An electrochemical sensor based on gold nanoparticles-functionalized reduced graphene oxide screen printed electrode for the detection of pyocyanin biomarker in Pseudomonas aeruginosa infection
Multidrug resistant Pseudomonas aeruginosa (P. aeruginosa) is known to be a problematic bacterium for being a major cause of opportunistic and nosocomial infections. In this study, reduced graphene oxide decorated with gold nanoparticles (AuNPs/rGO) was utilized as a new sensing material for a fast...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/87822/ http://psasir.upm.edu.my/id/eprint/87822/1/ABSTRACT.pdf |
| _version_ | 1848860507290730496 |
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| author | Abdul Rashid, Jahwarhar Izuan Kannan, Vayithiswary Ahmad, Muhammad Hafiz Mon, Aye Aye Taufik, Safura Miskon, Azizi Ong, Keat Khim Yusof, Nor Azah |
| author_facet | Abdul Rashid, Jahwarhar Izuan Kannan, Vayithiswary Ahmad, Muhammad Hafiz Mon, Aye Aye Taufik, Safura Miskon, Azizi Ong, Keat Khim Yusof, Nor Azah |
| author_sort | Abdul Rashid, Jahwarhar Izuan |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Multidrug resistant Pseudomonas aeruginosa (P. aeruginosa) is known to be a problematic bacterium for being a major cause of opportunistic and nosocomial infections. In this study, reduced graphene oxide decorated with gold nanoparticles (AuNPs/rGO) was utilized as a new sensing material for a fast and direct electrochemical detection of pyocyanin as a biomarker of P. aeruginosa infections. Under optimal condition, the developed electrochemical pyocyanin sensor exhibited a good linear range for the determination of pyocyanin in phosphate-buffered saline (PBS), human saliva and urine at a clinically relevant concentration range of 1–100 μM, achieving a detection limit of 0.27 μM, 1.34 μM, and 2.3 μM, respectively. Our developed sensor demonstrated good selectivity towards pyocyanin in the presence of interfering molecule such as ascorbic acid, uric acid, NADH, glucose, and acetylsalicylic acid, which are commonly found in human fluids. Furthermore, the developed sensor was able to discriminate the signal with and without the presence of pyocyanin directly in P. aeruginosa culture. This proposed technique demonstrates its potential application in monitoring the presence of P. aeruginosa infection in patients. |
| first_indexed | 2025-11-15T12:46:20Z |
| format | Article |
| id | upm-87822 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:46:20Z |
| publishDate | 2020 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-878222022-06-07T06:54:12Z http://psasir.upm.edu.my/id/eprint/87822/ An electrochemical sensor based on gold nanoparticles-functionalized reduced graphene oxide screen printed electrode for the detection of pyocyanin biomarker in Pseudomonas aeruginosa infection Abdul Rashid, Jahwarhar Izuan Kannan, Vayithiswary Ahmad, Muhammad Hafiz Mon, Aye Aye Taufik, Safura Miskon, Azizi Ong, Keat Khim Yusof, Nor Azah Multidrug resistant Pseudomonas aeruginosa (P. aeruginosa) is known to be a problematic bacterium for being a major cause of opportunistic and nosocomial infections. In this study, reduced graphene oxide decorated with gold nanoparticles (AuNPs/rGO) was utilized as a new sensing material for a fast and direct electrochemical detection of pyocyanin as a biomarker of P. aeruginosa infections. Under optimal condition, the developed electrochemical pyocyanin sensor exhibited a good linear range for the determination of pyocyanin in phosphate-buffered saline (PBS), human saliva and urine at a clinically relevant concentration range of 1–100 μM, achieving a detection limit of 0.27 μM, 1.34 μM, and 2.3 μM, respectively. Our developed sensor demonstrated good selectivity towards pyocyanin in the presence of interfering molecule such as ascorbic acid, uric acid, NADH, glucose, and acetylsalicylic acid, which are commonly found in human fluids. Furthermore, the developed sensor was able to discriminate the signal with and without the presence of pyocyanin directly in P. aeruginosa culture. This proposed technique demonstrates its potential application in monitoring the presence of P. aeruginosa infection in patients. Elsevier 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/87822/1/ABSTRACT.pdf Abdul Rashid, Jahwarhar Izuan and Kannan, Vayithiswary and Ahmad, Muhammad Hafiz and Mon, Aye Aye and Taufik, Safura and Miskon, Azizi and Ong, Keat Khim and Yusof, Nor Azah (2020) An electrochemical sensor based on gold nanoparticles-functionalized reduced graphene oxide screen printed electrode for the detection of pyocyanin biomarker in Pseudomonas aeruginosa infection. Materials Science & Engineering C-Materials for Biological Applications, 120. art. no. 111625. pp. 1-9. ISSN 0928-4931 https://www.sciencedirect.com/science/article/pii/S0928493120335438 10.1016/j.msec.2020.111625 |
| spellingShingle | Abdul Rashid, Jahwarhar Izuan Kannan, Vayithiswary Ahmad, Muhammad Hafiz Mon, Aye Aye Taufik, Safura Miskon, Azizi Ong, Keat Khim Yusof, Nor Azah An electrochemical sensor based on gold nanoparticles-functionalized reduced graphene oxide screen printed electrode for the detection of pyocyanin biomarker in Pseudomonas aeruginosa infection |
| title | An electrochemical sensor based on gold nanoparticles-functionalized reduced graphene oxide screen printed electrode for the detection of pyocyanin biomarker in Pseudomonas aeruginosa infection |
| title_full | An electrochemical sensor based on gold nanoparticles-functionalized reduced graphene oxide screen printed electrode for the detection of pyocyanin biomarker in Pseudomonas aeruginosa infection |
| title_fullStr | An electrochemical sensor based on gold nanoparticles-functionalized reduced graphene oxide screen printed electrode for the detection of pyocyanin biomarker in Pseudomonas aeruginosa infection |
| title_full_unstemmed | An electrochemical sensor based on gold nanoparticles-functionalized reduced graphene oxide screen printed electrode for the detection of pyocyanin biomarker in Pseudomonas aeruginosa infection |
| title_short | An electrochemical sensor based on gold nanoparticles-functionalized reduced graphene oxide screen printed electrode for the detection of pyocyanin biomarker in Pseudomonas aeruginosa infection |
| title_sort | electrochemical sensor based on gold nanoparticles-functionalized reduced graphene oxide screen printed electrode for the detection of pyocyanin biomarker in pseudomonas aeruginosa infection |
| url | http://psasir.upm.edu.my/id/eprint/87822/ http://psasir.upm.edu.my/id/eprint/87822/ http://psasir.upm.edu.my/id/eprint/87822/ http://psasir.upm.edu.my/id/eprint/87822/1/ABSTRACT.pdf |