Biocompatible nutmeg oil-loaded nanoemulsion as phyto-repellent

Plant essential oils are widely used in perfumes and insect repellent products. However, due to the high volatility of the constituents in essential oils, their efficacy as a repellent product is less effective than that of synthetic compounds. Using a nanoemulsion as a carrier is one way to overcom...

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Main Authors: Mohd Narawi, Masturah, Hock, Ing Chiu, Yong, Yoke Keong, Mohamad Zain, Nur Nadhirah, Ramachandran, Muggundha Raoov, Tham, Chau Ling, Samsurrijal, Siti Fatimah, Lim, Vuanghao
Format: Article
Language:English
Published: Frontiers Media 2020
Online Access:http://psasir.upm.edu.my/id/eprint/88026/
http://psasir.upm.edu.my/id/eprint/88026/1/ABSTRACT.pdf
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author Mohd Narawi, Masturah
Hock, Ing Chiu
Yong, Yoke Keong
Mohamad Zain, Nur Nadhirah
Ramachandran, Muggundha Raoov
Tham, Chau Ling
Samsurrijal, Siti Fatimah
Lim, Vuanghao
author_facet Mohd Narawi, Masturah
Hock, Ing Chiu
Yong, Yoke Keong
Mohamad Zain, Nur Nadhirah
Ramachandran, Muggundha Raoov
Tham, Chau Ling
Samsurrijal, Siti Fatimah
Lim, Vuanghao
author_sort Mohd Narawi, Masturah
building UPM Institutional Repository
collection Online Access
description Plant essential oils are widely used in perfumes and insect repellent products. However, due to the high volatility of the constituents in essential oils, their efficacy as a repellent product is less effective than that of synthetic compounds. Using a nanoemulsion as a carrier is one way to overcome this disadvantage of essential oils. Nutmeg oil-loaded nanoemulsion (NT) was prepared using a high speed homogenizer and sonicator with varying amounts of surfactant, glycerol, and distilled water. Using a phase diagram, different formulations were tested for their droplet size and insect repellent activity. The nanoemulsion containing 6.25% surfactant and 91.25% glycerol (NT 6) had the highest percentage of protection (87.81%) in terms of repellent activity among the formulations tested for the 8 h duration of the experiment. The droplet size of NT 6 was 217.4 nm, and its polydispersity index (PDI) was 0.248. The zeta potential value was -44.2 mV, and the viscosity was 2.49 Pa.s at pH 5.6. The in vitro release profile was 71.5%. When the cytotoxicity of NT 6 at 400 μg/mL was tested using the MTS assay, cell viability was 97.38%. Physical appearance and stability of the nanoemulsion improved with the addition of glycerol as a co-solvent. In summary, a nutmeg oil-loaded nanoemulsion was successfully formulated and its controlled release of the essential oil showed mosquito repellent activity, thus eliminating the disadvantages of essential oils.
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spelling upm-880262022-05-24T04:48:56Z http://psasir.upm.edu.my/id/eprint/88026/ Biocompatible nutmeg oil-loaded nanoemulsion as phyto-repellent Mohd Narawi, Masturah Hock, Ing Chiu Yong, Yoke Keong Mohamad Zain, Nur Nadhirah Ramachandran, Muggundha Raoov Tham, Chau Ling Samsurrijal, Siti Fatimah Lim, Vuanghao Plant essential oils are widely used in perfumes and insect repellent products. However, due to the high volatility of the constituents in essential oils, their efficacy as a repellent product is less effective than that of synthetic compounds. Using a nanoemulsion as a carrier is one way to overcome this disadvantage of essential oils. Nutmeg oil-loaded nanoemulsion (NT) was prepared using a high speed homogenizer and sonicator with varying amounts of surfactant, glycerol, and distilled water. Using a phase diagram, different formulations were tested for their droplet size and insect repellent activity. The nanoemulsion containing 6.25% surfactant and 91.25% glycerol (NT 6) had the highest percentage of protection (87.81%) in terms of repellent activity among the formulations tested for the 8 h duration of the experiment. The droplet size of NT 6 was 217.4 nm, and its polydispersity index (PDI) was 0.248. The zeta potential value was -44.2 mV, and the viscosity was 2.49 Pa.s at pH 5.6. The in vitro release profile was 71.5%. When the cytotoxicity of NT 6 at 400 μg/mL was tested using the MTS assay, cell viability was 97.38%. Physical appearance and stability of the nanoemulsion improved with the addition of glycerol as a co-solvent. In summary, a nutmeg oil-loaded nanoemulsion was successfully formulated and its controlled release of the essential oil showed mosquito repellent activity, thus eliminating the disadvantages of essential oils. Frontiers Media 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/88026/1/ABSTRACT.pdf Mohd Narawi, Masturah and Hock, Ing Chiu and Yong, Yoke Keong and Mohamad Zain, Nur Nadhirah and Ramachandran, Muggundha Raoov and Tham, Chau Ling and Samsurrijal, Siti Fatimah and Lim, Vuanghao (2020) Biocompatible nutmeg oil-loaded nanoemulsion as phyto-repellent. Frontiers in Pharmacology, 11. art. no. 214. pp. 1-15. ISSN 1663-9812 https://www.frontiersin.org/articles/10.3389/fphar.2020.00214/full 10.3389/fphar.2020.00214
spellingShingle Mohd Narawi, Masturah
Hock, Ing Chiu
Yong, Yoke Keong
Mohamad Zain, Nur Nadhirah
Ramachandran, Muggundha Raoov
Tham, Chau Ling
Samsurrijal, Siti Fatimah
Lim, Vuanghao
Biocompatible nutmeg oil-loaded nanoemulsion as phyto-repellent
title Biocompatible nutmeg oil-loaded nanoemulsion as phyto-repellent
title_full Biocompatible nutmeg oil-loaded nanoemulsion as phyto-repellent
title_fullStr Biocompatible nutmeg oil-loaded nanoemulsion as phyto-repellent
title_full_unstemmed Biocompatible nutmeg oil-loaded nanoemulsion as phyto-repellent
title_short Biocompatible nutmeg oil-loaded nanoemulsion as phyto-repellent
title_sort biocompatible nutmeg oil-loaded nanoemulsion as phyto-repellent
url http://psasir.upm.edu.my/id/eprint/88026/
http://psasir.upm.edu.my/id/eprint/88026/
http://psasir.upm.edu.my/id/eprint/88026/
http://psasir.upm.edu.my/id/eprint/88026/1/ABSTRACT.pdf