Effects of antifungal treatment on the embryonic development, hatching, and larval survival of Clarias gariepinus (Burchell, 1822)

A study on the effect of different antifungal agents on the embryonic development, hatching and survival of African catfish, Clarias gariepinus was carried out from April to August 2013. Eggs and sperms of C. gariepinus was produced through induced breeding technique using ovaprim hormone. Eggs were...

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Main Author: Mun, Kelvin Leong Wai
Format: Project Paper Report
Language:English
Published: 2013
Online Access:http://psasir.upm.edu.my/id/eprint/82795/
http://psasir.upm.edu.my/id/eprint/82795/1/FP%202013%2090%20-%20IR.pdf
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author Mun, Kelvin Leong Wai
author_facet Mun, Kelvin Leong Wai
author_sort Mun, Kelvin Leong Wai
building UPM Institutional Repository
collection Online Access
description A study on the effect of different antifungal agents on the embryonic development, hatching and survival of African catfish, Clarias gariepinus was carried out from April to August 2013. Eggs and sperms of C. gariepinus was produced through induced breeding technique using ovaprim hormone. Eggs were fertilized using dry method. Newly fertilized eggs were treated for 24 hours with different dosages of formalin, chamomile extract and methylene blue at incubation temperature of 27±1˚C. Embryonic development was monitored at hourly intervals for 24 hours. Formalin treatment at 15, 30 and 45 ppm showed the highest percentage of hatching (p<0.05), of 59, 57, and 58% respectively as compared to other treatments. However, this treatment produced the highest numbers of deformed larvae (p<0.05). In contrast, treatment using chamomile produced the lowest numbers of deformed larvae (p<0.05). This study showed that formalin is the most effective anti-fungal treatment, evident with the highest percentage of hatching. However consideration on safety and environmental issues need to be given attention. Second best alternative is using chamomile extract as antifungal for the eggs of C. gariepinus. Fungal growth was observed as early as 15 hours after fertilization. Overall, all the anti-fungal treatments used in this study do not affect the development C. gariepinus embryos.
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spelling upm-827952020-07-14T07:21:54Z http://psasir.upm.edu.my/id/eprint/82795/ Effects of antifungal treatment on the embryonic development, hatching, and larval survival of Clarias gariepinus (Burchell, 1822) Mun, Kelvin Leong Wai A study on the effect of different antifungal agents on the embryonic development, hatching and survival of African catfish, Clarias gariepinus was carried out from April to August 2013. Eggs and sperms of C. gariepinus was produced through induced breeding technique using ovaprim hormone. Eggs were fertilized using dry method. Newly fertilized eggs were treated for 24 hours with different dosages of formalin, chamomile extract and methylene blue at incubation temperature of 27±1˚C. Embryonic development was monitored at hourly intervals for 24 hours. Formalin treatment at 15, 30 and 45 ppm showed the highest percentage of hatching (p<0.05), of 59, 57, and 58% respectively as compared to other treatments. However, this treatment produced the highest numbers of deformed larvae (p<0.05). In contrast, treatment using chamomile produced the lowest numbers of deformed larvae (p<0.05). This study showed that formalin is the most effective anti-fungal treatment, evident with the highest percentage of hatching. However consideration on safety and environmental issues need to be given attention. Second best alternative is using chamomile extract as antifungal for the eggs of C. gariepinus. Fungal growth was observed as early as 15 hours after fertilization. Overall, all the anti-fungal treatments used in this study do not affect the development C. gariepinus embryos. 2013 Project Paper Report NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/82795/1/FP%202013%2090%20-%20IR.pdf Mun, Kelvin Leong Wai (2013) Effects of antifungal treatment on the embryonic development, hatching, and larval survival of Clarias gariepinus (Burchell, 1822). [Project Paper Report]
spellingShingle Mun, Kelvin Leong Wai
Effects of antifungal treatment on the embryonic development, hatching, and larval survival of Clarias gariepinus (Burchell, 1822)
title Effects of antifungal treatment on the embryonic development, hatching, and larval survival of Clarias gariepinus (Burchell, 1822)
title_full Effects of antifungal treatment on the embryonic development, hatching, and larval survival of Clarias gariepinus (Burchell, 1822)
title_fullStr Effects of antifungal treatment on the embryonic development, hatching, and larval survival of Clarias gariepinus (Burchell, 1822)
title_full_unstemmed Effects of antifungal treatment on the embryonic development, hatching, and larval survival of Clarias gariepinus (Burchell, 1822)
title_short Effects of antifungal treatment on the embryonic development, hatching, and larval survival of Clarias gariepinus (Burchell, 1822)
title_sort effects of antifungal treatment on the embryonic development, hatching, and larval survival of clarias gariepinus (burchell, 1822)
url http://psasir.upm.edu.my/id/eprint/82795/
http://psasir.upm.edu.my/id/eprint/82795/1/FP%202013%2090%20-%20IR.pdf