Novel fructooligasoccharide conversion from sugarcane syrup using a specialised enzymatic pH-stat bioreactor
Fructooligosaccharides (FOS) have gained significant attention for their prebiotic properties. Given that sugarcane syrup (SS) is sucrose-rich but with other nutritional benefits, its direct transformation into FOS may add value to this product. Therefore, the aims of this study were to develop FOS...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/86925/ http://psasir.upm.edu.my/id/eprint/86925/1/Novel%20fructooligasoccharide.pdf |
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| author | Azhari, Siti Hajar Abd Rahim, Muhamad Hafiz Wan Mohtar, Wan Abd Al Qadr Imad Sarbini, Shahrul Razid Saari, Nazamid |
| author_facet | Azhari, Siti Hajar Abd Rahim, Muhamad Hafiz Wan Mohtar, Wan Abd Al Qadr Imad Sarbini, Shahrul Razid Saari, Nazamid |
| author_sort | Azhari, Siti Hajar |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Fructooligosaccharides (FOS) have gained significant attention for their prebiotic properties. Given that sugarcane syrup (SS) is sucrose-rich but with other nutritional benefits, its direct transformation into FOS may add value to this product. Therefore, the aims of this study were to develop FOS conversion from SS and to define the kinetic behaviour of FOS synthesized in a 1-L specialized pH-stat bioreactor (SPSB). The SS was composed of sucrose (58.93%) with considerable antioxidant capacities and Ƴ-aminobutyric acid. The developed SPSB process consisted of three stages: evaporation of sugarcane juice into syrup (68–75 °Brix) (stage 1), optimization of the Viscozyme L and SS mixture at different reaction temperatures (47–55 °C) (stage 2), and upscaling of the optimized reaction system under defined conditions in a 1 L-SPSB system (stage 3). In the 1 L-SPSB system, the enzymatic reaction yielded 32.22% of FOS from SS after a 6 h reaction, which is comparable with a pure system containing an equivalent concentration of 10% of sucrose as initial substrate with 39.55% yield. This result demonstrated the efficient conversion of SS into FOS, supporting the utilization of sugarcane juice for its health benefits. |
| first_indexed | 2025-11-15T12:43:24Z |
| format | Article |
| id | upm-86925 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:43:24Z |
| publishDate | 2020 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-869252022-01-05T08:30:25Z http://psasir.upm.edu.my/id/eprint/86925/ Novel fructooligasoccharide conversion from sugarcane syrup using a specialised enzymatic pH-stat bioreactor Azhari, Siti Hajar Abd Rahim, Muhamad Hafiz Wan Mohtar, Wan Abd Al Qadr Imad Sarbini, Shahrul Razid Saari, Nazamid Fructooligosaccharides (FOS) have gained significant attention for their prebiotic properties. Given that sugarcane syrup (SS) is sucrose-rich but with other nutritional benefits, its direct transformation into FOS may add value to this product. Therefore, the aims of this study were to develop FOS conversion from SS and to define the kinetic behaviour of FOS synthesized in a 1-L specialized pH-stat bioreactor (SPSB). The SS was composed of sucrose (58.93%) with considerable antioxidant capacities and Ƴ-aminobutyric acid. The developed SPSB process consisted of three stages: evaporation of sugarcane juice into syrup (68–75 °Brix) (stage 1), optimization of the Viscozyme L and SS mixture at different reaction temperatures (47–55 °C) (stage 2), and upscaling of the optimized reaction system under defined conditions in a 1 L-SPSB system (stage 3). In the 1 L-SPSB system, the enzymatic reaction yielded 32.22% of FOS from SS after a 6 h reaction, which is comparable with a pure system containing an equivalent concentration of 10% of sucrose as initial substrate with 39.55% yield. This result demonstrated the efficient conversion of SS into FOS, supporting the utilization of sugarcane juice for its health benefits. Elsevier 2020-08 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/86925/1/Novel%20fructooligasoccharide.pdf Azhari, Siti Hajar and Abd Rahim, Muhamad Hafiz and Wan Mohtar, Wan Abd Al Qadr Imad and Sarbini, Shahrul Razid and Saari, Nazamid (2020) Novel fructooligasoccharide conversion from sugarcane syrup using a specialised enzymatic pH-stat bioreactor. Process Biochemistry, 95. 55 - 63. ISSN 1359-5113; ESSN: 1873-3298 https://www.sciencedirect.com/science/article/abs/pii/S135951132030283X 10.1016/j.procbio.2020.04.031 |
| spellingShingle | Azhari, Siti Hajar Abd Rahim, Muhamad Hafiz Wan Mohtar, Wan Abd Al Qadr Imad Sarbini, Shahrul Razid Saari, Nazamid Novel fructooligasoccharide conversion from sugarcane syrup using a specialised enzymatic pH-stat bioreactor |
| title | Novel fructooligasoccharide conversion from sugarcane syrup using a specialised enzymatic pH-stat bioreactor |
| title_full | Novel fructooligasoccharide conversion from sugarcane syrup using a specialised enzymatic pH-stat bioreactor |
| title_fullStr | Novel fructooligasoccharide conversion from sugarcane syrup using a specialised enzymatic pH-stat bioreactor |
| title_full_unstemmed | Novel fructooligasoccharide conversion from sugarcane syrup using a specialised enzymatic pH-stat bioreactor |
| title_short | Novel fructooligasoccharide conversion from sugarcane syrup using a specialised enzymatic pH-stat bioreactor |
| title_sort | novel fructooligasoccharide conversion from sugarcane syrup using a specialised enzymatic ph-stat bioreactor |
| url | http://psasir.upm.edu.my/id/eprint/86925/ http://psasir.upm.edu.my/id/eprint/86925/ http://psasir.upm.edu.my/id/eprint/86925/ http://psasir.upm.edu.my/id/eprint/86925/1/Novel%20fructooligasoccharide.pdf |