Phenolic profiling and evaluation of in vitro antioxidant, α-glucosidase and α-amylase inhibitory activities of Lepisanthes fruticosa (Roxb) Leenh fruit extracts
The present study focused on the phytochemical profiling along with evaluation of in vitro antioxidant, α-glucosidase and α-amylase inhibitory activities of various crudes and fractions obtained from Lepisanthes fruticosa (Roxb) Leenh fruit. Ethanolic seed crude extract exhibited the strongest radic...
| Main Authors: | , , , , |
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| Format: | Article |
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Elsevier BV
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/87148/ |
| _version_ | 1848860372744798208 |
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| author | Hasan Salahuddin, Mirfat Ahmad Ismail, Amin Kassim, Nur Kartinee Hamid, Muhajir Mat Ali, Mohd Shukri |
| author_facet | Hasan Salahuddin, Mirfat Ahmad Ismail, Amin Kassim, Nur Kartinee Hamid, Muhajir Mat Ali, Mohd Shukri |
| author_sort | Hasan Salahuddin, Mirfat Ahmad |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The present study focused on the phytochemical profiling along with evaluation of in vitro antioxidant, α-glucosidase and α-amylase inhibitory activities of various crudes and fractions obtained from Lepisanthes fruticosa (Roxb) Leenh fruit. Ethanolic seed crude extract exhibited the strongest radical scavenging, β-carotene bleaching activity, α-glucosidase inhibition and the highest total phenolic content (TPC). Column chromatography afforded various fractions with fraction M4 being the most potent due to the strongest radical scavenging, β-carotene bleaching, α-glucosidase inhibition and greatest amount of TPC. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis of ethanolic seed crude extract and fraction M4 showed the presence of various phytochemicals with antioxidant and antidiabetic properties, which include mostly flavonoids and tannins. The results may suggest that the ethanolic crude seed extract and its fraction could be an excellent source of bioactive phytochemicals with antioxidant and antidiabetic potential. |
| first_indexed | 2025-11-15T12:44:12Z |
| format | Article |
| id | upm-87148 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T12:44:12Z |
| publishDate | 2020 |
| publisher | Elsevier BV |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-871482024-05-17T04:10:52Z http://psasir.upm.edu.my/id/eprint/87148/ Phenolic profiling and evaluation of in vitro antioxidant, α-glucosidase and α-amylase inhibitory activities of Lepisanthes fruticosa (Roxb) Leenh fruit extracts Hasan Salahuddin, Mirfat Ahmad Ismail, Amin Kassim, Nur Kartinee Hamid, Muhajir Mat Ali, Mohd Shukri The present study focused on the phytochemical profiling along with evaluation of in vitro antioxidant, α-glucosidase and α-amylase inhibitory activities of various crudes and fractions obtained from Lepisanthes fruticosa (Roxb) Leenh fruit. Ethanolic seed crude extract exhibited the strongest radical scavenging, β-carotene bleaching activity, α-glucosidase inhibition and the highest total phenolic content (TPC). Column chromatography afforded various fractions with fraction M4 being the most potent due to the strongest radical scavenging, β-carotene bleaching, α-glucosidase inhibition and greatest amount of TPC. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis of ethanolic seed crude extract and fraction M4 showed the presence of various phytochemicals with antioxidant and antidiabetic properties, which include mostly flavonoids and tannins. The results may suggest that the ethanolic crude seed extract and its fraction could be an excellent source of bioactive phytochemicals with antioxidant and antidiabetic potential. Elsevier BV 2020 Article PeerReviewed Hasan Salahuddin, Mirfat Ahmad and Ismail, Amin and Kassim, Nur Kartinee and Hamid, Muhajir and Mat Ali, Mohd Shukri (2020) Phenolic profiling and evaluation of in vitro antioxidant, α-glucosidase and α-amylase inhibitory activities of Lepisanthes fruticosa (Roxb) Leenh fruit extracts. Food Chemistry, 331. art. no. 127240. pp. 1-10. ISSN 0308-8146 https://linkinghub.elsevier.com/retrieve/pii/S030881462031102X 10.1016/j.foodchem.2020.127240 |
| spellingShingle | Hasan Salahuddin, Mirfat Ahmad Ismail, Amin Kassim, Nur Kartinee Hamid, Muhajir Mat Ali, Mohd Shukri Phenolic profiling and evaluation of in vitro antioxidant, α-glucosidase and α-amylase inhibitory activities of Lepisanthes fruticosa (Roxb) Leenh fruit extracts |
| title | Phenolic profiling and evaluation of in vitro antioxidant, α-glucosidase and α-amylase inhibitory activities of Lepisanthes fruticosa (Roxb) Leenh fruit extracts |
| title_full | Phenolic profiling and evaluation of in vitro antioxidant, α-glucosidase and α-amylase inhibitory activities of Lepisanthes fruticosa (Roxb) Leenh fruit extracts |
| title_fullStr | Phenolic profiling and evaluation of in vitro antioxidant, α-glucosidase and α-amylase inhibitory activities of Lepisanthes fruticosa (Roxb) Leenh fruit extracts |
| title_full_unstemmed | Phenolic profiling and evaluation of in vitro antioxidant, α-glucosidase and α-amylase inhibitory activities of Lepisanthes fruticosa (Roxb) Leenh fruit extracts |
| title_short | Phenolic profiling and evaluation of in vitro antioxidant, α-glucosidase and α-amylase inhibitory activities of Lepisanthes fruticosa (Roxb) Leenh fruit extracts |
| title_sort | phenolic profiling and evaluation of in vitro antioxidant, α-glucosidase and α-amylase inhibitory activities of lepisanthes fruticosa (roxb) leenh fruit extracts |
| url | http://psasir.upm.edu.my/id/eprint/87148/ http://psasir.upm.edu.my/id/eprint/87148/ http://psasir.upm.edu.my/id/eprint/87148/ |