Lipid signaling pathways genes expression level in HEPG2 cells supplemented with exogenous lipid and edible bird nest (EBN) extract
“Caviar of the East” or better known as Edible-Bird Nest (EBN) is a highly valuable product composed from solidified salivary secretion of a few insectivorous swiftlet species. Swiftlets falls under the family Apodidae and the two main genera with the highest commercial values are Aerodramus (echola...
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| Format: | Project Paper Report |
| Language: | English |
| Published: |
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/83617/ http://psasir.upm.edu.my/id/eprint/83617/1/FPV%202018%2023%20-%20IR.pdf |
| _version_ | 1848859589457477632 |
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| author | Tawai, Darreen |
| author_facet | Tawai, Darreen |
| author_sort | Tawai, Darreen |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | “Caviar of the East” or better known as Edible-Bird Nest (EBN) is a highly valuable product composed from solidified salivary secretion of a few insectivorous swiftlet species. Swiftlets falls under the family Apodidae and the two main genera with the highest commercial values are Aerodramus (echolating swiftlets) and Collocalia (non-echolating swiftlets). The objective of this study is to determine the qualitative genes expression associated in lipid signalling pathways such as Acetyl-CoA acetyltransferase 2 (ACAT2), Diacylglycerol O-acyltransferase 2 (DGAT2), Low density lipoprotein receptor (LDLR), Sterol regulatory element binding transcription factor 2 (SREBP2), 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR), Proprotein Convertase Subtilisin/kexin type 9 (PCSK9) in HepG2 mammalian cell with or without supplementation of EBN extract and exogenous lipid. Raw EBN were manually cleaned, dried in 60°C oven and left overnight. In this project, EBN supernatant were added with acetone using 1:2 ratio and kept in -80°C for an hour. Hep G2 cells were cultured in six wells plates and each wells were treated according to Base Control, Positive Control, Negative Control, Treatment 1, Treatment 2 and Treatment 3. In this study, there are significant changes in term of genes expression with supplementation of EBN extract especially in ACAT2 and LDLR genes. |
| first_indexed | 2025-11-15T12:31:45Z |
| format | Project Paper Report |
| id | upm-83617 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:31:45Z |
| publishDate | 2018 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-836172020-10-06T02:02:50Z http://psasir.upm.edu.my/id/eprint/83617/ Lipid signaling pathways genes expression level in HEPG2 cells supplemented with exogenous lipid and edible bird nest (EBN) extract Tawai, Darreen “Caviar of the East” or better known as Edible-Bird Nest (EBN) is a highly valuable product composed from solidified salivary secretion of a few insectivorous swiftlet species. Swiftlets falls under the family Apodidae and the two main genera with the highest commercial values are Aerodramus (echolating swiftlets) and Collocalia (non-echolating swiftlets). The objective of this study is to determine the qualitative genes expression associated in lipid signalling pathways such as Acetyl-CoA acetyltransferase 2 (ACAT2), Diacylglycerol O-acyltransferase 2 (DGAT2), Low density lipoprotein receptor (LDLR), Sterol regulatory element binding transcription factor 2 (SREBP2), 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR), Proprotein Convertase Subtilisin/kexin type 9 (PCSK9) in HepG2 mammalian cell with or without supplementation of EBN extract and exogenous lipid. Raw EBN were manually cleaned, dried in 60°C oven and left overnight. In this project, EBN supernatant were added with acetone using 1:2 ratio and kept in -80°C for an hour. Hep G2 cells were cultured in six wells plates and each wells were treated according to Base Control, Positive Control, Negative Control, Treatment 1, Treatment 2 and Treatment 3. In this study, there are significant changes in term of genes expression with supplementation of EBN extract especially in ACAT2 and LDLR genes. 2018-03 Project Paper Report NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/83617/1/FPV%202018%2023%20-%20IR.pdf Tawai, Darreen (2018) Lipid signaling pathways genes expression level in HEPG2 cells supplemented with exogenous lipid and edible bird nest (EBN) extract. [Project Paper Report] |
| spellingShingle | Tawai, Darreen Lipid signaling pathways genes expression level in HEPG2 cells supplemented with exogenous lipid and edible bird nest (EBN) extract |
| title | Lipid signaling pathways genes expression level in HEPG2 cells supplemented with exogenous lipid and edible bird nest (EBN) extract |
| title_full | Lipid signaling pathways genes expression level in HEPG2 cells supplemented with exogenous lipid and edible bird nest (EBN) extract |
| title_fullStr | Lipid signaling pathways genes expression level in HEPG2 cells supplemented with exogenous lipid and edible bird nest (EBN) extract |
| title_full_unstemmed | Lipid signaling pathways genes expression level in HEPG2 cells supplemented with exogenous lipid and edible bird nest (EBN) extract |
| title_short | Lipid signaling pathways genes expression level in HEPG2 cells supplemented with exogenous lipid and edible bird nest (EBN) extract |
| title_sort | lipid signaling pathways genes expression level in hepg2 cells supplemented with exogenous lipid and edible bird nest (ebn) extract |
| url | http://psasir.upm.edu.my/id/eprint/83617/ http://psasir.upm.edu.my/id/eprint/83617/1/FPV%202018%2023%20-%20IR.pdf |