Protective effects of stem cells from human exfoliated deciduous teeth derived conditioned medium on osteoarthritic chondrocytes
Treatment of osteoarthritis (OA) is still a major clinical challenge due to the limited inherent healing capacity of cartilage. Recent studies utilising stem cells suggest that the therapeutic benefits of these cells are mediated through the paracrine mechanism of bioactive molecules. The present st...
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| Format: | Article |
| Language: | English |
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PLOS ONE
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/87344/ http://psasir.upm.edu.my/id/eprint/87344/1/Protective%20effects%20of%20stem%20cells%20from%20human%20exfoliated.pdf |
| _version_ | 1848860421596905472 |
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| author | Muhammad, Suleiman Alhaji Nordin, Norshariza Hussin, Paisal Mehat, Muhammad Zulfadli Abu Kasim, Noor Hayaty Fakurazi, Sharida |
| author_facet | Muhammad, Suleiman Alhaji Nordin, Norshariza Hussin, Paisal Mehat, Muhammad Zulfadli Abu Kasim, Noor Hayaty Fakurazi, Sharida |
| author_sort | Muhammad, Suleiman Alhaji |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Treatment of osteoarthritis (OA) is still a major clinical challenge due to the limited inherent healing capacity of cartilage. Recent studies utilising stem cells suggest that the therapeutic benefits of these cells are mediated through the paracrine mechanism of bioactive molecules. The present study evaluates the regenerative effect of stem cells from human exfoliated deciduous teeth (SHED) conditioned medium (CM) on OA chondrocytes. The CM was collected after the SHED were cultured in serum-free medium (SFM) for 48 or 72 h and the cells were characterised by the expression of MSC and pluripotency markers. Chondrocytes were stimulated with interleukin-1β and treated with the CM. Subsequently, the expression of aggrecan, collagen type 2 (COL 2), matrix metalloproteinase-13 (MMP-13), nuclear factor-kB (NF-kB) and the level of inflammatory and anti-inflammatory markers were evaluated. SHED expressed mesenchymal stromal cell surface proteins but were negative for haematopoietic markers. SHED also showed protein expression of NANOG, OCT4 and SOX2 with differential subcellular localisation. Treatment of OA chondrocytes with CM enhanced anti-inflammation compared to control cells treated with SFM. Furthermore, the expression of MMP-13 and NF-kB was significantly downregulated in stimulated chondrocytes incubated in CM. The study also revealed that CM increased the expression of aggrecan and COL 2 in OA chondrocytes compared to SFM control. Both CM regenerate extracellular matrix proteins and mitigate increased MMP-13 expression through inhibition of NF-kB in OA chondrocytes due to the presence of bioactive molecules. The study underscores the potential of CM for OA treatment. |
| first_indexed | 2025-11-15T12:44:58Z |
| format | Article |
| id | upm-87344 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:44:58Z |
| publishDate | 2020 |
| publisher | PLOS ONE |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-873442022-11-07T09:06:53Z http://psasir.upm.edu.my/id/eprint/87344/ Protective effects of stem cells from human exfoliated deciduous teeth derived conditioned medium on osteoarthritic chondrocytes Muhammad, Suleiman Alhaji Nordin, Norshariza Hussin, Paisal Mehat, Muhammad Zulfadli Abu Kasim, Noor Hayaty Fakurazi, Sharida Treatment of osteoarthritis (OA) is still a major clinical challenge due to the limited inherent healing capacity of cartilage. Recent studies utilising stem cells suggest that the therapeutic benefits of these cells are mediated through the paracrine mechanism of bioactive molecules. The present study evaluates the regenerative effect of stem cells from human exfoliated deciduous teeth (SHED) conditioned medium (CM) on OA chondrocytes. The CM was collected after the SHED were cultured in serum-free medium (SFM) for 48 or 72 h and the cells were characterised by the expression of MSC and pluripotency markers. Chondrocytes were stimulated with interleukin-1β and treated with the CM. Subsequently, the expression of aggrecan, collagen type 2 (COL 2), matrix metalloproteinase-13 (MMP-13), nuclear factor-kB (NF-kB) and the level of inflammatory and anti-inflammatory markers were evaluated. SHED expressed mesenchymal stromal cell surface proteins but were negative for haematopoietic markers. SHED also showed protein expression of NANOG, OCT4 and SOX2 with differential subcellular localisation. Treatment of OA chondrocytes with CM enhanced anti-inflammation compared to control cells treated with SFM. Furthermore, the expression of MMP-13 and NF-kB was significantly downregulated in stimulated chondrocytes incubated in CM. The study also revealed that CM increased the expression of aggrecan and COL 2 in OA chondrocytes compared to SFM control. Both CM regenerate extracellular matrix proteins and mitigate increased MMP-13 expression through inhibition of NF-kB in OA chondrocytes due to the presence of bioactive molecules. The study underscores the potential of CM for OA treatment. PLOS ONE 2020-09-04 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/87344/1/Protective%20effects%20of%20stem%20cells%20from%20human%20exfoliated.pdf Muhammad, Suleiman Alhaji and Nordin, Norshariza and Hussin, Paisal and Mehat, Muhammad Zulfadli and Abu Kasim, Noor Hayaty and Fakurazi, Sharida (2020) Protective effects of stem cells from human exfoliated deciduous teeth derived conditioned medium on osteoarthritic chondrocytes. PLoS One, 15 (9). 238449 - 238459. ISSN 1932-6203 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0238449 10.1371/journal.pone.0238449 |
| spellingShingle | Muhammad, Suleiman Alhaji Nordin, Norshariza Hussin, Paisal Mehat, Muhammad Zulfadli Abu Kasim, Noor Hayaty Fakurazi, Sharida Protective effects of stem cells from human exfoliated deciduous teeth derived conditioned medium on osteoarthritic chondrocytes |
| title | Protective effects of stem cells from human exfoliated deciduous teeth derived conditioned medium on osteoarthritic chondrocytes |
| title_full | Protective effects of stem cells from human exfoliated deciduous teeth derived conditioned medium on osteoarthritic chondrocytes |
| title_fullStr | Protective effects of stem cells from human exfoliated deciduous teeth derived conditioned medium on osteoarthritic chondrocytes |
| title_full_unstemmed | Protective effects of stem cells from human exfoliated deciduous teeth derived conditioned medium on osteoarthritic chondrocytes |
| title_short | Protective effects of stem cells from human exfoliated deciduous teeth derived conditioned medium on osteoarthritic chondrocytes |
| title_sort | protective effects of stem cells from human exfoliated deciduous teeth derived conditioned medium on osteoarthritic chondrocytes |
| url | http://psasir.upm.edu.my/id/eprint/87344/ http://psasir.upm.edu.my/id/eprint/87344/ http://psasir.upm.edu.my/id/eprint/87344/ http://psasir.upm.edu.my/id/eprint/87344/1/Protective%20effects%20of%20stem%20cells%20from%20human%20exfoliated.pdf |