ | 1848860561064853504 |
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| author | Masduki, Nur Fathiah Mohd Yaminudin, Nur Jasmin Chong, Chou Min Abd Karim, Murni Marlina |
| author_facet | Masduki, Nur Fathiah Mohd Yaminudin, Nur Jasmin Chong, Chou Min Abd Karim, Murni Marlina |
| author_sort | Masduki, Nur Fathiah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | In this study, we aimed to isolate, identify and characterize lactic acid bacteria (LAB) from the intestine of juvenile seabass (Lates calcarifer) as a new potential probiotic. Four strains of LABs were isolated from the intestines of ten healthy seabass juveniles. In the in vitro screening process using spot lawn assay, one isolate labeled as LAB3 showed inhibitory activity against Vibrio harveyi (ATCC 35,084). Strain LAB3 was determined to belong to the gram positive bacteria group with cocci shape and was identified as Enterococcus hirae using 16S rDNA analysis. This bacterium was able to grow at pH ranging from pH 2 to 10 with the best growth at pH 7. This strain was also able to grow at 0-4% NaCl after 24 h incubation and grew best at 1.5% NaCl. Enterococcus hirae strain LAB3 of the present study is worthy to be further characterized as a potential probiotic for use in seabass culture. |
| first_indexed | 2025-11-15T12:47:11Z |
| format | Article |
| id | upm-88135 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:47:11Z |
| publishDate | 2020 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-881352022-05-18T03:45:52Z http://psasir.upm.edu.my/id/eprint/88135/ Characterization of Enterococcus hirae isolated from the intestine of Seabass (Lates Calcarifer) as a new potential probiotic against pathogenic vibrios Masduki, Nur Fathiah Mohd Yaminudin, Nur Jasmin Chong, Chou Min Abd Karim, Murni Marlina In this study, we aimed to isolate, identify and characterize lactic acid bacteria (LAB) from the intestine of juvenile seabass (Lates calcarifer) as a new potential probiotic. Four strains of LABs were isolated from the intestines of ten healthy seabass juveniles. In the in vitro screening process using spot lawn assay, one isolate labeled as LAB3 showed inhibitory activity against Vibrio harveyi (ATCC 35,084). Strain LAB3 was determined to belong to the gram positive bacteria group with cocci shape and was identified as Enterococcus hirae using 16S rDNA analysis. This bacterium was able to grow at pH ranging from pH 2 to 10 with the best growth at pH 7. This strain was also able to grow at 0-4% NaCl after 24 h incubation and grew best at 1.5% NaCl. Enterococcus hirae strain LAB3 of the present study is worthy to be further characterized as a potential probiotic for use in seabass culture. Springer 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/88135/1/ABSTRACT.pdf Masduki, Nur Fathiah and Mohd Yaminudin, Nur Jasmin and Chong, Chou Min and Abd Karim, Murni Marlina (2020) Characterization of Enterococcus hirae isolated from the intestine of Seabass (Lates Calcarifer) as a new potential probiotic against pathogenic vibrios. Current Microbiology, 77 (12). pp. 1-7. ISSN 0343-8651; ESSN: 1432-0991 https://link.springer.com/article/10.1007/s00284-020-02228-4 10.1007/s00284-020-02228-4 |
| spellingShingle | Masduki, Nur Fathiah Mohd Yaminudin, Nur Jasmin Chong, Chou Min Abd Karim, Murni Marlina Characterization of Enterococcus hirae isolated from the intestine of Seabass (Lates Calcarifer) as a new potential probiotic against pathogenic vibrios |
| title | Characterization of Enterococcus hirae isolated from the intestine of Seabass (Lates Calcarifer) as a new potential probiotic against pathogenic vibrios |
| title_full | Characterization of Enterococcus hirae isolated from the intestine of Seabass (Lates Calcarifer) as a new potential probiotic against pathogenic vibrios |
| title_fullStr | Characterization of Enterococcus hirae isolated from the intestine of Seabass (Lates Calcarifer) as a new potential probiotic against pathogenic vibrios |
| title_full_unstemmed | Characterization of Enterococcus hirae isolated from the intestine of Seabass (Lates Calcarifer) as a new potential probiotic against pathogenic vibrios |
| title_short | Characterization of Enterococcus hirae isolated from the intestine of Seabass (Lates Calcarifer) as a new potential probiotic against pathogenic vibrios |
| title_sort | characterization of enterococcus hirae isolated from the intestine of seabass (lates calcarifer) as a new potential probiotic against pathogenic vibrios |
| url | http://psasir.upm.edu.my/id/eprint/88135/ http://psasir.upm.edu.my/id/eprint/88135/ http://psasir.upm.edu.my/id/eprint/88135/ http://psasir.upm.edu.my/id/eprint/88135/1/ABSTRACT.pdf |