Immobilization of uricase onto graphene oxide
Immobilization of uricase onto graphene oxide was done by using two different methods, physical adsorption and also chemical modification of the surface of graphene oxide by using N’-(3-(dimethylamino)propyl)carbodiimide (EDC) and N-hydroxysuccinimide (NHS) as cross-linkers. Graphene oxide is proved...
| Main Author: | |
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| Format: | Project Paper Report |
| Language: | English |
| Published: |
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/85055/ http://psasir.upm.edu.my/id/eprint/85055/1/FBSB%202015%20121%20-%20IR.pdf |
| _version_ | 1848859980415893504 |
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| author | Sidek, Nur Amirah |
| author_facet | Sidek, Nur Amirah |
| author_sort | Sidek, Nur Amirah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Immobilization of uricase onto graphene oxide was done by using two different methods, physical adsorption and also chemical modification of the surface of graphene oxide by using N’-(3-(dimethylamino)propyl)carbodiimide (EDC) and N-hydroxysuccinimide (NHS) as cross-linkers. Graphene oxide is proved to be an excellent carrier for uricase immobilization due to it having a large surface area and abundant functional groups. The enzyme immobilization on graphene oxide can be done readily without using any cross-linking reagents or other additional surface modification. It can also be done by modifying the surface of graphene oxide for an enhanced performance. In this study, apart from using different methods, other paramaters such as the concentration of graphene oxide and also enzyme unit used are also varied to determine the best conditions for an optimum performance of the immobilized enzyme. The results obtained indicate that the immobilized enzyme perform better by using the chemical modification method, and the best parameters of uricase immobilization by this method were found as 1 mg/mL graphene oxide concentration and 1 u/ml uricase. |
| first_indexed | 2025-11-15T12:37:57Z |
| format | Project Paper Report |
| id | upm-85055 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:37:57Z |
| publishDate | 2015 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-850552021-07-13T04:45:12Z http://psasir.upm.edu.my/id/eprint/85055/ Immobilization of uricase onto graphene oxide Sidek, Nur Amirah Immobilization of uricase onto graphene oxide was done by using two different methods, physical adsorption and also chemical modification of the surface of graphene oxide by using N’-(3-(dimethylamino)propyl)carbodiimide (EDC) and N-hydroxysuccinimide (NHS) as cross-linkers. Graphene oxide is proved to be an excellent carrier for uricase immobilization due to it having a large surface area and abundant functional groups. The enzyme immobilization on graphene oxide can be done readily without using any cross-linking reagents or other additional surface modification. It can also be done by modifying the surface of graphene oxide for an enhanced performance. In this study, apart from using different methods, other paramaters such as the concentration of graphene oxide and also enzyme unit used are also varied to determine the best conditions for an optimum performance of the immobilized enzyme. The results obtained indicate that the immobilized enzyme perform better by using the chemical modification method, and the best parameters of uricase immobilization by this method were found as 1 mg/mL graphene oxide concentration and 1 u/ml uricase. 2015-06 Project Paper Report NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/85055/1/FBSB%202015%20121%20-%20IR.pdf Sidek, Nur Amirah (2015) Immobilization of uricase onto graphene oxide. [Project Paper Report] |
| spellingShingle | Sidek, Nur Amirah Immobilization of uricase onto graphene oxide |
| title | Immobilization of uricase onto graphene oxide |
| title_full | Immobilization of uricase onto graphene oxide |
| title_fullStr | Immobilization of uricase onto graphene oxide |
| title_full_unstemmed | Immobilization of uricase onto graphene oxide |
| title_short | Immobilization of uricase onto graphene oxide |
| title_sort | immobilization of uricase onto graphene oxide |
| url | http://psasir.upm.edu.my/id/eprint/85055/ http://psasir.upm.edu.my/id/eprint/85055/1/FBSB%202015%20121%20-%20IR.pdf |