Inhibitory effects of the crude extract from Phomopsis sp. on several pathogenic fungi

Endophytic fungi inhabiting plant hosts may contain fungitoxic properties and can be useful for biological control agents. An isolate of Phomopsis sp. from the mangrove tree has been determined if it has possessed antifungal properties. In this study, Phomopsis sp. was cultured in Potato Dextrose Br...

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Main Author: Rasidan, Rasyiffa' Hayati
Format: Project Paper Report
Language:English
Published: 2019
Online Access:http://psasir.upm.edu.my/id/eprint/84786/
http://psasir.upm.edu.my/id/eprint/84786/1/FH%202019%2043%20IR.pdf
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author Rasidan, Rasyiffa' Hayati
author_facet Rasidan, Rasyiffa' Hayati
author_sort Rasidan, Rasyiffa' Hayati
building UPM Institutional Repository
collection Online Access
description Endophytic fungi inhabiting plant hosts may contain fungitoxic properties and can be useful for biological control agents. An isolate of Phomopsis sp. from the mangrove tree has been determined if it has possessed antifungal properties. In this study, Phomopsis sp. was cultured in Potato Dextrose Broth (PDB) for 14 day. After which the mycelia were removed, the filtered culture was subjected to liquid-liquid extraction using ethyl acetate (EtOAc) as the solvent. Then, it was purified through a chromatography column using various solvents. Two eluents, hexane (PH) and the mixture of hexane + methanol (PMH), when tested against the pathogenic fungus (Ceratocystis fimbriata) gave 25% inhibitory effect. These findings indicate extraction of secondary metabolite from Phomopsis sp. have the antifungal ability as it can be used as biological control agent.
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format Project Paper Report
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institution Universiti Putra Malaysia
institution_category Local University
language English
last_indexed 2025-11-15T12:36:48Z
publishDate 2019
recordtype eprints
repository_type Digital Repository
spelling upm-847862021-03-25T00:39:06Z http://psasir.upm.edu.my/id/eprint/84786/ Inhibitory effects of the crude extract from Phomopsis sp. on several pathogenic fungi Rasidan, Rasyiffa' Hayati Endophytic fungi inhabiting plant hosts may contain fungitoxic properties and can be useful for biological control agents. An isolate of Phomopsis sp. from the mangrove tree has been determined if it has possessed antifungal properties. In this study, Phomopsis sp. was cultured in Potato Dextrose Broth (PDB) for 14 day. After which the mycelia were removed, the filtered culture was subjected to liquid-liquid extraction using ethyl acetate (EtOAc) as the solvent. Then, it was purified through a chromatography column using various solvents. Two eluents, hexane (PH) and the mixture of hexane + methanol (PMH), when tested against the pathogenic fungus (Ceratocystis fimbriata) gave 25% inhibitory effect. These findings indicate extraction of secondary metabolite from Phomopsis sp. have the antifungal ability as it can be used as biological control agent. 2019 Project Paper Report NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/84786/1/FH%202019%2043%20IR.pdf Rasidan, Rasyiffa' Hayati (2019) Inhibitory effects of the crude extract from Phomopsis sp. on several pathogenic fungi. [Project Paper Report]
spellingShingle Rasidan, Rasyiffa' Hayati
Inhibitory effects of the crude extract from Phomopsis sp. on several pathogenic fungi
title Inhibitory effects of the crude extract from Phomopsis sp. on several pathogenic fungi
title_full Inhibitory effects of the crude extract from Phomopsis sp. on several pathogenic fungi
title_fullStr Inhibitory effects of the crude extract from Phomopsis sp. on several pathogenic fungi
title_full_unstemmed Inhibitory effects of the crude extract from Phomopsis sp. on several pathogenic fungi
title_short Inhibitory effects of the crude extract from Phomopsis sp. on several pathogenic fungi
title_sort inhibitory effects of the crude extract from phomopsis sp. on several pathogenic fungi
url http://psasir.upm.edu.my/id/eprint/84786/
http://psasir.upm.edu.my/id/eprint/84786/1/FH%202019%2043%20IR.pdf