Main structural targets for engineering lipase substrate specificity
Microbial lipases represent one of the most important groups of biotechnological biocatalysts. However, the high-level production of lipases requires an understanding of the molecular mechanisms of gene expression, folding, and secretion processes. Stable, selective, and productive lipase is essenti...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Multidisciplinary Digital Publishing Institute
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/86618/ http://psasir.upm.edu.my/id/eprint/86618/1/Main%20structural%20targets%20.pdf |
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| author | Albayati, Samah Hashim Masomian, Malihe Ishak, Siti Nor Hasmah Mohamad Ali, Mohd Shukuri Leow, Adam Thean Chor Mohd Shariff, Fairolniza Muhd Noor, Noor Dina Raja Abdul Rahman, Raja Noor Zaliha |
| author_facet | Albayati, Samah Hashim Masomian, Malihe Ishak, Siti Nor Hasmah Mohamad Ali, Mohd Shukuri Leow, Adam Thean Chor Mohd Shariff, Fairolniza Muhd Noor, Noor Dina Raja Abdul Rahman, Raja Noor Zaliha |
| author_sort | Albayati, Samah Hashim |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Microbial lipases represent one of the most important groups of biotechnological biocatalysts. However, the high-level production of lipases requires an understanding of the molecular mechanisms of gene expression, folding, and secretion processes. Stable, selective, and productive lipase is essential for modern chemical industries, as most lipases cannot work in different process conditions. However, the screening and isolation of a new lipase with desired and specific properties would be time consuming, and costly, so researchers typically modify an available lipase with a certain potential for minimizing cost. Improving enzyme properties is associated with altering the enzymatic structure by changing one or several amino acids in the protein sequence. This review detailed the main sources, classification, structural properties, and mutagenic approaches, such as rational design (site direct mutagenesis, iterative saturation mutagenesis) and direct evolution (error prone PCR, DNA shuffling), for achieving modification goals. Here, both techniques were reviewed, with different results for lipase engineering, with a particular focus on improving or changing lipase specificity. Changing the amino acid sequences of the binding pocket or lid region of the lipase led to remarkable enzyme substrate specificity and enantioselectivity improvement. Site-directed mutagenesis is one of the appropriate methods to alter the enzyme sequence, as compared to random mutagenesis, such as error-prone PCR. This contribution has summarized and evaluated several experimental studies on modifying the substrate specificity of lipases. |
| first_indexed | 2025-11-15T12:42:11Z |
| format | Article |
| id | upm-86618 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:42:11Z |
| publishDate | 2020 |
| publisher | Multidisciplinary Digital Publishing Institute |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-866182021-10-13T05:23:04Z http://psasir.upm.edu.my/id/eprint/86618/ Main structural targets for engineering lipase substrate specificity Albayati, Samah Hashim Masomian, Malihe Ishak, Siti Nor Hasmah Mohamad Ali, Mohd Shukuri Leow, Adam Thean Chor Mohd Shariff, Fairolniza Muhd Noor, Noor Dina Raja Abdul Rahman, Raja Noor Zaliha Microbial lipases represent one of the most important groups of biotechnological biocatalysts. However, the high-level production of lipases requires an understanding of the molecular mechanisms of gene expression, folding, and secretion processes. Stable, selective, and productive lipase is essential for modern chemical industries, as most lipases cannot work in different process conditions. However, the screening and isolation of a new lipase with desired and specific properties would be time consuming, and costly, so researchers typically modify an available lipase with a certain potential for minimizing cost. Improving enzyme properties is associated with altering the enzymatic structure by changing one or several amino acids in the protein sequence. This review detailed the main sources, classification, structural properties, and mutagenic approaches, such as rational design (site direct mutagenesis, iterative saturation mutagenesis) and direct evolution (error prone PCR, DNA shuffling), for achieving modification goals. Here, both techniques were reviewed, with different results for lipase engineering, with a particular focus on improving or changing lipase specificity. Changing the amino acid sequences of the binding pocket or lid region of the lipase led to remarkable enzyme substrate specificity and enantioselectivity improvement. Site-directed mutagenesis is one of the appropriate methods to alter the enzyme sequence, as compared to random mutagenesis, such as error-prone PCR. This contribution has summarized and evaluated several experimental studies on modifying the substrate specificity of lipases. Multidisciplinary Digital Publishing Institute 2020-07 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/86618/1/Main%20structural%20targets%20.pdf Albayati, Samah Hashim and Masomian, Malihe and Ishak, Siti Nor Hasmah and Mohamad Ali, Mohd Shukuri and Leow, Adam Thean Chor and Mohd Shariff, Fairolniza and Muhd Noor, Noor Dina and Raja Abdul Rahman, Raja Noor Zaliha (2020) Main structural targets for engineering lipase substrate specificity. Catalysts, 10 (7). pp. 1-43. ISSN 2073-4344 https://www.mdpi.com/2073-4344/10/7/747 10.3390/catal10070747 |
| spellingShingle | Albayati, Samah Hashim Masomian, Malihe Ishak, Siti Nor Hasmah Mohamad Ali, Mohd Shukuri Leow, Adam Thean Chor Mohd Shariff, Fairolniza Muhd Noor, Noor Dina Raja Abdul Rahman, Raja Noor Zaliha Main structural targets for engineering lipase substrate specificity |
| title | Main structural targets for engineering lipase substrate specificity |
| title_full | Main structural targets for engineering lipase substrate specificity |
| title_fullStr | Main structural targets for engineering lipase substrate specificity |
| title_full_unstemmed | Main structural targets for engineering lipase substrate specificity |
| title_short | Main structural targets for engineering lipase substrate specificity |
| title_sort | main structural targets for engineering lipase substrate specificity |
| url | http://psasir.upm.edu.my/id/eprint/86618/ http://psasir.upm.edu.my/id/eprint/86618/ http://psasir.upm.edu.my/id/eprint/86618/ http://psasir.upm.edu.my/id/eprint/86618/1/Main%20structural%20targets%20.pdf |