Effect of Storage on the Changes in Cathepsin D Activity, Nucleotide Contents, Peptide Profiles and Muscle Ultrastructure of Aristichthys Nobilis, R
Cathepsin D from the muscle of bighead carp (Aristichthys no bilis, R.)was extracted ,purified and partially characterized . The extract ion and purification o f the enzyme was achieved byautolysis of the muscle,acetone precipitation, gelfiltrat ion on Sephadex G100-120 and on ionexchange carbox...
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| Format: | Thesis |
| Language: | English English |
| Published: |
1993
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| Online Access: | http://psasir.upm.edu.my/id/eprint/8357/ http://psasir.upm.edu.my/id/eprint/8357/1/FSMB_1993_1_A.pdf |
| _version_ | 1848840847496314880 |
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| author | Bakar, Jamilah |
| author_facet | Bakar, Jamilah |
| author_sort | Bakar, Jamilah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Cathepsin D from the muscle of bighead carp (Aristichthys
no bilis, R.)was extracted ,purified and partially
characterized . The extract ion and purification o f the enzyme was achieved byautolysis of the muscle,acetone
precipitation, gelfiltrat ion on Sephadex G100-120 and on ionexchange
carboxymethyl cellulose (CMC)column chromatography .
It had a molecular weight(m.w)of 37,500-38,000 dalton (D)
with a pH optimum at 3.2 and temperature optimum of 50°C.
Myofibril was also optimally digested at pH3.2. The purified
enzyme had a single major peptideband on sodiumdodecyl
sulfate polyacrylamide gelelectrophoresis SDS-PAGE )and was
completely inhibited by pepstatin . |
| first_indexed | 2025-11-15T07:33:51Z |
| format | Thesis |
| id | upm-8357 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T07:33:51Z |
| publishDate | 1993 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-83572012-10-24T07:39:55Z http://psasir.upm.edu.my/id/eprint/8357/ Effect of Storage on the Changes in Cathepsin D Activity, Nucleotide Contents, Peptide Profiles and Muscle Ultrastructure of Aristichthys Nobilis, R Bakar, Jamilah Cathepsin D from the muscle of bighead carp (Aristichthys no bilis, R.)was extracted ,purified and partially characterized . The extract ion and purification o f the enzyme was achieved byautolysis of the muscle,acetone precipitation, gelfiltrat ion on Sephadex G100-120 and on ionexchange carboxymethyl cellulose (CMC)column chromatography . It had a molecular weight(m.w)of 37,500-38,000 dalton (D) with a pH optimum at 3.2 and temperature optimum of 50°C. Myofibril was also optimally digested at pH3.2. The purified enzyme had a single major peptideband on sodiumdodecyl sulfate polyacrylamide gelelectrophoresis SDS-PAGE )and was completely inhibited by pepstatin . 1993 Thesis NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/8357/1/FSMB_1993_1_A.pdf Bakar, Jamilah (1993) Effect of Storage on the Changes in Cathepsin D Activity, Nucleotide Contents, Peptide Profiles and Muscle Ultrastructure of Aristichthys Nobilis, R. PhD thesis, Universiti Pertanian Malaysia. Bighead carp Animal extracts Peptides English |
| spellingShingle | Bighead carp Animal extracts Peptides Bakar, Jamilah Effect of Storage on the Changes in Cathepsin D Activity, Nucleotide Contents, Peptide Profiles and Muscle Ultrastructure of Aristichthys Nobilis, R |
| title | Effect of Storage on the Changes in Cathepsin D Activity, Nucleotide Contents, Peptide Profiles and Muscle Ultrastructure of Aristichthys Nobilis, R |
| title_full | Effect of Storage on the Changes in Cathepsin D Activity, Nucleotide Contents, Peptide Profiles and Muscle Ultrastructure of Aristichthys Nobilis, R |
| title_fullStr | Effect of Storage on the Changes in Cathepsin D Activity, Nucleotide Contents, Peptide Profiles and Muscle Ultrastructure of Aristichthys Nobilis, R |
| title_full_unstemmed | Effect of Storage on the Changes in Cathepsin D Activity, Nucleotide Contents, Peptide Profiles and Muscle Ultrastructure of Aristichthys Nobilis, R |
| title_short | Effect of Storage on the Changes in Cathepsin D Activity, Nucleotide Contents, Peptide Profiles and Muscle Ultrastructure of Aristichthys Nobilis, R |
| title_sort | effect of storage on the changes in cathepsin d activity, nucleotide contents, peptide profiles and muscle ultrastructure of aristichthys nobilis, r |
| topic | Bighead carp Animal extracts Peptides |
| url | http://psasir.upm.edu.my/id/eprint/8357/ http://psasir.upm.edu.my/id/eprint/8357/1/FSMB_1993_1_A.pdf |