A Transient Assay to Evaluate the Expression of Polyhydroxybutyrate Genes Regulated by Oil Palm Mesocarp-Specific Promoter
The promoter of the oil palm metallothionein-like gene (MT3-A) demonstrated mesocarp-specific activity in functional analysis using transient expression assay of reporter gene in bombarded oil palm tissue slices. In order to investigate the tissue-specific expression of polyhydroxybutyrate (PHB) bio...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Springer Verlag
2008
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| Online Access: | http://psasir.upm.edu.my/id/eprint/857/ |
| _version_ | 1848838871389831168 |
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| author | Omidvar, Vahid Abdullah, Siti Nor Akmar Mahmood, Maziah Abdul Aziz, Maheran |
| author_facet | Omidvar, Vahid Abdullah, Siti Nor Akmar Mahmood, Maziah Abdul Aziz, Maheran |
| author_sort | Omidvar, Vahid |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The promoter of the oil palm metallothionein-like gene (MT3-A) demonstrated mesocarp-specific activity in functional analysis using transient expression assay of reporter gene in bombarded oil palm tissue slices. In order to investigate the tissue-specific expression of polyhydroxybutyrate (PHB) biosynthetic pathway genes, a multi-gene construct carrying PHB genes fused to the oil palm MT3-A promoter was co-transferred with a construct carrying GFP reporter gene using microprojectile bombardment targeting the mesocarp and leaf tissues of the oil palm. Transcriptional analysis using RT-PCR revealed successful transcription of all the three phbA, phbB, and phbC genes in transiently transformed mesocarp but not in transiently transformed leaf tissues. Furthermore, all the three expected sizes of PHB-encoded protein products were only detected in transiently transformed mesocarp tissues on a silver stained polyacrylamide gel. Western blot analysis using polyclonal antibody specific for phbB product confirmed successful translation of phbB mRNA transcript into protein product. This study provided valuable information, supporting the future engineering of PHB-producing transgenic palms. |
| first_indexed | 2025-11-15T07:02:26Z |
| format | Article |
| id | upm-857 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T07:02:26Z |
| publishDate | 2008 |
| publisher | Springer Verlag |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-8572011-10-30T05:11:23Z http://psasir.upm.edu.my/id/eprint/857/ A Transient Assay to Evaluate the Expression of Polyhydroxybutyrate Genes Regulated by Oil Palm Mesocarp-Specific Promoter Omidvar, Vahid Abdullah, Siti Nor Akmar Mahmood, Maziah Abdul Aziz, Maheran The promoter of the oil palm metallothionein-like gene (MT3-A) demonstrated mesocarp-specific activity in functional analysis using transient expression assay of reporter gene in bombarded oil palm tissue slices. In order to investigate the tissue-specific expression of polyhydroxybutyrate (PHB) biosynthetic pathway genes, a multi-gene construct carrying PHB genes fused to the oil palm MT3-A promoter was co-transferred with a construct carrying GFP reporter gene using microprojectile bombardment targeting the mesocarp and leaf tissues of the oil palm. Transcriptional analysis using RT-PCR revealed successful transcription of all the three phbA, phbB, and phbC genes in transiently transformed mesocarp but not in transiently transformed leaf tissues. Furthermore, all the three expected sizes of PHB-encoded protein products were only detected in transiently transformed mesocarp tissues on a silver stained polyacrylamide gel. Western blot analysis using polyclonal antibody specific for phbB product confirmed successful translation of phbB mRNA transcript into protein product. This study provided valuable information, supporting the future engineering of PHB-producing transgenic palms. Springer Verlag 2008 Article PeerReviewed Omidvar, Vahid and Abdullah, Siti Nor Akmar and Mahmood, Maziah and Abdul Aziz, Maheran (2008) A Transient Assay to Evaluate the Expression of Polyhydroxybutyrate Genes Regulated by Oil Palm Mesocarp-Specific Promoter. Plant Cell Reports, 27 (9). pp. 1451-1459. ISSN 0721-7714 http://dx.doi.org/10.1007/s00299-008-0565-2 English |
| spellingShingle | Omidvar, Vahid Abdullah, Siti Nor Akmar Mahmood, Maziah Abdul Aziz, Maheran A Transient Assay to Evaluate the Expression of Polyhydroxybutyrate Genes Regulated by Oil Palm Mesocarp-Specific Promoter |
| title | A Transient Assay to Evaluate the Expression of Polyhydroxybutyrate Genes Regulated by Oil Palm Mesocarp-Specific Promoter |
| title_full | A Transient Assay to Evaluate the Expression of Polyhydroxybutyrate Genes Regulated by Oil Palm Mesocarp-Specific Promoter |
| title_fullStr | A Transient Assay to Evaluate the Expression of Polyhydroxybutyrate Genes Regulated by Oil Palm Mesocarp-Specific Promoter |
| title_full_unstemmed | A Transient Assay to Evaluate the Expression of Polyhydroxybutyrate Genes Regulated by Oil Palm Mesocarp-Specific Promoter |
| title_short | A Transient Assay to Evaluate the Expression of Polyhydroxybutyrate Genes Regulated by Oil Palm Mesocarp-Specific Promoter |
| title_sort | transient assay to evaluate the expression of polyhydroxybutyrate genes regulated by oil palm mesocarp-specific promoter |
| url | http://psasir.upm.edu.my/id/eprint/857/ http://psasir.upm.edu.my/id/eprint/857/ |