Peroxiredoxin of Arthrospira platensis derived short molecule YT12 influences antioxidant and anticancer activity
This study demonstrates both the antioxidant and anticancer potential of the novel short molecule YT12 derived from peroxiredoxin (Prx) of spirulina, Arthrospira platensis (Ap). ApPrx showed significant reduction in reactive oxygen species (ROS) against hydrogen peroxide (H2O2) stress. The complemen...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2020
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/87127/ http://psasir.upm.edu.my/id/eprint/87127/1/Peroxiredoxin%20of%20Arthrospira%20platensis%20derived%20short%20molecule%20YT12.pdf |
| _version_ | 1848860367589998592 |
|---|---|
| author | Sannasimuthu, Anbazahan Ramani, Madhura Pasupuleti, Mukesh Saraswathi, Nambiappan T. Arasu, Mariadhas Valan Al-Dhabi, Naif Abdullah Arshad, Aziz Mala, Kanchana Arockiaraj, Jesu |
| author_facet | Sannasimuthu, Anbazahan Ramani, Madhura Pasupuleti, Mukesh Saraswathi, Nambiappan T. Arasu, Mariadhas Valan Al-Dhabi, Naif Abdullah Arshad, Aziz Mala, Kanchana Arockiaraj, Jesu |
| author_sort | Sannasimuthu, Anbazahan |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | This study demonstrates both the antioxidant and anticancer potential of the novel short molecule YT12 derived from peroxiredoxin (Prx) of spirulina, Arthrospira platensis (Ap). ApPrx showed significant reduction in reactive oxygen species (ROS) against hydrogen peroxide (H2O2) stress. The complementary DNA sequence of ApPrx contained 706 nucleotides and its coding region possessed 546 nucleotides between position 115 and 660. Real-time quantitative reverse transcription polymerase chain reaction analysis confirmed the messenger RNA expression of ApPrx due to H2O2 exposure in spirulina cells at regular intervals, in which the highest expression was noticed on Day 20. Cytotoxicity assay was performed using human peripheral blood mononuclear cells, and revealed that at 10 μM, the YT12 did not exhibit any notable toxicity. Furthermore, ROS scavenging activity of YT12 was performed using DCF-DA assay, in which YT12 scavenged a significant amount of ROS at 25 μM in H2O2-treated blood leukocytes. The intracellular ROS in human colon adenocarcinoma cells (HT-29) was regulated by oxidative stress, where the YT12 scavenges ROS in HT-29 cells at 12.5 μM. Findings show that YT12 peptide has anticancer activity, when treated against HT-29 cells. Through the MTT assay, YT12 showed vital cytotoxicity against HT-29 cells. These finding suggested that YT12 is a potent antioxidant molecule which defends ROS against oxidative stress and plays a role in redox balance. |
| first_indexed | 2025-11-15T12:44:07Z |
| format | Article |
| id | upm-87127 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:44:07Z |
| publishDate | 2020 |
| publisher | Wiley |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-871272021-11-22T02:01:07Z http://psasir.upm.edu.my/id/eprint/87127/ Peroxiredoxin of Arthrospira platensis derived short molecule YT12 influences antioxidant and anticancer activity Sannasimuthu, Anbazahan Ramani, Madhura Pasupuleti, Mukesh Saraswathi, Nambiappan T. Arasu, Mariadhas Valan Al-Dhabi, Naif Abdullah Arshad, Aziz Mala, Kanchana Arockiaraj, Jesu This study demonstrates both the antioxidant and anticancer potential of the novel short molecule YT12 derived from peroxiredoxin (Prx) of spirulina, Arthrospira platensis (Ap). ApPrx showed significant reduction in reactive oxygen species (ROS) against hydrogen peroxide (H2O2) stress. The complementary DNA sequence of ApPrx contained 706 nucleotides and its coding region possessed 546 nucleotides between position 115 and 660. Real-time quantitative reverse transcription polymerase chain reaction analysis confirmed the messenger RNA expression of ApPrx due to H2O2 exposure in spirulina cells at regular intervals, in which the highest expression was noticed on Day 20. Cytotoxicity assay was performed using human peripheral blood mononuclear cells, and revealed that at 10 μM, the YT12 did not exhibit any notable toxicity. Furthermore, ROS scavenging activity of YT12 was performed using DCF-DA assay, in which YT12 scavenged a significant amount of ROS at 25 μM in H2O2-treated blood leukocytes. The intracellular ROS in human colon adenocarcinoma cells (HT-29) was regulated by oxidative stress, where the YT12 scavenges ROS in HT-29 cells at 12.5 μM. Findings show that YT12 peptide has anticancer activity, when treated against HT-29 cells. Through the MTT assay, YT12 showed vital cytotoxicity against HT-29 cells. These finding suggested that YT12 is a potent antioxidant molecule which defends ROS against oxidative stress and plays a role in redox balance. Wiley 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/87127/1/Peroxiredoxin%20of%20Arthrospira%20platensis%20derived%20short%20molecule%20YT12.pdf Sannasimuthu, Anbazahan and Ramani, Madhura and Pasupuleti, Mukesh and Saraswathi, Nambiappan T. and Arasu, Mariadhas Valan and Al-Dhabi, Naif Abdullah and Arshad, Aziz and Mala, Kanchana and Arockiaraj, Jesu (2020) Peroxiredoxin of Arthrospira platensis derived short molecule YT12 influences antioxidant and anticancer activity. Cell Biology International, 44 (11). 2231 - 2242. ISSN 1065-6995; ESSN: 1095-8355 https://onlinelibrary.wiley.com/doi/abs/10.1002/cbin.11431 10.1002/cbin.11431 |
| spellingShingle | Sannasimuthu, Anbazahan Ramani, Madhura Pasupuleti, Mukesh Saraswathi, Nambiappan T. Arasu, Mariadhas Valan Al-Dhabi, Naif Abdullah Arshad, Aziz Mala, Kanchana Arockiaraj, Jesu Peroxiredoxin of Arthrospira platensis derived short molecule YT12 influences antioxidant and anticancer activity |
| title | Peroxiredoxin of Arthrospira platensis derived short molecule YT12 influences antioxidant and anticancer activity |
| title_full | Peroxiredoxin of Arthrospira platensis derived short molecule YT12 influences antioxidant and anticancer activity |
| title_fullStr | Peroxiredoxin of Arthrospira platensis derived short molecule YT12 influences antioxidant and anticancer activity |
| title_full_unstemmed | Peroxiredoxin of Arthrospira platensis derived short molecule YT12 influences antioxidant and anticancer activity |
| title_short | Peroxiredoxin of Arthrospira platensis derived short molecule YT12 influences antioxidant and anticancer activity |
| title_sort | peroxiredoxin of arthrospira platensis derived short molecule yt12 influences antioxidant and anticancer activity |
| url | http://psasir.upm.edu.my/id/eprint/87127/ http://psasir.upm.edu.my/id/eprint/87127/ http://psasir.upm.edu.my/id/eprint/87127/ http://psasir.upm.edu.my/id/eprint/87127/1/Peroxiredoxin%20of%20Arthrospira%20platensis%20derived%20short%20molecule%20YT12.pdf |