Lipid and blood pressure lowering potential of Mikania micrantha through enzymatic inhibition

Mikania micrantha Kunth (Asteraceae) is a plant that traditionally used to reduce the risk of hyperlipidemia and hypertension. This study was aimed to examine the in vitro potential of different parts of M. micrantha (leaves and stems) extracts to inhibit enzymes relevant to hyperlipidemia i.e., pan...

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Main Authors: Shafie, Nurul Husna, Ishak, Amirah Haziyah, Mohd Esa, Norhaizan, Bahari, Hasnah
Format: Conference or Workshop Item
Language:English
Published: MDPI 2020
Online Access:http://psasir.upm.edu.my/id/eprint/85431/
http://psasir.upm.edu.my/id/eprint/85431/1/85431.pdf
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author Shafie, Nurul Husna
Ishak, Amirah Haziyah
Mohd Esa, Norhaizan
Bahari, Hasnah
author_facet Shafie, Nurul Husna
Ishak, Amirah Haziyah
Mohd Esa, Norhaizan
Bahari, Hasnah
author_sort Shafie, Nurul Husna
building UPM Institutional Repository
collection Online Access
description Mikania micrantha Kunth (Asteraceae) is a plant that traditionally used to reduce the risk of hyperlipidemia and hypertension. This study was aimed to examine the in vitro potential of different parts of M. micrantha (leaves and stems) extracts to inhibit enzymes relevant to hyperlipidemia i.e., pancreatic lipase (PL), lipoprotein lipase (LPL) and 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR), and also hypertension i.e., angiotensin-I converting enzyme (ACE). The inhibitory activities of hot water, cold water, 70% ethanol and ethyl acetate extracts of the leaves and stems of M. micrantha against the enzymes were determined spectrophotometrically using PL, LPL, HMGR, and ACE inhibition assays. The ethanol stems (ETS) extract exhibited the highest PL inhibitory activity (IC50=4.49±2.50 μg/mL) and showed no significant (p > 0.05) difference with orlistat (IC50=0.31±0.01 μg/mL). The ethanol leaves (ETL) extract showed the highest LPL (IC50=1.42±0.48 μg/mL) and HMGR inhibitory activities (50.12±3.44%). Hot water stems (HWS) showed the greatest ACE inhibition (97.47±1.19%), but not significant (p > 0.05) when compared to other extracts and captopril (98.42±0.93%). Overall, all extracts exhibited remarkable inhibitory activity against PL, LPL, HMGR, and ACE. This study demonstrated the potential of M. micrantha extracts as anti-hyperlipidemic and anti-hypertensive agents.
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format Conference or Workshop Item
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institution Universiti Putra Malaysia
institution_category Local University
language English
last_indexed 2025-11-15T12:39:37Z
publishDate 2020
publisher MDPI
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spelling upm-854312025-06-03T02:24:05Z http://psasir.upm.edu.my/id/eprint/85431/ Lipid and blood pressure lowering potential of Mikania micrantha through enzymatic inhibition Shafie, Nurul Husna Ishak, Amirah Haziyah Mohd Esa, Norhaizan Bahari, Hasnah Mikania micrantha Kunth (Asteraceae) is a plant that traditionally used to reduce the risk of hyperlipidemia and hypertension. This study was aimed to examine the in vitro potential of different parts of M. micrantha (leaves and stems) extracts to inhibit enzymes relevant to hyperlipidemia i.e., pancreatic lipase (PL), lipoprotein lipase (LPL) and 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR), and also hypertension i.e., angiotensin-I converting enzyme (ACE). The inhibitory activities of hot water, cold water, 70% ethanol and ethyl acetate extracts of the leaves and stems of M. micrantha against the enzymes were determined spectrophotometrically using PL, LPL, HMGR, and ACE inhibition assays. The ethanol stems (ETS) extract exhibited the highest PL inhibitory activity (IC50=4.49±2.50 μg/mL) and showed no significant (p > 0.05) difference with orlistat (IC50=0.31±0.01 μg/mL). The ethanol leaves (ETL) extract showed the highest LPL (IC50=1.42±0.48 μg/mL) and HMGR inhibitory activities (50.12±3.44%). Hot water stems (HWS) showed the greatest ACE inhibition (97.47±1.19%), but not significant (p > 0.05) when compared to other extracts and captopril (98.42±0.93%). Overall, all extracts exhibited remarkable inhibitory activity against PL, LPL, HMGR, and ACE. This study demonstrated the potential of M. micrantha extracts as anti-hyperlipidemic and anti-hypertensive agents. MDPI 2020-11-03 Conference or Workshop Item PeerReviewed text en http://psasir.upm.edu.my/id/eprint/85431/1/85431.pdf Shafie, Nurul Husna and Ishak, Amirah Haziyah and Mohd Esa, Norhaizan and Bahari, Hasnah (2020) Lipid and blood pressure lowering potential of Mikania micrantha through enzymatic inhibition. In: The 1st International Electronic Conference on Nutrients, 2–15 November 2020, Online. . http://sciforum.net/conference/IECN2020/paper/7172 10.3390/IECN2020-07172
spellingShingle Shafie, Nurul Husna
Ishak, Amirah Haziyah
Mohd Esa, Norhaizan
Bahari, Hasnah
Lipid and blood pressure lowering potential of Mikania micrantha through enzymatic inhibition
title Lipid and blood pressure lowering potential of Mikania micrantha through enzymatic inhibition
title_full Lipid and blood pressure lowering potential of Mikania micrantha through enzymatic inhibition
title_fullStr Lipid and blood pressure lowering potential of Mikania micrantha through enzymatic inhibition
title_full_unstemmed Lipid and blood pressure lowering potential of Mikania micrantha through enzymatic inhibition
title_short Lipid and blood pressure lowering potential of Mikania micrantha through enzymatic inhibition
title_sort lipid and blood pressure lowering potential of mikania micrantha through enzymatic inhibition
url http://psasir.upm.edu.my/id/eprint/85431/
http://psasir.upm.edu.my/id/eprint/85431/
http://psasir.upm.edu.my/id/eprint/85431/
http://psasir.upm.edu.my/id/eprint/85431/1/85431.pdf