Biosurfactant production and growth kinetics studies of the waste canola oil-degrading bacterium Rhodococcus erythropolis AQ5-07 from Antarctica
With the progressive increase in human activities in the Antarctic region, the possibility of domestic oil spillage also increases. Developing means for the removal of oils, such as canola oil, from the environment and waste "grey" water using biological approaches is therefore desirable,...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Multidisciplinary Digital Publishing Institute
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/88043/ http://psasir.upm.edu.my/id/eprint/88043/1/ABSTRACT.pdf |
| _version_ | 1848860544172294144 |
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| author | Ibrahim, Salihu Abdul Khalil, Khalilah Mohd Zahri, Khadijah Nabilah Gomez-Fuentes, Claudio Convey, Peter Zulkharnain, Azham Sabri, Suriana Alias, Siti Aisyah González-Rocha, Gerardo Ahmad, Siti Aqlima |
| author_facet | Ibrahim, Salihu Abdul Khalil, Khalilah Mohd Zahri, Khadijah Nabilah Gomez-Fuentes, Claudio Convey, Peter Zulkharnain, Azham Sabri, Suriana Alias, Siti Aisyah González-Rocha, Gerardo Ahmad, Siti Aqlima |
| author_sort | Ibrahim, Salihu |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | With the progressive increase in human activities in the Antarctic region, the possibility of domestic oil spillage also increases. Developing means for the removal of oils, such as canola oil, from the environment and waste "grey" water using biological approaches is therefore desirable, since the thermal process of oil degradation is expensive and ineffective. Thus, in this study an indigenous cold-adapted Antarctic soil bacterium, Rhodococcus erythropolis strain AQ5-07, was screened for biosurfactant production ability using the multiple approaches of blood haemolysis, surface tension, emulsification index, oil spreading, drop collapse and "MATH" assay for cellular hydrophobicity. The growth kinetics of the bacterium containing different canola oil concentration was studied. The strain showed β-haemolysis on blood agar with a high emulsification index and low surface tension value of 91.5% and 25.14 mN/m, respectively. Of the models tested, the Haldane model provided the best description of the growth kinetics, although several models were similar in performance. Parameters obtained from the modelling were the maximum specific growth rate (qmax), concentration of substrate at the half maximum specific growth rate, Ks% (v/v) and the inhibition constant Ki% (v/v), with values of 0.142 h-1, 7.743% (v/v) and 0.399% (v/v), respectively. These biological coefficients are useful in predicting growth conditions for batch studies, and also relevant to "in field" bioremediation strategies where the concentration of oil might need to be diluted to non-toxic levels prior to remediation. Biosurfactants can also have application in enhanced oil recovery (EOR) under different environmental conditions. |
| first_indexed | 2025-11-15T12:46:55Z |
| format | Article |
| id | upm-88043 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:46:55Z |
| publishDate | 2020 |
| publisher | Multidisciplinary Digital Publishing Institute |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-880432022-05-24T03:58:53Z http://psasir.upm.edu.my/id/eprint/88043/ Biosurfactant production and growth kinetics studies of the waste canola oil-degrading bacterium Rhodococcus erythropolis AQ5-07 from Antarctica Ibrahim, Salihu Abdul Khalil, Khalilah Mohd Zahri, Khadijah Nabilah Gomez-Fuentes, Claudio Convey, Peter Zulkharnain, Azham Sabri, Suriana Alias, Siti Aisyah González-Rocha, Gerardo Ahmad, Siti Aqlima With the progressive increase in human activities in the Antarctic region, the possibility of domestic oil spillage also increases. Developing means for the removal of oils, such as canola oil, from the environment and waste "grey" water using biological approaches is therefore desirable, since the thermal process of oil degradation is expensive and ineffective. Thus, in this study an indigenous cold-adapted Antarctic soil bacterium, Rhodococcus erythropolis strain AQ5-07, was screened for biosurfactant production ability using the multiple approaches of blood haemolysis, surface tension, emulsification index, oil spreading, drop collapse and "MATH" assay for cellular hydrophobicity. The growth kinetics of the bacterium containing different canola oil concentration was studied. The strain showed β-haemolysis on blood agar with a high emulsification index and low surface tension value of 91.5% and 25.14 mN/m, respectively. Of the models tested, the Haldane model provided the best description of the growth kinetics, although several models were similar in performance. Parameters obtained from the modelling were the maximum specific growth rate (qmax), concentration of substrate at the half maximum specific growth rate, Ks% (v/v) and the inhibition constant Ki% (v/v), with values of 0.142 h-1, 7.743% (v/v) and 0.399% (v/v), respectively. These biological coefficients are useful in predicting growth conditions for batch studies, and also relevant to "in field" bioremediation strategies where the concentration of oil might need to be diluted to non-toxic levels prior to remediation. Biosurfactants can also have application in enhanced oil recovery (EOR) under different environmental conditions. Multidisciplinary Digital Publishing Institute 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/88043/1/ABSTRACT.pdf Ibrahim, Salihu and Abdul Khalil, Khalilah and Mohd Zahri, Khadijah Nabilah and Gomez-Fuentes, Claudio and Convey, Peter and Zulkharnain, Azham and Sabri, Suriana and Alias, Siti Aisyah and González-Rocha, Gerardo and Ahmad, Siti Aqlima (2020) Biosurfactant production and growth kinetics studies of the waste canola oil-degrading bacterium Rhodococcus erythropolis AQ5-07 from Antarctica. Molecules, 25 (17). pp. 1-15. ISSN 1420-3049 https://www.mdpi.com/1420-3049/25/17/3878 10.3390/molecules25173878 |
| spellingShingle | Ibrahim, Salihu Abdul Khalil, Khalilah Mohd Zahri, Khadijah Nabilah Gomez-Fuentes, Claudio Convey, Peter Zulkharnain, Azham Sabri, Suriana Alias, Siti Aisyah González-Rocha, Gerardo Ahmad, Siti Aqlima Biosurfactant production and growth kinetics studies of the waste canola oil-degrading bacterium Rhodococcus erythropolis AQ5-07 from Antarctica |
| title | Biosurfactant production and growth kinetics studies of the waste canola oil-degrading bacterium Rhodococcus erythropolis AQ5-07 from Antarctica |
| title_full | Biosurfactant production and growth kinetics studies of the waste canola oil-degrading bacterium Rhodococcus erythropolis AQ5-07 from Antarctica |
| title_fullStr | Biosurfactant production and growth kinetics studies of the waste canola oil-degrading bacterium Rhodococcus erythropolis AQ5-07 from Antarctica |
| title_full_unstemmed | Biosurfactant production and growth kinetics studies of the waste canola oil-degrading bacterium Rhodococcus erythropolis AQ5-07 from Antarctica |
| title_short | Biosurfactant production and growth kinetics studies of the waste canola oil-degrading bacterium Rhodococcus erythropolis AQ5-07 from Antarctica |
| title_sort | biosurfactant production and growth kinetics studies of the waste canola oil-degrading bacterium rhodococcus erythropolis aq5-07 from antarctica |
| url | http://psasir.upm.edu.my/id/eprint/88043/ http://psasir.upm.edu.my/id/eprint/88043/ http://psasir.upm.edu.my/id/eprint/88043/ http://psasir.upm.edu.my/id/eprint/88043/1/ABSTRACT.pdf |