Propagation of Sealing Wax Palm, Cyrtostachys Renda Blume, Using in Vitro Techniques
A series of experiments on the culture of vegetative tissues and embryos of Cyrtostachys renda Blume were conducted to explore the possibility of in vitro culture as an alternative system of propagation. Isozyme typing of the vegetative tissues used as explants was also carried out. Unemerged l...
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| Format: | Thesis |
| Language: | English English |
| Published: |
1997
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| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/8381/ http://psasir.upm.edu.my/id/eprint/8381/1/FSMB_1997_10_A.pdf |
| Summary: | A series of experiments on the culture of vegetative
tissues and embryos of Cyrtostachys renda Blume were
conducted to explore the possibility of in vitro culture as
an alternative system of propagation. Isozyme typing of the
vegetative tissues used as explants was also carried out.
Unemerged leaf tissue of about 1cm with petiole
attached and probably including shoot (L1), was used as
explant. The preceding two 1-cm leaf sections before Ll
were also used as exalants. However,the youngest tissue
(L1) used was least-proned to browning and survived in
culture where as the preceding two sections died in culture.
Three different culture media were tested,namely
modified Murashige and Skoog (MMS),oil palm medium(OPM)
and Woody Plant Medium (WPM). MMS was found to be suitable for the explant of C. renda whereas OPM and WPM were
found to be unsuitable. |
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