Effects of marine microalgae and cyanobacteria towards the morphology and survival rate of zebrafish larvae, Danio rerio

The zebrafish (Danio rerio) is becoming internationally recognized as a test model organsims representing animals as well as human. An experiment was conducted to study selected different microalgae and cyanobacteria extracts (Spirulina platensis, Monoraphidium sp., Halamphora sp., Chaetoceros sp. a...

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Main Author: Toh, En Chun
Format: Project Paper Report
Language:English
Published: 2018
Online Access:http://psasir.upm.edu.my/id/eprint/85782/
http://psasir.upm.edu.my/id/eprint/85782/1/FP%202018%2022%20IR.pdf
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author Toh, En Chun
author_facet Toh, En Chun
author_sort Toh, En Chun
building UPM Institutional Repository
collection Online Access
description The zebrafish (Danio rerio) is becoming internationally recognized as a test model organsims representing animals as well as human. An experiment was conducted to study selected different microalgae and cyanobacteria extracts (Spirulina platensis, Monoraphidium sp., Halamphora sp., Chaetoceros sp. and Chattonella marina) on the D. rerio larvae morphology and survival rate. Samples of microalgae and cyanobacteria at stationary phase were extracted using 95% methanol and were exposed to D. rerio eggs at the concentrations of 0 ppm, 10 ppm, 20 ppm, 50 ppm and 100 ppm. The embryonic development was observed daily until hatched. The results showed that no significant differences between egg morphology were detected through the exposure from 0 ppm until 100 ppm. The experiment was then repeated through an increase of concentration up to 200 ppm. It was shown that a toxic Chattonella marina induced negative impacts towards the zebrafish larvae morphology causing deformation with low survival rate at (46.67%) compared to control treatment (0ppm, sterile distilled water) which have 100% survival rate. This indicated that the Chattonella marina is lethal towards the zebrafish larvae at high concentration.
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institution Universiti Putra Malaysia
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publishDate 2018
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spelling upm-857822021-06-22T05:20:05Z http://psasir.upm.edu.my/id/eprint/85782/ Effects of marine microalgae and cyanobacteria towards the morphology and survival rate of zebrafish larvae, Danio rerio Toh, En Chun The zebrafish (Danio rerio) is becoming internationally recognized as a test model organsims representing animals as well as human. An experiment was conducted to study selected different microalgae and cyanobacteria extracts (Spirulina platensis, Monoraphidium sp., Halamphora sp., Chaetoceros sp. and Chattonella marina) on the D. rerio larvae morphology and survival rate. Samples of microalgae and cyanobacteria at stationary phase were extracted using 95% methanol and were exposed to D. rerio eggs at the concentrations of 0 ppm, 10 ppm, 20 ppm, 50 ppm and 100 ppm. The embryonic development was observed daily until hatched. The results showed that no significant differences between egg morphology were detected through the exposure from 0 ppm until 100 ppm. The experiment was then repeated through an increase of concentration up to 200 ppm. It was shown that a toxic Chattonella marina induced negative impacts towards the zebrafish larvae morphology causing deformation with low survival rate at (46.67%) compared to control treatment (0ppm, sterile distilled water) which have 100% survival rate. This indicated that the Chattonella marina is lethal towards the zebrafish larvae at high concentration. 2018 Project Paper Report NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/85782/1/FP%202018%2022%20IR.pdf Toh, En Chun (2018) Effects of marine microalgae and cyanobacteria towards the morphology and survival rate of zebrafish larvae, Danio rerio. [Project Paper Report]
spellingShingle Toh, En Chun
Effects of marine microalgae and cyanobacteria towards the morphology and survival rate of zebrafish larvae, Danio rerio
title Effects of marine microalgae and cyanobacteria towards the morphology and survival rate of zebrafish larvae, Danio rerio
title_full Effects of marine microalgae and cyanobacteria towards the morphology and survival rate of zebrafish larvae, Danio rerio
title_fullStr Effects of marine microalgae and cyanobacteria towards the morphology and survival rate of zebrafish larvae, Danio rerio
title_full_unstemmed Effects of marine microalgae and cyanobacteria towards the morphology and survival rate of zebrafish larvae, Danio rerio
title_short Effects of marine microalgae and cyanobacteria towards the morphology and survival rate of zebrafish larvae, Danio rerio
title_sort effects of marine microalgae and cyanobacteria towards the morphology and survival rate of zebrafish larvae, danio rerio
url http://psasir.upm.edu.my/id/eprint/85782/
http://psasir.upm.edu.my/id/eprint/85782/1/FP%202018%2022%20IR.pdf