Effect of Zerumbone-loaded nanostructured lipid carrier on canine mammary gland tumour cell line
Zerumbone (ZER) from the rhizomes of the wild ginger, Zingiber zerumbet (L.) Smith, is a natural dietary lipophilic compound with antitumour, antiinflammatory, antioxidant, antimicrobial, antinociceptive, hepatoprotective and immunomodulatory properties. However, therapeutic application of zerumbone...
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| Format: | Project Paper Report |
| Language: | English |
| Published: |
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/83395/ http://psasir.upm.edu.my/id/eprint/83395/1/FPV%202015%2013%20IR.pdf |
| _version_ | 1848859529871097856 |
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| author | Foong, Jia Ning |
| author_facet | Foong, Jia Ning |
| author_sort | Foong, Jia Ning |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Zerumbone (ZER) from the rhizomes of the wild ginger, Zingiber zerumbet (L.) Smith, is a natural dietary lipophilic compound with antitumour, antiinflammatory, antioxidant, antimicrobial, antinociceptive, hepatoprotective and immunomodulatory properties. However, therapeutic application of zerumbone is plagued by poor water-solubility and subsequent poor absorption, bioavailability and delivery to target tissues. To overcome this limitation, ZER was loaded into nanostructured lipid carrier (NLC) (ZER-NLC). In this study the anticancer effect of ZER-NLC was determined on a canine mammary gland tumour (CMT-stylo) cell line. It is postulated that loading of ZER into NLC does not compromise the anticancer properties ZER. Using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, the proliferation of CMT-stylo cells after treament with 1.7 mM of ZER was at 64.30±9.87, 42.06±9.00, 37.81±10.04% while with 1.8 mM ZER-NLC at 102.77±12.68, 38.42±9.16, 41.13±11.72% after 24, 48 and 72 hr treatment, respectively. The half maximal lethal dose (LD50) for ZER and ZER-NLC at 72 hr was 100 and 90 μM, respectively. The half maximal growth inhibition dose (GI50) for ZER and ZER-NLC after 72 hr treatment was 20 and 25 μM, respectively. The anticancer effect of ZER and ZER-NLC was also visualised using the acridine orange/propidium iodide double staining method. Zerumbone and ZER-NLC induced apoptosis of CMT-stylo cells as shown by the membrane blebbing, nucleus margination and chromatin condensation. This study, for the first time, shows that ZER-NLC is a potentially effective drug delivery system for the treatment of canine mammary gland tumours. The ZER-NLC is innovative, novel, and safe, for cancer therapy. |
| first_indexed | 2025-11-15T12:30:48Z |
| format | Project Paper Report |
| id | upm-83395 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:30:48Z |
| publishDate | 2015 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-833952020-09-17T04:14:45Z http://psasir.upm.edu.my/id/eprint/83395/ Effect of Zerumbone-loaded nanostructured lipid carrier on canine mammary gland tumour cell line Foong, Jia Ning Zerumbone (ZER) from the rhizomes of the wild ginger, Zingiber zerumbet (L.) Smith, is a natural dietary lipophilic compound with antitumour, antiinflammatory, antioxidant, antimicrobial, antinociceptive, hepatoprotective and immunomodulatory properties. However, therapeutic application of zerumbone is plagued by poor water-solubility and subsequent poor absorption, bioavailability and delivery to target tissues. To overcome this limitation, ZER was loaded into nanostructured lipid carrier (NLC) (ZER-NLC). In this study the anticancer effect of ZER-NLC was determined on a canine mammary gland tumour (CMT-stylo) cell line. It is postulated that loading of ZER into NLC does not compromise the anticancer properties ZER. Using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, the proliferation of CMT-stylo cells after treament with 1.7 mM of ZER was at 64.30±9.87, 42.06±9.00, 37.81±10.04% while with 1.8 mM ZER-NLC at 102.77±12.68, 38.42±9.16, 41.13±11.72% after 24, 48 and 72 hr treatment, respectively. The half maximal lethal dose (LD50) for ZER and ZER-NLC at 72 hr was 100 and 90 μM, respectively. The half maximal growth inhibition dose (GI50) for ZER and ZER-NLC after 72 hr treatment was 20 and 25 μM, respectively. The anticancer effect of ZER and ZER-NLC was also visualised using the acridine orange/propidium iodide double staining method. Zerumbone and ZER-NLC induced apoptosis of CMT-stylo cells as shown by the membrane blebbing, nucleus margination and chromatin condensation. This study, for the first time, shows that ZER-NLC is a potentially effective drug delivery system for the treatment of canine mammary gland tumours. The ZER-NLC is innovative, novel, and safe, for cancer therapy. 2015-02 Project Paper Report NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/83395/1/FPV%202015%2013%20IR.pdf Foong, Jia Ning (2015) Effect of Zerumbone-loaded nanostructured lipid carrier on canine mammary gland tumour cell line. [Project Paper Report] |
| spellingShingle | Foong, Jia Ning Effect of Zerumbone-loaded nanostructured lipid carrier on canine mammary gland tumour cell line |
| title | Effect of Zerumbone-loaded nanostructured lipid carrier on canine mammary gland tumour cell line |
| title_full | Effect of Zerumbone-loaded nanostructured lipid carrier on canine mammary gland tumour cell line |
| title_fullStr | Effect of Zerumbone-loaded nanostructured lipid carrier on canine mammary gland tumour cell line |
| title_full_unstemmed | Effect of Zerumbone-loaded nanostructured lipid carrier on canine mammary gland tumour cell line |
| title_short | Effect of Zerumbone-loaded nanostructured lipid carrier on canine mammary gland tumour cell line |
| title_sort | effect of zerumbone-loaded nanostructured lipid carrier on canine mammary gland tumour cell line |
| url | http://psasir.upm.edu.my/id/eprint/83395/ http://psasir.upm.edu.my/id/eprint/83395/1/FPV%202015%2013%20IR.pdf |