Segregation analysis for bacterial leaf blight disease resistance genes in rice ‘MR219’ using SSR marker
Bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae is one of the major hindrances in rice (Oryza sativa L.) production across the world including Malaysia. Therefore, the development of disease-resistant varieties remains a very economical and effective method of controlling BLB in...
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| Format: | Article |
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Instituto de Investigaciones Agropecuarias
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/87547/ |
| _version_ | 1848860456347762688 |
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| author | Mohd Zuki, Zakiah Yusop, Mohd Rafii Ramli, Asfaliza Yusuff, Oladosu Latif, Mohammad Abdul Sijam, Kamaruzaman Ismail, Mohd Razi Mohd Sarif, Hamidah |
| author_facet | Mohd Zuki, Zakiah Yusop, Mohd Rafii Ramli, Asfaliza Yusuff, Oladosu Latif, Mohammad Abdul Sijam, Kamaruzaman Ismail, Mohd Razi Mohd Sarif, Hamidah |
| author_sort | Mohd Zuki, Zakiah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae is one of the major hindrances in rice (Oryza sativa L.) production across the world including Malaysia. Therefore, the development of disease-resistant varieties remains a very economical and effective method of controlling BLB in rice. Based on this background, this study was conducted to analyze segregation pattern of simple-sequence repeat (SSR) markers associated with BLB resistance genes in an F2 bulk population derived from a resistant variety (IRBB60) and a susceptible variety (MR219). Out of 129 simple sequence repeat (SSR) markers screened, 18 distinct polymorphism markers including R-gene based markers were used to screen 345 F2 progenies for resistance to BLB. Among the polymorphic markers, the chi-square analyses showed that 15 SSR markers (i.e. RM13 (xa5), RM21 (Xa21), RM122 (xa5), RM153 (xa5), RM164 (Xa13), RM206 (Xa10), RM5509, RM20B, RM25, RM163, RM169, RM218, RM267, RM276, and RM334) had a segregation ratio of 1:2:1 for a single gene model (df = 2.0, p ≤ 0.05). For phenotypic ratio, the F2 population segregated in ratio 3:1 (R:S) for resistant and susceptible plants, respectively. This indicated that resistance to BLB caused by pathotype X. oryzae pv. oryzae (Xoo) in the ‘MR219’ × ‘IRBB60’ F2 population is controlled by single dominant genes. The result presented in this study will help breeders to further breeding research in rice by enabling selection based on the genotype rather than on the phenotype. Similarly, the markers reported in this study will serve as a valuable tool for marker-aided selection for BLB resistance gene. |
| first_indexed | 2025-11-15T12:45:31Z |
| format | Article |
| id | upm-87547 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T12:45:31Z |
| publishDate | 2020 |
| publisher | Instituto de Investigaciones Agropecuarias |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-875472022-11-23T03:15:06Z http://psasir.upm.edu.my/id/eprint/87547/ Segregation analysis for bacterial leaf blight disease resistance genes in rice ‘MR219’ using SSR marker Mohd Zuki, Zakiah Yusop, Mohd Rafii Ramli, Asfaliza Yusuff, Oladosu Latif, Mohammad Abdul Sijam, Kamaruzaman Ismail, Mohd Razi Mohd Sarif, Hamidah Bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae is one of the major hindrances in rice (Oryza sativa L.) production across the world including Malaysia. Therefore, the development of disease-resistant varieties remains a very economical and effective method of controlling BLB in rice. Based on this background, this study was conducted to analyze segregation pattern of simple-sequence repeat (SSR) markers associated with BLB resistance genes in an F2 bulk population derived from a resistant variety (IRBB60) and a susceptible variety (MR219). Out of 129 simple sequence repeat (SSR) markers screened, 18 distinct polymorphism markers including R-gene based markers were used to screen 345 F2 progenies for resistance to BLB. Among the polymorphic markers, the chi-square analyses showed that 15 SSR markers (i.e. RM13 (xa5), RM21 (Xa21), RM122 (xa5), RM153 (xa5), RM164 (Xa13), RM206 (Xa10), RM5509, RM20B, RM25, RM163, RM169, RM218, RM267, RM276, and RM334) had a segregation ratio of 1:2:1 for a single gene model (df = 2.0, p ≤ 0.05). For phenotypic ratio, the F2 population segregated in ratio 3:1 (R:S) for resistant and susceptible plants, respectively. This indicated that resistance to BLB caused by pathotype X. oryzae pv. oryzae (Xoo) in the ‘MR219’ × ‘IRBB60’ F2 population is controlled by single dominant genes. The result presented in this study will help breeders to further breeding research in rice by enabling selection based on the genotype rather than on the phenotype. Similarly, the markers reported in this study will serve as a valuable tool for marker-aided selection for BLB resistance gene. Instituto de Investigaciones Agropecuarias 2020-06 Article PeerReviewed Mohd Zuki, Zakiah and Yusop, Mohd Rafii and Ramli, Asfaliza and Yusuff, Oladosu and Latif, Mohammad Abdul and Sijam, Kamaruzaman and Ismail, Mohd Razi and Mohd Sarif, Hamidah (2020) Segregation analysis for bacterial leaf blight disease resistance genes in rice ‘MR219’ using SSR marker. Chilean Journal of Agricultural Research, 80 (2). 227 - 233. ISSN 0718-5839 https://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392020000200227 10.4067/S0718-58392020000200227 |
| spellingShingle | Mohd Zuki, Zakiah Yusop, Mohd Rafii Ramli, Asfaliza Yusuff, Oladosu Latif, Mohammad Abdul Sijam, Kamaruzaman Ismail, Mohd Razi Mohd Sarif, Hamidah Segregation analysis for bacterial leaf blight disease resistance genes in rice ‘MR219’ using SSR marker |
| title | Segregation analysis for bacterial leaf blight disease resistance genes in rice ‘MR219’ using SSR marker |
| title_full | Segregation analysis for bacterial leaf blight disease resistance genes in rice ‘MR219’ using SSR marker |
| title_fullStr | Segregation analysis for bacterial leaf blight disease resistance genes in rice ‘MR219’ using SSR marker |
| title_full_unstemmed | Segregation analysis for bacterial leaf blight disease resistance genes in rice ‘MR219’ using SSR marker |
| title_short | Segregation analysis for bacterial leaf blight disease resistance genes in rice ‘MR219’ using SSR marker |
| title_sort | segregation analysis for bacterial leaf blight disease resistance genes in rice ‘mr219’ using ssr marker |
| url | http://psasir.upm.edu.my/id/eprint/87547/ http://psasir.upm.edu.my/id/eprint/87547/ http://psasir.upm.edu.my/id/eprint/87547/ |