Micropropagation and essential oil characterization of Plectranthus amboinicus (Lour.) Sprengel, an aromatic medicinal plant
Plectranthus amboinicus (Loureiro) Sprengel is a therapeutically valued herb highly sought by various industries. This has led to its uncontrolled harvest from the wild leading to the risk of extinction. As an alternative, commercial propagation of P. amboinicus is desirable to avoid further depleti...
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| Format: | Article |
| Language: | English |
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Springer Verlag
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/86756/ http://psasir.upm.edu.my/id/eprint/86756/1/Micropropagation%20and%20essential%20oil%20characterization.pdf |
| _version_ | 1848860280096817152 |
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| author | Arumugam, Greetha Sinniah, Uma Rani Swamy, Mallappa Kumara Lynch, Paul T. |
| author_facet | Arumugam, Greetha Sinniah, Uma Rani Swamy, Mallappa Kumara Lynch, Paul T. |
| author_sort | Arumugam, Greetha |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Plectranthus amboinicus (Loureiro) Sprengel is a therapeutically valued herb highly sought by various industries. This has led to its uncontrolled harvest from the wild leading to the risk of extinction. As an alternative, commercial propagation of P. amboinicus is desirable to avoid further depletion of wild populations. This paper describes the establishment of an efficient protocol for the multiplication, rooting and acclimatization of P. amboinicus. The greatest frequency of shoot multiplication of P. amboinicus was on semi-solid Murashige and Skoog (MS) medium supplemented with 0.4 mg L−1 6-benzylaminopurine (BAP). Media supplemented with higher concentrations of BAP or used in combination with gibberellic acid (GA3) produced abnormal plantlets. Optimal rooting was observed on plantlets cultured in half strength MS medium. Acclimatization was achieved by transferring the rooted plants onto sterile peat-moss moistened with MS medium and exposed to the glasshouse environment gradually over a period of 2 months. P. amboinicus responded readily to tissue culture, but careful attention was required to media formulation to avoid abnormal plantlet development. The composition of essential oils extracted from field grown plants and in vitro microshoots were comparable. |
| first_indexed | 2025-11-15T12:42:43Z |
| format | Article |
| id | upm-86756 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:42:43Z |
| publishDate | 2020 |
| publisher | Springer Verlag |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-867562021-11-16T03:08:16Z http://psasir.upm.edu.my/id/eprint/86756/ Micropropagation and essential oil characterization of Plectranthus amboinicus (Lour.) Sprengel, an aromatic medicinal plant Arumugam, Greetha Sinniah, Uma Rani Swamy, Mallappa Kumara Lynch, Paul T. Plectranthus amboinicus (Loureiro) Sprengel is a therapeutically valued herb highly sought by various industries. This has led to its uncontrolled harvest from the wild leading to the risk of extinction. As an alternative, commercial propagation of P. amboinicus is desirable to avoid further depletion of wild populations. This paper describes the establishment of an efficient protocol for the multiplication, rooting and acclimatization of P. amboinicus. The greatest frequency of shoot multiplication of P. amboinicus was on semi-solid Murashige and Skoog (MS) medium supplemented with 0.4 mg L−1 6-benzylaminopurine (BAP). Media supplemented with higher concentrations of BAP or used in combination with gibberellic acid (GA3) produced abnormal plantlets. Optimal rooting was observed on plantlets cultured in half strength MS medium. Acclimatization was achieved by transferring the rooted plants onto sterile peat-moss moistened with MS medium and exposed to the glasshouse environment gradually over a period of 2 months. P. amboinicus responded readily to tissue culture, but careful attention was required to media formulation to avoid abnormal plantlet development. The composition of essential oils extracted from field grown plants and in vitro microshoots were comparable. Springer Verlag 2020-02-24 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/86756/1/Micropropagation%20and%20essential%20oil%20characterization.pdf Arumugam, Greetha and Sinniah, Uma Rani and Swamy, Mallappa Kumara and Lynch, Paul T. (2020) Micropropagation and essential oil characterization of Plectranthus amboinicus (Lour.) Sprengel, an aromatic medicinal plant. In Vitro Cellular & Developmental Biology - Plant, 56 (4). 491 - 503. ISSN 1054-5476; ESSN: 1475-2689 https://link.springer.com/article/10.1007/s11627-020-10056-1 10.1007/s11627-020-10056-1 |
| spellingShingle | Arumugam, Greetha Sinniah, Uma Rani Swamy, Mallappa Kumara Lynch, Paul T. Micropropagation and essential oil characterization of Plectranthus amboinicus (Lour.) Sprengel, an aromatic medicinal plant |
| title | Micropropagation and essential oil characterization of Plectranthus amboinicus (Lour.) Sprengel, an aromatic medicinal plant |
| title_full | Micropropagation and essential oil characterization of Plectranthus amboinicus (Lour.) Sprengel, an aromatic medicinal plant |
| title_fullStr | Micropropagation and essential oil characterization of Plectranthus amboinicus (Lour.) Sprengel, an aromatic medicinal plant |
| title_full_unstemmed | Micropropagation and essential oil characterization of Plectranthus amboinicus (Lour.) Sprengel, an aromatic medicinal plant |
| title_short | Micropropagation and essential oil characterization of Plectranthus amboinicus (Lour.) Sprengel, an aromatic medicinal plant |
| title_sort | micropropagation and essential oil characterization of plectranthus amboinicus (lour.) sprengel, an aromatic medicinal plant |
| url | http://psasir.upm.edu.my/id/eprint/86756/ http://psasir.upm.edu.my/id/eprint/86756/ http://psasir.upm.edu.my/id/eprint/86756/ http://psasir.upm.edu.my/id/eprint/86756/1/Micropropagation%20and%20essential%20oil%20characterization.pdf |