Phospholipid-protein structured membrane for microencapsulation of DHA oil and evaluation of its in vitro digestibility: inspired by milk fat globule membrane
The present study aims to design a milk fat globule membrane (MFGM)-inspired structured membrane (phospholipid- and protein-rich) for microencapsulation of docosahexaenoic acid (DHA) oil. DHA-enriched oil emulsions were prepared using different ratios of sunflower phospholipid (SPL), proteins [whey...
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| Format: | Article |
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American Chemical Society (ACS)
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/87152/ |
| _version_ | 1848860373840560128 |
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| author | Oi, Ming Lai Chin-Ping Tan, Ping Tan Ling, Zhi Cheong Chen, Ying Ge, Hui Lai, Oi-Ming |
| author_facet | Oi, Ming Lai Chin-Ping Tan, Ping Tan Ling, Zhi Cheong Chen, Ying Ge, Hui Lai, Oi-Ming |
| author_sort | Oi, Ming Lai |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The present study aims to design a milk fat globule membrane (MFGM)-inspired structured membrane (phospholipid- and protein-rich) for microencapsulation of docosahexaenoic acid (DHA) oil. DHA-enriched oil emulsions were prepared using different ratios of sunflower phospholipid (SPL), proteins [whey protein concentrate (WPC), soy protein isolate (SPI), and sodium caseinate (SC)], and maltodextrin and spray-dried to obtain DHA microcapsules. The prepared DHA oil emulsions have nanosized particles. SPLs were found to affect the secondary structure of WPC, which resulted in increased exposure of the protein hydrophobic site and emulsion stability. SPL also reduced the surface tension and viscosity of the DHA oil emulsions. In vitro digestion of the spray-dried DHA microcapsules showed that they were able to effectively resist gastric proteolysis and protect their bioactivity en route to the intestine. The DHA microcapsules have a high lipid digestibility in the small intestine with a high DHA hydrolysis efficiency (74.3%), which is higher than that of commercial DHA microcapsules. |
| first_indexed | 2025-11-15T12:44:13Z |
| format | Article |
| id | upm-87152 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T12:44:13Z |
| publishDate | 2020 |
| publisher | American Chemical Society (ACS) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-871522024-05-17T04:27:37Z http://psasir.upm.edu.my/id/eprint/87152/ Phospholipid-protein structured membrane for microencapsulation of DHA oil and evaluation of its in vitro digestibility: inspired by milk fat globule membrane Oi, Ming Lai Chin-Ping Tan, Ping Tan Ling, Zhi Cheong Chen, Ying Ge, Hui Lai, Oi-Ming The present study aims to design a milk fat globule membrane (MFGM)-inspired structured membrane (phospholipid- and protein-rich) for microencapsulation of docosahexaenoic acid (DHA) oil. DHA-enriched oil emulsions were prepared using different ratios of sunflower phospholipid (SPL), proteins [whey protein concentrate (WPC), soy protein isolate (SPI), and sodium caseinate (SC)], and maltodextrin and spray-dried to obtain DHA microcapsules. The prepared DHA oil emulsions have nanosized particles. SPLs were found to affect the secondary structure of WPC, which resulted in increased exposure of the protein hydrophobic site and emulsion stability. SPL also reduced the surface tension and viscosity of the DHA oil emulsions. In vitro digestion of the spray-dried DHA microcapsules showed that they were able to effectively resist gastric proteolysis and protect their bioactivity en route to the intestine. The DHA microcapsules have a high lipid digestibility in the small intestine with a high DHA hydrolysis efficiency (74.3%), which is higher than that of commercial DHA microcapsules. American Chemical Society (ACS) 2020 Article PeerReviewed Oi, Ming Lai and Chin-Ping Tan, Ping Tan and Ling, Zhi Cheong and Chen, Ying and Ge, Hui and Lai, Oi-Ming (2020) Phospholipid-protein structured membrane for microencapsulation of DHA oil and evaluation of its in vitro digestibility: inspired by milk fat globule membrane. Journal of Agricultural And Food Chemistry, 68 (22). 6190 - 6201. ISSN 0021-8561; ESSN: 1520-5118 https://pubs.acs.org/doi/10.1021/acs.jafc.0c01250 10.1021/acs.jafc.0c01250 |
| spellingShingle | Oi, Ming Lai Chin-Ping Tan, Ping Tan Ling, Zhi Cheong Chen, Ying Ge, Hui Lai, Oi-Ming Phospholipid-protein structured membrane for microencapsulation of DHA oil and evaluation of its in vitro digestibility: inspired by milk fat globule membrane |
| title | Phospholipid-protein structured membrane for microencapsulation of DHA oil and evaluation of its in vitro digestibility: inspired by milk fat globule membrane |
| title_full | Phospholipid-protein structured membrane for microencapsulation of DHA oil and evaluation of its in vitro digestibility: inspired by milk fat globule membrane |
| title_fullStr | Phospholipid-protein structured membrane for microencapsulation of DHA oil and evaluation of its in vitro digestibility: inspired by milk fat globule membrane |
| title_full_unstemmed | Phospholipid-protein structured membrane for microencapsulation of DHA oil and evaluation of its in vitro digestibility: inspired by milk fat globule membrane |
| title_short | Phospholipid-protein structured membrane for microencapsulation of DHA oil and evaluation of its in vitro digestibility: inspired by milk fat globule membrane |
| title_sort | phospholipid-protein structured membrane for microencapsulation of dha oil and evaluation of its in vitro digestibility: inspired by milk fat globule membrane |
| url | http://psasir.upm.edu.my/id/eprint/87152/ http://psasir.upm.edu.my/id/eprint/87152/ http://psasir.upm.edu.my/id/eprint/87152/ |