NMR-based metabolomics reveals effect of Ganoderma boninense infection on oil palm leaf at 30 days post-infection
Basal stem rot is the major disease in oil palm industry that caused by a fungal named Ganoderma boninense (G. boninense) species. Infected palms are symptomless at the early stage of this disease which imposes difficulties in detecting the disease. Therefore, this study was carried out to obtain th...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Southern Cross Publishing
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/86908/ http://psasir.upm.edu.my/id/eprint/86908/1/NMR-based%20metabolomics%20reveals.pdf |
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| author | Isha, Azizul Yusof, Nor Azah Abdullah, Siti Nor Akmar Wong, Mui Yun Osman, Rosiah |
| author_facet | Isha, Azizul Yusof, Nor Azah Abdullah, Siti Nor Akmar Wong, Mui Yun Osman, Rosiah |
| author_sort | Isha, Azizul |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Basal stem rot is the major disease in oil palm industry that caused by a fungal named Ganoderma boninense (G. boninense) species. Infected palms are symptomless at the early stage of this disease which imposes difficulties in detecting the disease. Therefore, this study was carried out to obtain the 1 H NMR metabolomic profiling of both non-infected and G. boninense infected oil palm leaf at 30 days post-infection (dpi). This combination has provided a rapid approach in investigating the changes in the compound variations of non-infected and G. boninense infected oil palm leaf. Non-infected and G. boninense infected oil palm leaf at 30 dpi was extracted using aqueous methanol (methanol: water, 80: 20 v/v). The crude extracts obtained were analyzed by 1 H NMR-based metabolomics approach. Analysis of metabolomics data from 1 H NMR was conducted by multivariate data analysis of principal component analysis (PCA). Significant differences were found between the two groups. Compared to the non-infected leaf, the G. boninense infected leaf had higher relative abundance of choline, asparagine, alanine, succinic acid, gallic acid, epicatechin, trimethylamine, N-acetylglucosamine, N-acetyltyrosine, β-sitosterol, 2,3-butanediol, lactic acid, caffeic acid, phydroxybenzoic acid, α-tocopherol, β-cryptoxanthin and kaempferol. The non-infected leaf showed higher level of sucrose, xylose, α-glucose, S-sulfocysteine and indole-3-acetic acid. NMR-based metabolomics applied in this study reveals that G. boninense alters a manifold of primary and secondary compounds in oil palm leaf. |
| first_indexed | 2025-11-15T12:43:20Z |
| format | Article |
| id | upm-86908 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:43:20Z |
| publishDate | 2020 |
| publisher | Southern Cross Publishing |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-869082021-12-30T02:39:58Z http://psasir.upm.edu.my/id/eprint/86908/ NMR-based metabolomics reveals effect of Ganoderma boninense infection on oil palm leaf at 30 days post-infection Isha, Azizul Yusof, Nor Azah Abdullah, Siti Nor Akmar Wong, Mui Yun Osman, Rosiah Basal stem rot is the major disease in oil palm industry that caused by a fungal named Ganoderma boninense (G. boninense) species. Infected palms are symptomless at the early stage of this disease which imposes difficulties in detecting the disease. Therefore, this study was carried out to obtain the 1 H NMR metabolomic profiling of both non-infected and G. boninense infected oil palm leaf at 30 days post-infection (dpi). This combination has provided a rapid approach in investigating the changes in the compound variations of non-infected and G. boninense infected oil palm leaf. Non-infected and G. boninense infected oil palm leaf at 30 dpi was extracted using aqueous methanol (methanol: water, 80: 20 v/v). The crude extracts obtained were analyzed by 1 H NMR-based metabolomics approach. Analysis of metabolomics data from 1 H NMR was conducted by multivariate data analysis of principal component analysis (PCA). Significant differences were found between the two groups. Compared to the non-infected leaf, the G. boninense infected leaf had higher relative abundance of choline, asparagine, alanine, succinic acid, gallic acid, epicatechin, trimethylamine, N-acetylglucosamine, N-acetyltyrosine, β-sitosterol, 2,3-butanediol, lactic acid, caffeic acid, phydroxybenzoic acid, α-tocopherol, β-cryptoxanthin and kaempferol. The non-infected leaf showed higher level of sucrose, xylose, α-glucose, S-sulfocysteine and indole-3-acetic acid. NMR-based metabolomics applied in this study reveals that G. boninense alters a manifold of primary and secondary compounds in oil palm leaf. Southern Cross Publishing 2020-06-19 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/86908/1/NMR-based%20metabolomics%20reveals.pdf Isha, Azizul and Yusof, Nor Azah and Abdullah, Siti Nor Akmar and Wong, Mui Yun and Osman, Rosiah (2020) NMR-based metabolomics reveals effect of Ganoderma boninense infection on oil palm leaf at 30 days post-infection. Plant OMICS, 13 (1). 15 - 20. ISSN 1836-0661; ESSN: 1836-3644 https://www.pomics.com/may2020.html 10.21475/POJ.13.01.20.p2071 |
| spellingShingle | Isha, Azizul Yusof, Nor Azah Abdullah, Siti Nor Akmar Wong, Mui Yun Osman, Rosiah NMR-based metabolomics reveals effect of Ganoderma boninense infection on oil palm leaf at 30 days post-infection |
| title | NMR-based metabolomics reveals effect of Ganoderma boninense infection on oil palm leaf at 30 days post-infection |
| title_full | NMR-based metabolomics reveals effect of Ganoderma boninense infection on oil palm leaf at 30 days post-infection |
| title_fullStr | NMR-based metabolomics reveals effect of Ganoderma boninense infection on oil palm leaf at 30 days post-infection |
| title_full_unstemmed | NMR-based metabolomics reveals effect of Ganoderma boninense infection on oil palm leaf at 30 days post-infection |
| title_short | NMR-based metabolomics reveals effect of Ganoderma boninense infection on oil palm leaf at 30 days post-infection |
| title_sort | nmr-based metabolomics reveals effect of ganoderma boninense infection on oil palm leaf at 30 days post-infection |
| url | http://psasir.upm.edu.my/id/eprint/86908/ http://psasir.upm.edu.my/id/eprint/86908/ http://psasir.upm.edu.my/id/eprint/86908/ http://psasir.upm.edu.my/id/eprint/86908/1/NMR-based%20metabolomics%20reveals.pdf |