Promising discovery of benefcial Escherichia coli in the human gut
Ingested dietary fibres are hydrolysed by colon microbiota to produce energy-providing short-chain fatty acids (SCFA) that stimulate anti-inflammatory effects. SCFA-producing bacteria were screened from bacteria isolated from human faeces using bromothymol blue as an acid indicator and gas chromatog...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Springer
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/87336/ http://psasir.upm.edu.my/id/eprint/87336/1/Promising%20discovery%20of%20benefcial%20Escherichia%20coli%20in%20the%20human%20gut.pdf |
| _version_ | 1848860419379167232 |
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| author | Nakkarch, Atchareeya Foo, Hooi Ling Song, Adelene Ai Lian Nitisinprasert, Sunee Withayagiat, Ulaiwan |
| author_facet | Nakkarch, Atchareeya Foo, Hooi Ling Song, Adelene Ai Lian Nitisinprasert, Sunee Withayagiat, Ulaiwan |
| author_sort | Nakkarch, Atchareeya |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Ingested dietary fibres are hydrolysed by colon microbiota to produce energy-providing short-chain fatty acids (SCFA) that stimulate anti-inflammatory effects. SCFA-producing bacteria were screened from bacteria isolated from human faeces using bromothymol blue as an acid indicator and gas chromatography for SCFA profiling. The beneficial functions (antagonistic activity, haemolytic activities, antibiotic susceptibility, mucus adherent percentage and toxin gene detection) were evaluated for the top five SCFA-producing bacteria isolated from three healthy volunteers that identified as Escherichia coli strains. They produced acetic, propionic, isobutyric, butyric, isovaleric, valeric and caproic acids at average concentrations of 15.9, 1.8, 1.1, 1.9, 1.8, 2.7 and 3.4 mM, respectively. The SCFA production by E. coli strains was rapidly increased during the first 8 h of incubation and gradually decreased after 16 h of incubation. All E. coli strains showed acid and bile tolerance, resulting in a survival rate greater than 70% with no haemolytic activity, mucus adherence greater than 40% and susceptibility to conventional antibiotics. Hence, the selected E. coli strains exhibited promising probiotic properties with neither enterotoxin nor LPS producibility was detected. The present results confirm the existence of friendly and harmless E. coli strains in human microbiota as potential probiotics. |
| first_indexed | 2025-11-15T12:44:56Z |
| format | Article |
| id | upm-87336 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:44:56Z |
| publishDate | 2020 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-873362022-09-05T04:18:28Z http://psasir.upm.edu.my/id/eprint/87336/ Promising discovery of benefcial Escherichia coli in the human gut Nakkarch, Atchareeya Foo, Hooi Ling Song, Adelene Ai Lian Nitisinprasert, Sunee Withayagiat, Ulaiwan Ingested dietary fibres are hydrolysed by colon microbiota to produce energy-providing short-chain fatty acids (SCFA) that stimulate anti-inflammatory effects. SCFA-producing bacteria were screened from bacteria isolated from human faeces using bromothymol blue as an acid indicator and gas chromatography for SCFA profiling. The beneficial functions (antagonistic activity, haemolytic activities, antibiotic susceptibility, mucus adherent percentage and toxin gene detection) were evaluated for the top five SCFA-producing bacteria isolated from three healthy volunteers that identified as Escherichia coli strains. They produced acetic, propionic, isobutyric, butyric, isovaleric, valeric and caproic acids at average concentrations of 15.9, 1.8, 1.1, 1.9, 1.8, 2.7 and 3.4 mM, respectively. The SCFA production by E. coli strains was rapidly increased during the first 8 h of incubation and gradually decreased after 16 h of incubation. All E. coli strains showed acid and bile tolerance, resulting in a survival rate greater than 70% with no haemolytic activity, mucus adherence greater than 40% and susceptibility to conventional antibiotics. Hence, the selected E. coli strains exhibited promising probiotic properties with neither enterotoxin nor LPS producibility was detected. The present results confirm the existence of friendly and harmless E. coli strains in human microbiota as potential probiotics. Springer 2020-06-09 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/87336/1/Promising%20discovery%20of%20benefcial%20Escherichia%20coli%20in%20the%20human%20gut.pdf Nakkarch, Atchareeya and Foo, Hooi Ling and Song, Adelene Ai Lian and Nitisinprasert, Sunee and Withayagiat, Ulaiwan (2020) Promising discovery of benefcial Escherichia coli in the human gut. 3 Biotech, 10 (7). art. no. 296. pp. 1-14. ISSN 2190-572X; ESSN: 2190-5738 https://link.springer.com/article/10.1007/s13205-020-02289-z 10.1007/s13205-020-02289-z |
| spellingShingle | Nakkarch, Atchareeya Foo, Hooi Ling Song, Adelene Ai Lian Nitisinprasert, Sunee Withayagiat, Ulaiwan Promising discovery of benefcial Escherichia coli in the human gut |
| title | Promising discovery of benefcial Escherichia coli in the human gut |
| title_full | Promising discovery of benefcial Escherichia coli in the human gut |
| title_fullStr | Promising discovery of benefcial Escherichia coli in the human gut |
| title_full_unstemmed | Promising discovery of benefcial Escherichia coli in the human gut |
| title_short | Promising discovery of benefcial Escherichia coli in the human gut |
| title_sort | promising discovery of benefcial escherichia coli in the human gut |
| url | http://psasir.upm.edu.my/id/eprint/87336/ http://psasir.upm.edu.my/id/eprint/87336/ http://psasir.upm.edu.my/id/eprint/87336/ http://psasir.upm.edu.my/id/eprint/87336/1/Promising%20discovery%20of%20benefcial%20Escherichia%20coli%20in%20the%20human%20gut.pdf |