Analysis of expression of vitamin E-binding proteins in H2O2 induced SK-N-SH neuronal cells supplemented with α-tocopherol and tocotrienol-rich fraction
Natural α-tocopherol (α-TCP), but not tocotrienol, is preferentially retained in the human body. α-Tocopherol transfer protein (α-TTP) is responsible for binding α-TCP for cellular uptake and has high affinity and specificity for α-TCP but not α-tocotrienol. The purpose of this study was to examine...
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Public Library of Science
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/87857/ http://psasir.upm.edu.my/id/eprint/87857/1/ABSTRACT.pdf |
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| author | Chiroma, Aishatu Ali Khaza’ai, Huzwah Abd. Hamid@ Abd. Razak, Roslida Chang, Sui Kiat Zakaria, Zainul Amiruddin Zainal, Zaida |
| author_facet | Chiroma, Aishatu Ali Khaza’ai, Huzwah Abd. Hamid@ Abd. Razak, Roslida Chang, Sui Kiat Zakaria, Zainul Amiruddin Zainal, Zaida |
| author_sort | Chiroma, Aishatu Ali |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Natural α-tocopherol (α-TCP), but not tocotrienol, is preferentially retained in the human body. α-Tocopherol transfer protein (α-TTP) is responsible for binding α-TCP for cellular uptake and has high affinity and specificity for α-TCP but not α-tocotrienol. The purpose of this study was to examine the modification of α-TTP together with other related vitamin E-binding genes (i.e., TTPA, SEC14L2, and PI-TPNA) in regulating vitamin E uptake in neuronal cells at rest and under oxidative stress. Oxidative stress was induced with H2O2 for an hour which was followed by supplementation with different ratios of α-TCP and tocotrienol-rich fraction (TRF) for four hours. The cellular levels of vitamin E were quantified to determine bioavailability at cellular levels. The expression levels of TTPA, SEC14L2, and PI-TPNA genes in 0% α-TCP were found to be positively correlated with the levels of vitamin E in resting neuronal cells. In addition, the regulation of all the above-mentioned genes affect the distribution of vitamin E in the neuronal cells. It was observed that, increased levels of α-TCP secretion occur under oxidative stress. Thus, our results showed that in conclusion vitamin E-binding proteins may be modified in the absence of α-TCP to produce tocotrienols (TCT), as a source of vitamin E. The current study suggests that the expression levels of vitamin E transport proteins may influence the cellular concentrations of vitamin E levels in the neuronal cells. |
| first_indexed | 2025-11-15T12:46:26Z |
| format | Article |
| id | upm-87857 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:46:26Z |
| publishDate | 2020 |
| publisher | Public Library of Science |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-878572022-05-27T23:39:33Z http://psasir.upm.edu.my/id/eprint/87857/ Analysis of expression of vitamin E-binding proteins in H2O2 induced SK-N-SH neuronal cells supplemented with α-tocopherol and tocotrienol-rich fraction Chiroma, Aishatu Ali Khaza’ai, Huzwah Abd. Hamid@ Abd. Razak, Roslida Chang, Sui Kiat Zakaria, Zainul Amiruddin Zainal, Zaida Natural α-tocopherol (α-TCP), but not tocotrienol, is preferentially retained in the human body. α-Tocopherol transfer protein (α-TTP) is responsible for binding α-TCP for cellular uptake and has high affinity and specificity for α-TCP but not α-tocotrienol. The purpose of this study was to examine the modification of α-TTP together with other related vitamin E-binding genes (i.e., TTPA, SEC14L2, and PI-TPNA) in regulating vitamin E uptake in neuronal cells at rest and under oxidative stress. Oxidative stress was induced with H2O2 for an hour which was followed by supplementation with different ratios of α-TCP and tocotrienol-rich fraction (TRF) for four hours. The cellular levels of vitamin E were quantified to determine bioavailability at cellular levels. The expression levels of TTPA, SEC14L2, and PI-TPNA genes in 0% α-TCP were found to be positively correlated with the levels of vitamin E in resting neuronal cells. In addition, the regulation of all the above-mentioned genes affect the distribution of vitamin E in the neuronal cells. It was observed that, increased levels of α-TCP secretion occur under oxidative stress. Thus, our results showed that in conclusion vitamin E-binding proteins may be modified in the absence of α-TCP to produce tocotrienols (TCT), as a source of vitamin E. The current study suggests that the expression levels of vitamin E transport proteins may influence the cellular concentrations of vitamin E levels in the neuronal cells. Public Library of Science 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/87857/1/ABSTRACT.pdf Chiroma, Aishatu Ali and Khaza’ai, Huzwah and Abd. Hamid@ Abd. Razak, Roslida and Chang, Sui Kiat and Zakaria, Zainul Amiruddin and Zainal, Zaida (2020) Analysis of expression of vitamin E-binding proteins in H2O2 induced SK-N-SH neuronal cells supplemented with α-tocopherol and tocotrienol-rich fraction. PLoS One, 15 (11). art. no. 0241112. pp. 1-18. ISSN 1932-6203 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0241112 10.1371/journal.pone.0241112 |
| spellingShingle | Chiroma, Aishatu Ali Khaza’ai, Huzwah Abd. Hamid@ Abd. Razak, Roslida Chang, Sui Kiat Zakaria, Zainul Amiruddin Zainal, Zaida Analysis of expression of vitamin E-binding proteins in H2O2 induced SK-N-SH neuronal cells supplemented with α-tocopherol and tocotrienol-rich fraction |
| title | Analysis of expression of vitamin E-binding proteins in H2O2 induced SK-N-SH neuronal cells supplemented with α-tocopherol and tocotrienol-rich fraction |
| title_full | Analysis of expression of vitamin E-binding proteins in H2O2 induced SK-N-SH neuronal cells supplemented with α-tocopherol and tocotrienol-rich fraction |
| title_fullStr | Analysis of expression of vitamin E-binding proteins in H2O2 induced SK-N-SH neuronal cells supplemented with α-tocopherol and tocotrienol-rich fraction |
| title_full_unstemmed | Analysis of expression of vitamin E-binding proteins in H2O2 induced SK-N-SH neuronal cells supplemented with α-tocopherol and tocotrienol-rich fraction |
| title_short | Analysis of expression of vitamin E-binding proteins in H2O2 induced SK-N-SH neuronal cells supplemented with α-tocopherol and tocotrienol-rich fraction |
| title_sort | analysis of expression of vitamin e-binding proteins in h2o2 induced sk-n-sh neuronal cells supplemented with α-tocopherol and tocotrienol-rich fraction |
| url | http://psasir.upm.edu.my/id/eprint/87857/ http://psasir.upm.edu.my/id/eprint/87857/ http://psasir.upm.edu.my/id/eprint/87857/ http://psasir.upm.edu.my/id/eprint/87857/1/ABSTRACT.pdf |