Response surface methodology optimisation and kinetics of diesel degradation by a cold-adapted antarctic bacterium, Arthrobacter sp. Strain AQ5-05
Petroleum hydrocarbons, notably diesel oil, are the main energy source for running amenities in the Antarctic region and are the major cause of pollution in this area. Diesel oil spills are one of the major challenges facing management of the Antarctic environment. Bioremediation using bacteria can...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
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MDPI
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/87467/ |
| _version_ | 1848860444165406720 |
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| author | Abdulrasheed, Mansur Zulkharnain, Azham Zakaria, Nur Nadhirah Ahmad Roslee, Ahmad Fareez Abdul Khalil, Khalilah Napis, Suhaimi Convey, Peter Gomez-Fuentes, Claudio Ahmad, Siti Aqlima |
| author_facet | Abdulrasheed, Mansur Zulkharnain, Azham Zakaria, Nur Nadhirah Ahmad Roslee, Ahmad Fareez Abdul Khalil, Khalilah Napis, Suhaimi Convey, Peter Gomez-Fuentes, Claudio Ahmad, Siti Aqlima |
| author_sort | Abdulrasheed, Mansur |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Petroleum hydrocarbons, notably diesel oil, are the main energy source for running amenities in the Antarctic region and are the major cause of pollution in this area. Diesel oil spills are one of the major challenges facing management of the Antarctic environment. Bioremediation using bacteria can be an effective and eco-friendly approach for their remediation. However, since the introduction of non-native organisms, including microorganisms, into the Antarctic or between the distinct biogeographical regions within the continent is not permitted under the Antarctic Treaty, it is crucial to discover native oil-degrading, psychrotolerant microorganisms that can be used in diesel bioremediation. The primary aim of the current study is to optimize the conditions for growth and diesel degradation activity of an Antarctic local bacterium, Arthrobacter sp. strain AQ5-05, using the Plackett-Burman approach and response surface method (RSM) via a central composite design (CCD) approach. Based on this approach, temperature, pH, and salinity were calculated to be optimum at 16.30 °C, pH 7.67 and 1.12% (w/v), respectively. A second order polynomial regression model very accurately represented the experimental figures’ interpretation. These optimized environmental conditions increased diesel degradation from 34.5% (at 10 °C, pH 7.00 and 1.00% (w/v) salinity) to 56.4%. Further investigation of the kinetics of diesel reduction by strain AQ5-05 revealed that the Teissier model had the lowest RMSE and AICC values. The calculated values for the Teissier constants of maximal growth rate, half-saturation rate constant for the maximal growth, and half inhibition constants (μmax, Ks, and Ki), were 0.999 h−1, 1.971% (v/v) and 1.764% (v/v), respectively. The data obtained therefore confirmed the potential application of this cold-tolerant strain in the bioremediation of diesel-contaminated Antarctic soils at low temperature. |
| first_indexed | 2025-11-15T12:45:20Z |
| format | Article |
| id | upm-87467 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T12:45:20Z |
| publishDate | 2020 |
| publisher | MDPI |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-874672023-05-30T02:35:48Z http://psasir.upm.edu.my/id/eprint/87467/ Response surface methodology optimisation and kinetics of diesel degradation by a cold-adapted antarctic bacterium, Arthrobacter sp. Strain AQ5-05 Abdulrasheed, Mansur Zulkharnain, Azham Zakaria, Nur Nadhirah Ahmad Roslee, Ahmad Fareez Abdul Khalil, Khalilah Napis, Suhaimi Convey, Peter Gomez-Fuentes, Claudio Ahmad, Siti Aqlima Petroleum hydrocarbons, notably diesel oil, are the main energy source for running amenities in the Antarctic region and are the major cause of pollution in this area. Diesel oil spills are one of the major challenges facing management of the Antarctic environment. Bioremediation using bacteria can be an effective and eco-friendly approach for their remediation. However, since the introduction of non-native organisms, including microorganisms, into the Antarctic or between the distinct biogeographical regions within the continent is not permitted under the Antarctic Treaty, it is crucial to discover native oil-degrading, psychrotolerant microorganisms that can be used in diesel bioremediation. The primary aim of the current study is to optimize the conditions for growth and diesel degradation activity of an Antarctic local bacterium, Arthrobacter sp. strain AQ5-05, using the Plackett-Burman approach and response surface method (RSM) via a central composite design (CCD) approach. Based on this approach, temperature, pH, and salinity were calculated to be optimum at 16.30 °C, pH 7.67 and 1.12% (w/v), respectively. A second order polynomial regression model very accurately represented the experimental figures’ interpretation. These optimized environmental conditions increased diesel degradation from 34.5% (at 10 °C, pH 7.00 and 1.00% (w/v) salinity) to 56.4%. Further investigation of the kinetics of diesel reduction by strain AQ5-05 revealed that the Teissier model had the lowest RMSE and AICC values. The calculated values for the Teissier constants of maximal growth rate, half-saturation rate constant for the maximal growth, and half inhibition constants (μmax, Ks, and Ki), were 0.999 h−1, 1.971% (v/v) and 1.764% (v/v), respectively. The data obtained therefore confirmed the potential application of this cold-tolerant strain in the bioremediation of diesel-contaminated Antarctic soils at low temperature. MDPI 2020-08 Article PeerReviewed Abdulrasheed, Mansur and Zulkharnain, Azham and Zakaria, Nur Nadhirah and Ahmad Roslee, Ahmad Fareez and Abdul Khalil, Khalilah and Napis, Suhaimi and Convey, Peter and Gomez-Fuentes, Claudio and Ahmad, Siti Aqlima (2020) Response surface methodology optimisation and kinetics of diesel degradation by a cold-adapted antarctic bacterium, Arthrobacter sp. Strain AQ5-05. Sustainability, 12 (17). pp. 1-14. ISSN 2071-1050 https://www.mdpi.com/2071-1050/12/17/6966 10.3390/su12176966 |
| spellingShingle | Abdulrasheed, Mansur Zulkharnain, Azham Zakaria, Nur Nadhirah Ahmad Roslee, Ahmad Fareez Abdul Khalil, Khalilah Napis, Suhaimi Convey, Peter Gomez-Fuentes, Claudio Ahmad, Siti Aqlima Response surface methodology optimisation and kinetics of diesel degradation by a cold-adapted antarctic bacterium, Arthrobacter sp. Strain AQ5-05 |
| title | Response surface methodology optimisation and kinetics of diesel degradation by a cold-adapted antarctic bacterium, Arthrobacter sp. Strain AQ5-05 |
| title_full | Response surface methodology optimisation and kinetics of diesel degradation by a cold-adapted antarctic bacterium, Arthrobacter sp. Strain AQ5-05 |
| title_fullStr | Response surface methodology optimisation and kinetics of diesel degradation by a cold-adapted antarctic bacterium, Arthrobacter sp. Strain AQ5-05 |
| title_full_unstemmed | Response surface methodology optimisation and kinetics of diesel degradation by a cold-adapted antarctic bacterium, Arthrobacter sp. Strain AQ5-05 |
| title_short | Response surface methodology optimisation and kinetics of diesel degradation by a cold-adapted antarctic bacterium, Arthrobacter sp. Strain AQ5-05 |
| title_sort | response surface methodology optimisation and kinetics of diesel degradation by a cold-adapted antarctic bacterium, arthrobacter sp. strain aq5-05 |
| url | http://psasir.upm.edu.my/id/eprint/87467/ http://psasir.upm.edu.my/id/eprint/87467/ http://psasir.upm.edu.my/id/eprint/87467/ |