Response surface methodology for the optimization of Keratinase production in culture medium containing feathers by Bacillus sp. UPM-AAG1
Keratinase is a type of proteolytic enzyme with broad application in industry. The main objective of this work is the optimization of keratinase production from Bacillus sp. strain UPM-AAG1 using Plackett-Burman (PB) and central composite design (CCD) for parameters, such as pH, temperature, feather...
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| Format: | Article |
| Language: | English |
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MDPI
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/87466/ http://psasir.upm.edu.my/id/eprint/87466/1/87466.pdf |
| _version_ | 1848860443873902592 |
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| author | Abdul Gafar, Aa’ishah Khayat, Mohd Ezuan Ahmad, Siti Aqlima Yasid, Nur Adeela |
| author_facet | Abdul Gafar, Aa’ishah Khayat, Mohd Ezuan Ahmad, Siti Aqlima Yasid, Nur Adeela |
| author_sort | Abdul Gafar, Aa’ishah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Keratinase is a type of proteolytic enzyme with broad application in industry. The main objective of this work is the optimization of keratinase production from Bacillus sp. strain UPM-AAG1 using Plackett-Burman (PB) and central composite design (CCD) for parameters, such as pH, temperature, feather concentration, and inoculum size. The optimum points for temperature, pH, and inoculum and feather concentrations were 31.66 °C, 6.87, 5.01 (w/v), and 4.53 (w/v), respectively, with an optimum keratinase activity of 60.55 U/mL. The keratinase activity was further numerically optimized for commercial application. The best numerical solution recommended a pH of 5.84, temperature of 25 °C, inoculums’ size of 5.0 (v/v), feather concentration of 4.97 (w/v). Optimization resulted an activity of 56.218 U/mL with the desirability value of 0.968. Amino acid analysis profile revealed the presence of essential and non-essential amino acids. These properties make Bacillus sp. UPM-AAG1 a potential bacterium to be used locally for the production of keratinase from feather waste. |
| first_indexed | 2025-11-15T12:45:19Z |
| format | Article |
| id | upm-87466 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:45:19Z |
| publishDate | 2020 |
| publisher | MDPI |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-874662025-02-10T02:29:15Z http://psasir.upm.edu.my/id/eprint/87466/ Response surface methodology for the optimization of Keratinase production in culture medium containing feathers by Bacillus sp. UPM-AAG1 Abdul Gafar, Aa’ishah Khayat, Mohd Ezuan Ahmad, Siti Aqlima Yasid, Nur Adeela Keratinase is a type of proteolytic enzyme with broad application in industry. The main objective of this work is the optimization of keratinase production from Bacillus sp. strain UPM-AAG1 using Plackett-Burman (PB) and central composite design (CCD) for parameters, such as pH, temperature, feather concentration, and inoculum size. The optimum points for temperature, pH, and inoculum and feather concentrations were 31.66 °C, 6.87, 5.01 (w/v), and 4.53 (w/v), respectively, with an optimum keratinase activity of 60.55 U/mL. The keratinase activity was further numerically optimized for commercial application. The best numerical solution recommended a pH of 5.84, temperature of 25 °C, inoculums’ size of 5.0 (v/v), feather concentration of 4.97 (w/v). Optimization resulted an activity of 56.218 U/mL with the desirability value of 0.968. Amino acid analysis profile revealed the presence of essential and non-essential amino acids. These properties make Bacillus sp. UPM-AAG1 a potential bacterium to be used locally for the production of keratinase from feather waste. MDPI 2020-07-29 Article PeerReviewed text en cc_by_4 http://psasir.upm.edu.my/id/eprint/87466/1/87466.pdf Abdul Gafar, Aa’ishah and Khayat, Mohd Ezuan and Ahmad, Siti Aqlima and Yasid, Nur Adeela (2020) Response surface methodology for the optimization of Keratinase production in culture medium containing feathers by Bacillus sp. UPM-AAG1. Catalysts, 10 (8). art. no. 848. pp. 1-18. ISSN 2073-4344; eISSN: 2073-4344 https://www.mdpi.com/2073-4344/10/8/848 10.3390/catal10080848 |
| spellingShingle | Abdul Gafar, Aa’ishah Khayat, Mohd Ezuan Ahmad, Siti Aqlima Yasid, Nur Adeela Response surface methodology for the optimization of Keratinase production in culture medium containing feathers by Bacillus sp. UPM-AAG1 |
| title | Response surface methodology for the optimization of Keratinase production in culture medium containing feathers by Bacillus sp. UPM-AAG1 |
| title_full | Response surface methodology for the optimization of Keratinase production in culture medium containing feathers by Bacillus sp. UPM-AAG1 |
| title_fullStr | Response surface methodology for the optimization of Keratinase production in culture medium containing feathers by Bacillus sp. UPM-AAG1 |
| title_full_unstemmed | Response surface methodology for the optimization of Keratinase production in culture medium containing feathers by Bacillus sp. UPM-AAG1 |
| title_short | Response surface methodology for the optimization of Keratinase production in culture medium containing feathers by Bacillus sp. UPM-AAG1 |
| title_sort | response surface methodology for the optimization of keratinase production in culture medium containing feathers by bacillus sp. upm-aag1 |
| url | http://psasir.upm.edu.my/id/eprint/87466/ http://psasir.upm.edu.my/id/eprint/87466/ http://psasir.upm.edu.my/id/eprint/87466/ http://psasir.upm.edu.my/id/eprint/87466/1/87466.pdf |