Antioxidant and anti-obesity properties of local chilies varieties in Malaysia
Obesity is a serious health concern as it may initiate common chronic diseases. Chili pepper is an important spice that brings spiciness and commonly used in cuisines. However, the antioxidant and anti-obesity properties of chili varieties in Malaysia has not yet been fully investigated. Therefore,...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Springer Science
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/87709/ http://psasir.upm.edu.my/id/eprint/87709/1/ABSTRACT.pdf |
| _version_ | 1848860487108788224 |
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| author | Chan, Suk Huei Azlan, Azrina Ismail, Amin Shafie, Nurul Husna Sultana, Sharmin |
| author_facet | Chan, Suk Huei Azlan, Azrina Ismail, Amin Shafie, Nurul Husna Sultana, Sharmin |
| author_sort | Chan, Suk Huei |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Obesity is a serious health concern as it may initiate common chronic diseases. Chili pepper is an important spice that brings spiciness and commonly used in cuisines. However, the antioxidant and anti-obesity properties of chili varieties in Malaysia has not yet been fully investigated. Therefore, this study aimed to determine the antioxidant (content and activity) and anti-obesity properties of five different varieties of local chili peppers. The antioxidant activities of the extracts were determined through ferric-reducing antioxidant power (FRAP) and 2, 2'azinobis-(3-ethyl-benzothiazoline-6-sulphonic acid (ABTS) assays. Cell cytotoxicity of the selected chili extracts was determined in 3T3-L1 pre adipocytes using cell viability assay (MTT) assay. Whereas the ability to inhibit oil accumulation in fully differentiated 3T3-L1 adipocytes of the selected chili pepper extracts was assayed using Oil Red O staining. The results showed that Kulai 568 pulp extract had the highest level of total phenolic content (TPC) (47.88 ± 0.220 mg GAE/g), whereas Centil pulp extract had the highest level of total flavonoid content (TFC) (26.60 ± 0.52 mg QE/g). In term of antioxidant activities, Bara pulp extract had the highest value in FRAP (3.058 ± 0.002 mM Fe2+/mg extract) and ABTS (IC50 = 12.411 ± 0.025). High performance liquid chromatography (HPLC) analysis, Bara pulp extract has the highest level of capsaicin (72.271 ± 0.957 µg/ml). In terms of inhibition of oil accumulation Centil seed extract presented the best result (69.09-92.20%), while Bara pulp extract inhibited the most pancreatic lipase activity (IC50 = 4.84 ± 0.57 µg/ml). Thus, it is suggested that Centil seed and Bara pulp extracts can be a potent antioxidant and anti-obesity agents. |
| first_indexed | 2025-11-15T12:46:01Z |
| format | Article |
| id | upm-87709 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:46:01Z |
| publishDate | 2020 |
| publisher | Springer Science |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-877092022-07-06T02:56:23Z http://psasir.upm.edu.my/id/eprint/87709/ Antioxidant and anti-obesity properties of local chilies varieties in Malaysia Chan, Suk Huei Azlan, Azrina Ismail, Amin Shafie, Nurul Husna Sultana, Sharmin Obesity is a serious health concern as it may initiate common chronic diseases. Chili pepper is an important spice that brings spiciness and commonly used in cuisines. However, the antioxidant and anti-obesity properties of chili varieties in Malaysia has not yet been fully investigated. Therefore, this study aimed to determine the antioxidant (content and activity) and anti-obesity properties of five different varieties of local chili peppers. The antioxidant activities of the extracts were determined through ferric-reducing antioxidant power (FRAP) and 2, 2'azinobis-(3-ethyl-benzothiazoline-6-sulphonic acid (ABTS) assays. Cell cytotoxicity of the selected chili extracts was determined in 3T3-L1 pre adipocytes using cell viability assay (MTT) assay. Whereas the ability to inhibit oil accumulation in fully differentiated 3T3-L1 adipocytes of the selected chili pepper extracts was assayed using Oil Red O staining. The results showed that Kulai 568 pulp extract had the highest level of total phenolic content (TPC) (47.88 ± 0.220 mg GAE/g), whereas Centil pulp extract had the highest level of total flavonoid content (TFC) (26.60 ± 0.52 mg QE/g). In term of antioxidant activities, Bara pulp extract had the highest value in FRAP (3.058 ± 0.002 mM Fe2+/mg extract) and ABTS (IC50 = 12.411 ± 0.025). High performance liquid chromatography (HPLC) analysis, Bara pulp extract has the highest level of capsaicin (72.271 ± 0.957 µg/ml). In terms of inhibition of oil accumulation Centil seed extract presented the best result (69.09-92.20%), while Bara pulp extract inhibited the most pancreatic lipase activity (IC50 = 4.84 ± 0.57 µg/ml). Thus, it is suggested that Centil seed and Bara pulp extracts can be a potent antioxidant and anti-obesity agents. Springer Science 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/87709/1/ABSTRACT.pdf Chan, Suk Huei and Azlan, Azrina and Ismail, Amin and Shafie, Nurul Husna and Sultana, Sharmin (2020) Antioxidant and anti-obesity properties of local chilies varieties in Malaysia. Journal of Food Science and Technology, 57 (10). 3677 - 3687. ISSN 0022-1155; ESSN: 0975-8402 https://link.springer.com/article/10.1007/s13197-020-04400-x 10.1007/s13197-020-04400-x |
| spellingShingle | Chan, Suk Huei Azlan, Azrina Ismail, Amin Shafie, Nurul Husna Sultana, Sharmin Antioxidant and anti-obesity properties of local chilies varieties in Malaysia |
| title | Antioxidant and anti-obesity properties of local chilies varieties in Malaysia |
| title_full | Antioxidant and anti-obesity properties of local chilies varieties in Malaysia |
| title_fullStr | Antioxidant and anti-obesity properties of local chilies varieties in Malaysia |
| title_full_unstemmed | Antioxidant and anti-obesity properties of local chilies varieties in Malaysia |
| title_short | Antioxidant and anti-obesity properties of local chilies varieties in Malaysia |
| title_sort | antioxidant and anti-obesity properties of local chilies varieties in malaysia |
| url | http://psasir.upm.edu.my/id/eprint/87709/ http://psasir.upm.edu.my/id/eprint/87709/ http://psasir.upm.edu.my/id/eprint/87709/ http://psasir.upm.edu.my/id/eprint/87709/1/ABSTRACT.pdf |