The Development of Photosystem II-Herbicide Detection Method Using Microalgae as a Biosensor
Herbicides have been found to be significant pollutants of water bodies. In order to control and monitor the pollution, a convenient, sensitive and reliable method is needed. The main objective of this study is to develop a new method to measure the fluorescence yield of microalgae in order to d...
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| Format: | Thesis |
| Language: | English English |
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1997
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| Online Access: | http://psasir.upm.edu.my/id/eprint/8634/ http://psasir.upm.edu.my/id/eprint/8634/1/FSAS_1997_19_A.pdf |
| _version_ | 1848840924332818432 |
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| author | Desa, Shakinaz |
| author_facet | Desa, Shakinaz |
| author_sort | Desa, Shakinaz |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Herbicides have been found to be significant pollutants of water
bodies. In order to control and monitor the pollution, a convenient,
sensitive and reliable method is needed. The main objective of this
study is to develop a new method to measure the fluorescence yield of
microalgae in order to detect photosystem II (PSII) specific herbicides
in polluted water. The system was based on a simple mixing of
microalgae with PSII specific herbicides. The measured signal was in the form of fluorescence intensity. The study involved the screening of
test organisms, determining the best condition of the culture and
obtaining a standard curve for each of the PSII herbicides. Only three
herbicides were studied; atrazine, simazine and diuron. Chlorella
vulgaris was found as the most suitable test organism compared with
Chlorococcum sp., Scenedesmus quadriqauda and Selenastrum sp ..
Nutrient replacement culturing method was selected as the best
condition to grow C. vulgaris. A standard curve was determined for
each of the herbicides. C. vulgaris was able to signal atrazine's
concentration above 0.04µM according to the standard curve equation.
Simazine can be signalled at above 0.05µM from its equation, while
diuron can be signalled at above than O.09µM. However, -this basic
study needs more infonnation and improvement to be done in the
future, so that its full potential can be realised. |
| first_indexed | 2025-11-15T07:35:04Z |
| format | Thesis |
| id | upm-8634 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T07:35:04Z |
| publishDate | 1997 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-86342012-05-08T09:20:00Z http://psasir.upm.edu.my/id/eprint/8634/ The Development of Photosystem II-Herbicide Detection Method Using Microalgae as a Biosensor Desa, Shakinaz Herbicides have been found to be significant pollutants of water bodies. In order to control and monitor the pollution, a convenient, sensitive and reliable method is needed. The main objective of this study is to develop a new method to measure the fluorescence yield of microalgae in order to detect photosystem II (PSII) specific herbicides in polluted water. The system was based on a simple mixing of microalgae with PSII specific herbicides. The measured signal was in the form of fluorescence intensity. The study involved the screening of test organisms, determining the best condition of the culture and obtaining a standard curve for each of the PSII herbicides. Only three herbicides were studied; atrazine, simazine and diuron. Chlorella vulgaris was found as the most suitable test organism compared with Chlorococcum sp., Scenedesmus quadriqauda and Selenastrum sp .. Nutrient replacement culturing method was selected as the best condition to grow C. vulgaris. A standard curve was determined for each of the herbicides. C. vulgaris was able to signal atrazine's concentration above 0.04µM according to the standard curve equation. Simazine can be signalled at above 0.05µM from its equation, while diuron can be signalled at above than O.09µM. However, -this basic study needs more infonnation and improvement to be done in the future, so that its full potential can be realised. 1997 Thesis NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/8634/1/FSAS_1997_19_A.pdf Desa, Shakinaz (1997) The Development of Photosystem II-Herbicide Detection Method Using Microalgae as a Biosensor. Masters thesis, Universiti Putra Malaysia. Microalgae Herbicides - Physiological effect English |
| spellingShingle | Microalgae Herbicides - Physiological effect Desa, Shakinaz The Development of Photosystem II-Herbicide Detection Method Using Microalgae as a Biosensor |
| title | The Development of Photosystem II-Herbicide Detection Method Using Microalgae as a Biosensor
|
| title_full | The Development of Photosystem II-Herbicide Detection Method Using Microalgae as a Biosensor
|
| title_fullStr | The Development of Photosystem II-Herbicide Detection Method Using Microalgae as a Biosensor
|
| title_full_unstemmed | The Development of Photosystem II-Herbicide Detection Method Using Microalgae as a Biosensor
|
| title_short | The Development of Photosystem II-Herbicide Detection Method Using Microalgae as a Biosensor
|
| title_sort | development of photosystem ii-herbicide detection method using microalgae as a biosensor |
| topic | Microalgae Herbicides - Physiological effect |
| url | http://psasir.upm.edu.my/id/eprint/8634/ http://psasir.upm.edu.my/id/eprint/8634/1/FSAS_1997_19_A.pdf |