Scoring system for lesions induced by different strains of infectious bursal disease virus in chicken
Newcastle disease virus strains are velogenic, mesogenic, and lentogenic. This study aims to design a scoring system for lesions induced by different strains of Newcastle disease virus in chicken. Three experiments were conducted. In experiments 1 and 2, chickens were divided into infected and contr...
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| Format: | Article |
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Ansi net
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/87532/ |
| _version_ | 1848860452566597632 |
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| author | Hussein, Elawad A. Bejo, Mohd Hair Omar, Abdul Rahman Liew, Pit Sze Saeed, Mohammed Ibrahim Arshad, Siti Suri Awad, Elmutaz Atta Ideris, Aini |
| author_facet | Hussein, Elawad A. Bejo, Mohd Hair Omar, Abdul Rahman Liew, Pit Sze Saeed, Mohammed Ibrahim Arshad, Siti Suri Awad, Elmutaz Atta Ideris, Aini |
| author_sort | Hussein, Elawad A. |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Newcastle disease virus strains are velogenic, mesogenic, and lentogenic. This study aims to design a scoring system for lesions induced by different strains of Newcastle disease virus in chicken. Three experiments were conducted. In experiments 1 and 2, chickens were divided into infected and control groups. Infected groups of experiments 1 and 2 consisted of 6 and 24 specific pathogen-free (SPF) chickens, respectively. Control groups in experiments 1 and 2 consisted of 6 and 15 SPF chickens, respectively. In infected groups, infection was induced by intranasal administration of 105 50% EID50/0.1 mL of velogenic Newcastle disease virus strain (vNDV). Infected chickens in experiment 1 were euthanised by cervical dislocation on days 3, 6, and 7 postinoculation (pi). Infected chickens in experiment 2 were euthanised at hours (hrs) 2, 4, 6, 12 and days 1, 2, 4, and 6 pi. Chickens of the control group in experiment 1 were euthanised on days 3 and 7 pi, whereas control group chickens in experiment 2 were euthanised on days 0, 1, 2, 4, and 6 pi. Then in experiment 3, 15 SPF chickens were divided into three groups; in the first group, 5 SPF chickens were infected with vNDV, in the second group, 5 SPF chickens were infected Background and Objective: There are 3 pathogenic strains of infectious bursal disease virus (IBDV), which are classical, variant and very virulent strains. The objective of this study was to design a scoring system for lesions induced by different strains of infectious bursal disease virus in chicken. Materials and Methods: Three experiments were conducted. In experiments 1 and 2, chickens were divided into infected and control groups. Infected groups of experiments 1 and 2 consisted of 15 and 24 specific pathogen-free (SPF) chickens, respectively. Infection was induced by oral administration of 107.5 50% EID50/0.1 mL of very virulent IBDV (vvIBDV). Infected chickens in experiment 1 were euthanised by cervical dislocation on days 1, 2, 3, 4 and 5 post-inoculation (pi). Infected chickens in experiment 2 were euthanised at hours (h) 2, 4, 6, 12 and days 1, 2, 4 and 6 pi. Control groups in experiments 1 and 2 consisted of 6 and 15 SPF chickens, respectively. Chickens of the control group in experiment 1 were euthanised on days 1 and 5 pi, whereas control group chickens in experiment 2 were euthanised on days 0, 1, 2, 4 and 6 pi. Then, 20 SPF chickens, in experiment 3, were divided into three groups; in the first group, 10 SPF chickens were infected with vvIBDV, in the second group, 5 SPF chickens were infected with classical IBDV (caIBDV) (103.0 EID50/0.1 mL) and the third group of chicken was kept as a control group without infection. Five chickens from first group, five chickens of second group and five chicken of the third group were euthanised on day 4 pi. Another 5 chickens from first group were euthanized on day 7 pi. In all previous experiments, tissues of bursa of Fabricius, caecal tonsil, liver, kidney, spleen, junction of proventriculus and gizzard, intestine, muscle and thymus were collected, fixed in 10% buffered formalin, embedded in paraffin and sectioned. HS staining was applied. Results: When the strain of the virus was vvIBDV, the scoring was always greater in comparison with the strain caIBDV. The lesions induced in the bursa of Fabricius by vvIBDV strain, when the infection was chronic, were determined. Conclusion: A scoring system for the lesions induced by different strains of IBDV in 9 different tissues was expected to be beneficial in the field of histopathology. |
| first_indexed | 2025-11-15T12:45:28Z |
| format | Article |
| id | upm-87532 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T12:45:28Z |
| publishDate | 2020 |
