Optimization of protective agents for the freeze-drying of Paenibacillus polymyxa Kp10 as a potential biofungicide
Anthracnose is a fungal disease causing major losses in crop production. Chemical fungicides widely used in crop plantations to combat fungal infections can be a threat to the environment and humans in the long term. Recently, biofungicides have gained much interest as an alternative to chemical fun...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Multidisciplinary Digital Publishing Institute
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/87011/ http://psasir.upm.edu.my/id/eprint/87011/1/Optimization%20of%20protective%20agents%20for%20the.pdf |
| _version_ | 1848860343497916416 |
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| author | Nasran, Hayatun Syamila Mohd. Yusof, Hidayat Halim, Murni Abdul Rahman, Nor’Aini |
| author_facet | Nasran, Hayatun Syamila Mohd. Yusof, Hidayat Halim, Murni Abdul Rahman, Nor’Aini |
| author_sort | Nasran, Hayatun Syamila |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Anthracnose is a fungal disease causing major losses in crop production. Chemical fungicides widely used in crop plantations to combat fungal infections can be a threat to the environment and humans in the long term. Recently, biofungicides have gained much interest as an alternative to chemical fungicides due to their environmentally friendly nature. Biofungicide products in powder form can be formulated using the freeze-drying technique to provide convenient storage. Protective agent formulation is needed in maintaining the optimal viable cells of biofungicide products. In this study, 8.10 log colony-forming unit (CFU)/mL was the highest cell viability of Paenibacillus polymyxa Kp10 at 22 h during incubation. The effects of several selected protective agents on the viability of P. polymyxa Kp10 after freeze-drying were studied. Response surface methodology (RSM) was used for optimizing formulation for the protective agents. The combination of lactose (10% w/v), skim milk (20% w/v), and sucrose (27.5% w/v) was found to be suitable for preserving P. polymyxa Kp10 during freeze-drying. Further, P. polymyxa Kp10 demonstrated the ability to inhibit fungal pathogens, Colletotrichum truncatum and C. gloeosporioides, at 60.18% and 66.52% of inhibition of radial growth, respectively. |
| first_indexed | 2025-11-15T12:43:44Z |
| format | Article |
| id | upm-87011 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:43:44Z |
| publishDate | 2020 |
| publisher | Multidisciplinary Digital Publishing Institute |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-870112022-01-10T08:17:22Z http://psasir.upm.edu.my/id/eprint/87011/ Optimization of protective agents for the freeze-drying of Paenibacillus polymyxa Kp10 as a potential biofungicide Nasran, Hayatun Syamila Mohd. Yusof, Hidayat Halim, Murni Abdul Rahman, Nor’Aini Anthracnose is a fungal disease causing major losses in crop production. Chemical fungicides widely used in crop plantations to combat fungal infections can be a threat to the environment and humans in the long term. Recently, biofungicides have gained much interest as an alternative to chemical fungicides due to their environmentally friendly nature. Biofungicide products in powder form can be formulated using the freeze-drying technique to provide convenient storage. Protective agent formulation is needed in maintaining the optimal viable cells of biofungicide products. In this study, 8.10 log colony-forming unit (CFU)/mL was the highest cell viability of Paenibacillus polymyxa Kp10 at 22 h during incubation. The effects of several selected protective agents on the viability of P. polymyxa Kp10 after freeze-drying were studied. Response surface methodology (RSM) was used for optimizing formulation for the protective agents. The combination of lactose (10% w/v), skim milk (20% w/v), and sucrose (27.5% w/v) was found to be suitable for preserving P. polymyxa Kp10 during freeze-drying. Further, P. polymyxa Kp10 demonstrated the ability to inhibit fungal pathogens, Colletotrichum truncatum and C. gloeosporioides, at 60.18% and 66.52% of inhibition of radial growth, respectively. Multidisciplinary Digital Publishing Institute 2020-06-04 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/87011/1/Optimization%20of%20protective%20agents%20for%20the.pdf Nasran, Hayatun Syamila and Mohd. Yusof, Hidayat and Halim, Murni and Abdul Rahman, Nor’Aini (2020) Optimization of protective agents for the freeze-drying of Paenibacillus polymyxa Kp10 as a potential biofungicide. Molecules, 25 (11). pp. 1-11. ISSN 1420-3049 https://www.mdpi.com/1420-3049/25/11/2618 10.3390/molecules25112618 |
| spellingShingle | Nasran, Hayatun Syamila Mohd. Yusof, Hidayat Halim, Murni Abdul Rahman, Nor’Aini Optimization of protective agents for the freeze-drying of Paenibacillus polymyxa Kp10 as a potential biofungicide |
| title | Optimization of protective agents for the freeze-drying of Paenibacillus polymyxa Kp10 as a potential biofungicide |
| title_full | Optimization of protective agents for the freeze-drying of Paenibacillus polymyxa Kp10 as a potential biofungicide |
| title_fullStr | Optimization of protective agents for the freeze-drying of Paenibacillus polymyxa Kp10 as a potential biofungicide |
| title_full_unstemmed | Optimization of protective agents for the freeze-drying of Paenibacillus polymyxa Kp10 as a potential biofungicide |
| title_short | Optimization of protective agents for the freeze-drying of Paenibacillus polymyxa Kp10 as a potential biofungicide |
| title_sort | optimization of protective agents for the freeze-drying of paenibacillus polymyxa kp10 as a potential biofungicide |
| url | http://psasir.upm.edu.my/id/eprint/87011/ http://psasir.upm.edu.my/id/eprint/87011/ http://psasir.upm.edu.my/id/eprint/87011/ http://psasir.upm.edu.my/id/eprint/87011/1/Optimization%20of%20protective%20agents%20for%20the.pdf |