Optimization of phenol degradation by Antarctic bacterium Rhodococcus sp.
This study focused on the ability of the Antarctic bacterium Rhodococcus sp. strain AQ5-14 to survive exposure to and to degrade high concentrations of phenol at 0.5 g l-1. After initial evaluation of phenol-degrading performance, the effects of salinity, pH and temperature on the rate of phenol deg...
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| Format: | Article |
| Language: | English |
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Cambridge University Press
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/87009/ http://psasir.upm.edu.my/id/eprint/87009/1/Optimization%20of%20phenol%20degradation%20by%20Antarctic%20bacterium%20Rhodococcus%20sp.pdf |
| _version_ | 1848860342859333632 |
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| author | Tengku Marzuki, Tengku Athirrah Subramanian, Kavilasni Zakaria, Nur Nadhirah Convey, Peter Abdul Khalil, Khalilah Lee, Gillian Li Yin Zulkharnain, Azham Shaharuddin, Noor Azmi Ahmad, Siti Aqlima |
| author_facet | Tengku Marzuki, Tengku Athirrah Subramanian, Kavilasni Zakaria, Nur Nadhirah Convey, Peter Abdul Khalil, Khalilah Lee, Gillian Li Yin Zulkharnain, Azham Shaharuddin, Noor Azmi Ahmad, Siti Aqlima |
| author_sort | Tengku Marzuki, Tengku Athirrah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | This study focused on the ability of the Antarctic bacterium Rhodococcus sp. strain AQ5-14 to survive exposure to and to degrade high concentrations of phenol at 0.5 g l-1. After initial evaluation of phenol-degrading performance, the effects of salinity, pH and temperature on the rate of phenol degradation were examined. The optimum conditions for phenol degradation were pH 7 and 0.4 g l-1 NaCl at a temperature of 25°C (83.90%). An analysis using response surface methodology (RSM) and the Plackett-Burman design identified salinity, pH and temperature as three statistically significant factors influencing phenol degradation. The maximum bacterial growth was observed (optical density at 600 nm = 0.455), with medium conditions of pH 6.5, 22.5°C and 0.47 g l-1 NaCl in the central composite design of the RSM experiments enhancing phenol degradation to 99.10%. A central composite design was then used to examine the interactions among these three variables and to determine their optimal levels. There was excellent agreement (R2 = 0.9785) between experimental and predicted values, with less strong but still good agreement (R2 = 0.8376) between the predicted model values and those obtained experimentally under optimized conditions. Rhodococcus sp. strain AQ5-14 has excellent potential for the bioremediation of phenol. |
| first_indexed | 2025-11-15T12:43:43Z |
| format | Article |
| id | upm-87009 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:43:43Z |
| publishDate | 2020 |
| publisher | Cambridge University Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-870092022-01-10T08:31:11Z http://psasir.upm.edu.my/id/eprint/87009/ Optimization of phenol degradation by Antarctic bacterium Rhodococcus sp. Tengku Marzuki, Tengku Athirrah Subramanian, Kavilasni Zakaria, Nur Nadhirah Convey, Peter Abdul Khalil, Khalilah Lee, Gillian Li Yin Zulkharnain, Azham Shaharuddin, Noor Azmi Ahmad, Siti Aqlima This study focused on the ability of the Antarctic bacterium Rhodococcus sp. strain AQ5-14 to survive exposure to and to degrade high concentrations of phenol at 0.5 g l-1. After initial evaluation of phenol-degrading performance, the effects of salinity, pH and temperature on the rate of phenol degradation were examined. The optimum conditions for phenol degradation were pH 7 and 0.4 g l-1 NaCl at a temperature of 25°C (83.90%). An analysis using response surface methodology (RSM) and the Plackett-Burman design identified salinity, pH and temperature as three statistically significant factors influencing phenol degradation. The maximum bacterial growth was observed (optical density at 600 nm = 0.455), with medium conditions of pH 6.5, 22.5°C and 0.47 g l-1 NaCl in the central composite design of the RSM experiments enhancing phenol degradation to 99.10%. A central composite design was then used to examine the interactions among these three variables and to determine their optimal levels. There was excellent agreement (R2 = 0.9785) between experimental and predicted values, with less strong but still good agreement (R2 = 0.8376) between the predicted model values and those obtained experimentally under optimized conditions. Rhodococcus sp. strain AQ5-14 has excellent potential for the bioremediation of phenol. Cambridge University Press 2020-07-06 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/87009/1/Optimization%20of%20phenol%20degradation%20by%20Antarctic%20bacterium%20Rhodococcus%20sp.pdf Tengku Marzuki, Tengku Athirrah and Subramanian, Kavilasni and Zakaria, Nur Nadhirah and Convey, Peter and Abdul Khalil, Khalilah and Lee, Gillian Li Yin and Zulkharnain, Azham and Shaharuddin, Noor Azmi and Ahmad, Siti Aqlima (2020) Optimization of phenol degradation by Antarctic bacterium Rhodococcus sp. Antarctic Science, 32 (6). pp. 486-495. ISSN 0954-1020; ESSN: 1365-2079 https://www.cambridge.org/core/journals/antarctic-science/article/abs/optimization-of-phenol-degradation-by-antarctic-bacterium-rhodococcus-sp/F8D5DF954D20817694C3012369651D34 10.1017/S0954102020000358 |
| spellingShingle | Tengku Marzuki, Tengku Athirrah Subramanian, Kavilasni Zakaria, Nur Nadhirah Convey, Peter Abdul Khalil, Khalilah Lee, Gillian Li Yin Zulkharnain, Azham Shaharuddin, Noor Azmi Ahmad, Siti Aqlima Optimization of phenol degradation by Antarctic bacterium Rhodococcus sp. |
| title | Optimization of phenol degradation by Antarctic bacterium Rhodococcus sp. |
| title_full | Optimization of phenol degradation by Antarctic bacterium Rhodococcus sp. |
| title_fullStr | Optimization of phenol degradation by Antarctic bacterium Rhodococcus sp. |
| title_full_unstemmed | Optimization of phenol degradation by Antarctic bacterium Rhodococcus sp. |
| title_short | Optimization of phenol degradation by Antarctic bacterium Rhodococcus sp. |
| title_sort | optimization of phenol degradation by antarctic bacterium rhodococcus sp. |
| url | http://psasir.upm.edu.my/id/eprint/87009/ http://psasir.upm.edu.my/id/eprint/87009/ http://psasir.upm.edu.my/id/eprint/87009/ http://psasir.upm.edu.my/id/eprint/87009/1/Optimization%20of%20phenol%20degradation%20by%20Antarctic%20bacterium%20Rhodococcus%20sp.pdf |