Antibacterial activity of nutmeg (Myristica fragrans Houtt.) extract against foodborne pathogens on raw beef during chilled and frozen storage
In recent years, demands for minimal processing and free-synthetic preservatives are increasing because of growing concern among consumers regarding the safety issues of additives. Nutmeg (Myristica fragrans Houtt.) has been used as a spice and traditional medicine in Asian countries. This study ai...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Rynnye Lyan Resources
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/87884/ http://psasir.upm.edu.my/id/eprint/87884/1/ABSTRACT.pdf |
| _version_ | 1848860520500690944 |
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| author | Lagha, Osama Mohamed Ben Zakaria, Mohamad Pauzi Ismail, Intan Safinar Mahmud @ Ab Rashid, Nor Khaizura Rukayadi, Yaya |
| author_facet | Lagha, Osama Mohamed Ben Zakaria, Mohamad Pauzi Ismail, Intan Safinar Mahmud @ Ab Rashid, Nor Khaizura Rukayadi, Yaya |
| author_sort | Lagha, Osama Mohamed Ben |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | In recent years, demands for minimal processing and free-synthetic preservatives are increasing because of growing concern among consumers regarding the safety issues of additives. Nutmeg (Myristica fragrans Houtt.) has been used as a spice and traditional
medicine in Asian countries. This study aimed to determine the antibacterial activity of nutmeg extract against foodborne pathogens on raw beef during storage. Nutmeg seeds were extracted using a maceration method with methanol as a solvent. The extract was
assessed for antibacterial activity against a range of microorganisms using the disc diffusion assay, minimum inhibitory concentration and minimum bactericidal concentration. The effect of heat and different pH of the extract on its antibacterial activity was also conducted to evaluate the stability of the extract. The effects of the extract at
different concentrations on the microbial population of the raw beef during chilled (4.0±0.2oC) and frozen (-18.0±0.2oC) storage for 21 days were then evaluated. The nutmeg extract exhibited antimicrobial activity against the range of microorganisms tested, which was stable at high temperature (80.0±2.0oC) and in the pH range tested (3.0, 6.8, and 11). Furthermore, the extract significantly inhibited microbial growth on raw beef stored for 21 days in chilled or frozen conditions, indicating that the nutmeg extract has the potential to be developed as a natural antimicrobial preservative in beef. |
| first_indexed | 2025-11-15T12:46:32Z |
| format | Article |
| id | upm-87884 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:46:32Z |
| publishDate | 2020 |
| publisher | Rynnye Lyan Resources |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-878842022-06-07T06:47:33Z http://psasir.upm.edu.my/id/eprint/87884/ Antibacterial activity of nutmeg (Myristica fragrans Houtt.) extract against foodborne pathogens on raw beef during chilled and frozen storage Lagha, Osama Mohamed Ben Zakaria, Mohamad Pauzi Ismail, Intan Safinar Mahmud @ Ab Rashid, Nor Khaizura Rukayadi, Yaya In recent years, demands for minimal processing and free-synthetic preservatives are increasing because of growing concern among consumers regarding the safety issues of additives. Nutmeg (Myristica fragrans Houtt.) has been used as a spice and traditional medicine in Asian countries. This study aimed to determine the antibacterial activity of nutmeg extract against foodborne pathogens on raw beef during storage. Nutmeg seeds were extracted using a maceration method with methanol as a solvent. The extract was assessed for antibacterial activity against a range of microorganisms using the disc diffusion assay, minimum inhibitory concentration and minimum bactericidal concentration. The effect of heat and different pH of the extract on its antibacterial activity was also conducted to evaluate the stability of the extract. The effects of the extract at different concentrations on the microbial population of the raw beef during chilled (4.0±0.2oC) and frozen (-18.0±0.2oC) storage for 21 days were then evaluated. The nutmeg extract exhibited antimicrobial activity against the range of microorganisms tested, which was stable at high temperature (80.0±2.0oC) and in the pH range tested (3.0, 6.8, and 11). Furthermore, the extract significantly inhibited microbial growth on raw beef stored for 21 days in chilled or frozen conditions, indicating that the nutmeg extract has the potential to be developed as a natural antimicrobial preservative in beef. Rynnye Lyan Resources 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/87884/1/ABSTRACT.pdf Lagha, Osama Mohamed Ben and Zakaria, Mohamad Pauzi and Ismail, Intan Safinar and Mahmud @ Ab Rashid, Nor Khaizura and Rukayadi, Yaya (2020) Antibacterial activity of nutmeg (Myristica fragrans Houtt.) extract against foodborne pathogens on raw beef during chilled and frozen storage. Food Research, 4 (2). 380 - 388. ISSN 2550-2166 https://www.myfoodresearch.com/uploads/8/4/8/5/84855864/_16__fr-2019-280_ben_lagha_3.pdf 10.26656/fr.2017.4(2).280 |
| spellingShingle | Lagha, Osama Mohamed Ben Zakaria, Mohamad Pauzi Ismail, Intan Safinar Mahmud @ Ab Rashid, Nor Khaizura Rukayadi, Yaya Antibacterial activity of nutmeg (Myristica fragrans Houtt.) extract against foodborne pathogens on raw beef during chilled and frozen storage |
| title | Antibacterial activity of nutmeg (Myristica fragrans Houtt.) extract against foodborne pathogens on raw beef during chilled and frozen storage |
| title_full | Antibacterial activity of nutmeg (Myristica fragrans Houtt.) extract against foodborne pathogens on raw beef during chilled and frozen storage |
| title_fullStr | Antibacterial activity of nutmeg (Myristica fragrans Houtt.) extract against foodborne pathogens on raw beef during chilled and frozen storage |
| title_full_unstemmed | Antibacterial activity of nutmeg (Myristica fragrans Houtt.) extract against foodborne pathogens on raw beef during chilled and frozen storage |
| title_short | Antibacterial activity of nutmeg (Myristica fragrans Houtt.) extract against foodborne pathogens on raw beef during chilled and frozen storage |
| title_sort | antibacterial activity of nutmeg (myristica fragrans houtt.) extract against foodborne pathogens on raw beef during chilled and frozen storage |
| url | http://psasir.upm.edu.my/id/eprint/87884/ http://psasir.upm.edu.my/id/eprint/87884/ http://psasir.upm.edu.my/id/eprint/87884/ http://psasir.upm.edu.my/id/eprint/87884/1/ABSTRACT.pdf |