| publisher | Ansi net |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-875322022-11-23T02:28:37Z http://psasir.upm.edu.my/id/eprint/87532/ Scoring system for lesions induced by different strains of infectious bursal disease virus in chicken Hussein, Elawad A. Bejo, Mohd Hair Omar, Abdul Rahman Liew, Pit Sze Saeed, Mohammed Ibrahim Arshad, Siti Suri Awad, Elmutaz Atta Ideris, Aini Newcastle disease virus strains are velogenic, mesogenic, and lentogenic. This study aims to design a scoring system for lesions induced by different strains of Newcastle disease virus in chicken. Three experiments were conducted. In experiments 1 and 2, chickens were divided into infected and control groups. Infected groups of experiments 1 and 2 consisted of 6 and 24 specific pathogen-free (SPF) chickens, respectively. Control groups in experiments 1 and 2 consisted of 6 and 15 SPF chickens, respectively. In infected groups, infection was induced by intranasal administration of 105 50% EID50/0.1 mL of velogenic Newcastle disease virus strain (vNDV). Infected chickens in experiment 1 were euthanised by cervical dislocation on days 3, 6, and 7 postinoculation (pi). Infected chickens in experiment 2 were euthanised at hours (hrs) 2, 4, 6, 12 and days 1, 2, 4, and 6 pi. Chickens of the control group in experiment 1 were euthanised on days 3 and 7 pi, whereas control group chickens in experiment 2 were euthanised on days 0, 1, 2, 4, and 6 pi. Then in experiment 3, 15 SPF chickens were divided into three groups; in the first group, 5 SPF chickens were infected with vNDV, in the second group, 5 SPF chickens were infected Background and Objective: There are 3 pathogenic strains of infectious bursal disease virus (IBDV), which are classical, variant and very virulent strains. The objective of this study was to design a scoring system for lesions induced by different strains of infectious bursal disease virus in chicken. Materials and Methods: Three experiments were conducted. In experiments 1 and 2, chickens were divided into infected and control groups. Infected groups of experiments 1 and 2 consisted of 15 and 24 specific pathogen-free (SPF) chickens, respectively. Infection was induced by oral administration of 107.5 50% EID50/0.1 mL of very virulent IBDV (vvIBDV). Infected chickens in experiment 1 were euthanised by cervical dislocation on days 1, 2, 3, 4 and 5 post-inoculation (pi). Infected chickens in experiment 2 were euthanised at hours (h) 2, 4, 6, 12 and days 1, 2, 4 and 6 pi. Control groups in experiments 1 and 2 consisted of 6 and 15 SPF chickens, respectively. Chickens of the control group in experiment 1 were euthanised on days 1 and 5 pi, whereas control group chickens in experiment 2 were euthanised on days 0, 1, 2, 4 and 6 pi. Then, 20 SPF chickens, in experiment 3, were divided into three groups; in the first group, 10 SPF chickens were infected with vvIBDV, in the second group, 5 SPF chickens were infected with classical IBDV (caIBDV) (103.0 EID50/0.1 mL) and the third group of chicken was kept as a control group without infection. Five chickens from first group, five chickens of second group and five chicken of the third group were euthanised on day 4 pi. Another 5 chickens from first group were euthanized on day 7 pi. In all previous experiments, tissues of bursa of Fabricius, caecal tonsil, liver, kidney, spleen, junction of proventriculus and gizzard, intestine, muscle and thymus were collected, fixed in 10% buffered formalin, embedded in paraffin and sectioned. HS staining was applied. Results: When the strain of the virus was vvIBDV, the scoring was always greater in comparison with the strain caIBDV. The lesions induced in the bursa of Fabricius by vvIBDV strain, when the infection was chronic, were determined. Conclusion: A scoring system for the lesions induced by different strains of IBDV in 9 different tissues was expected to be beneficial in the field of histopathology. Ansi net 2020 Article PeerReviewed Hussein, Elawad A. and Bejo, Mohd Hair and Omar, Abdul Rahman and Liew, Pit Sze and Saeed, Mohammed Ibrahim and Arshad, Siti Suri and Awad, Elmutaz Atta and Ideris, Aini (2020) Scoring system for lesions induced by different strains of infectious bursal disease virus in chicken. International Journal of Poultry Science, 19. 153 - 160. ISSN 1682-8356 https://scialert.net/fulltext/?doi=ijps.2020.153.160 10.3923/ijps.2020.153.160 |
| spellingShingle | Hussein, Elawad A. Bejo, Mohd Hair Omar, Abdul Rahman Liew, Pit Sze Saeed, Mohammed Ibrahim Arshad, Siti Suri Awad, Elmutaz Atta Ideris, Aini Scoring system for lesions induced by different strains of infectious bursal disease virus in chicken |
| title | Scoring system for lesions induced by different strains of infectious bursal disease virus in chicken |
| title_full | Scoring system for lesions induced by different strains of infectious bursal disease virus in chicken |
| title_fullStr | Scoring system for lesions induced by different strains of infectious bursal disease virus in chicken |
| title_full_unstemmed | Scoring system for lesions induced by different strains of infectious bursal disease virus in chicken |
| title_short | Scoring system for lesions induced by different strains of infectious bursal disease virus in chicken |
| title_sort | scoring system for lesions induced by different strains of infectious bursal disease virus in chicken |
| url | http://psasir.upm.edu.my/id/eprint/87532/ http://psasir.upm.edu.my/id/eprint/87532/ http://psasir.upm.edu.my/id/eprint/87532/ |