Isolation and characterization of Streptococcus from dental plaque samples

Dental caries is still a prevalent chronic condition that affecting health in the worldwide population. Other oral diseases, such as infection and tooth loss, are also very common. These diseases normally associated with discomfort and pain, eating and speech difficulties. Periodontal disease is...

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Main Author: Chan, Weng Yee
Format: Project Paper Report
Language:English
Published: 2015
Online Access:http://psasir.upm.edu.my/id/eprint/85108/
http://psasir.upm.edu.my/id/eprint/85108/1/FBSB%202015%2097%20-%20IR.pdf
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author Chan, Weng Yee
author_facet Chan, Weng Yee
author_sort Chan, Weng Yee
building UPM Institutional Repository
collection Online Access
description Dental caries is still a prevalent chronic condition that affecting health in the worldwide population. Other oral diseases, such as infection and tooth loss, are also very common. These diseases normally associated with discomfort and pain, eating and speech difficulties. Periodontal disease is an inflammatory infection on the gums, connective tissues and jaw bone that support the teeth. The root of these complications is formation of dental plaque by specific group of bacteria. Prevention practices, such as brushing teeth twice a day, using dental floss and antibacterial rinse, eating a balanced diet are not practiced. Therefore, the most efficient method to overcome this issue is eliminating the causative agents. This study is to isolate and characterize dental plaque causing bacteria. The bacteria were isolated from dental plaque sample. Preliminary identifications performed were macroscopic, microscopic morphology and biochemical tests. The bacteria isolated were characterized using Restriction Fragment Length Polymorphism (RFLP) and DNA sequencing. Three isolates were selected and identified as Streptococcus mitis and Streptococcus oralis.
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spelling upm-851082021-12-27T04:07:00Z http://psasir.upm.edu.my/id/eprint/85108/ Isolation and characterization of Streptococcus from dental plaque samples Chan, Weng Yee Dental caries is still a prevalent chronic condition that affecting health in the worldwide population. Other oral diseases, such as infection and tooth loss, are also very common. These diseases normally associated with discomfort and pain, eating and speech difficulties. Periodontal disease is an inflammatory infection on the gums, connective tissues and jaw bone that support the teeth. The root of these complications is formation of dental plaque by specific group of bacteria. Prevention practices, such as brushing teeth twice a day, using dental floss and antibacterial rinse, eating a balanced diet are not practiced. Therefore, the most efficient method to overcome this issue is eliminating the causative agents. This study is to isolate and characterize dental plaque causing bacteria. The bacteria were isolated from dental plaque sample. Preliminary identifications performed were macroscopic, microscopic morphology and biochemical tests. The bacteria isolated were characterized using Restriction Fragment Length Polymorphism (RFLP) and DNA sequencing. Three isolates were selected and identified as Streptococcus mitis and Streptococcus oralis. 2015-06 Project Paper Report NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/85108/1/FBSB%202015%2097%20-%20IR.pdf Chan, Weng Yee (2015) Isolation and characterization of Streptococcus from dental plaque samples. [Project Paper Report]
spellingShingle Chan, Weng Yee
Isolation and characterization of Streptococcus from dental plaque samples
title Isolation and characterization of Streptococcus from dental plaque samples
title_full Isolation and characterization of Streptococcus from dental plaque samples
title_fullStr Isolation and characterization of Streptococcus from dental plaque samples
title_full_unstemmed Isolation and characterization of Streptococcus from dental plaque samples
title_short Isolation and characterization of Streptococcus from dental plaque samples
title_sort isolation and characterization of streptococcus from dental plaque samples
url http://psasir.upm.edu.my/id/eprint/85108/
http://psasir.upm.edu.my/id/eprint/85108/1/FBSB%202015%2097%20-%20IR.pdf