Voltammetric determination of human papillomavirus 16 DNA by using interdigitated electrodes modified with titanium dioxide nanoparticles
A gene sensor for rapid detection of the Human Papillomavirus 16 (HPV 16) which is associated with the appearance of cervical cancer was developed. The assay is based on voltammetric determination of HPV 16 DNA by using interdigitated electrodes modified with titanium dioxide nanoparticles. Titanium...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Springer
2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/82736/ http://psasir.upm.edu.my/id/eprint/82736/1/Voltammetric%20determination%20of%20human%20papillomavirus%2016%20DNA%20by%20using%20interdigitated%20electrodes%20modified%20with%20titanium%20dioxide%20nanoparticles.pdf |
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| author | Parmin, Nor Azizah Hashim, Uda Gopinath, Subash C. B. Nadzirah, Sharipah Rejali, Zulida Mohd Jamil, Amilia Afzan Aiman Uda, Muhammad Nur Hong, V. C. Rajapaksha, Asanka |
| author_facet | Parmin, Nor Azizah Hashim, Uda Gopinath, Subash C. B. Nadzirah, Sharipah Rejali, Zulida Mohd Jamil, Amilia Afzan Aiman Uda, Muhammad Nur Hong, V. C. Rajapaksha, Asanka |
| author_sort | Parmin, Nor Azizah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | A gene sensor for rapid detection of the Human Papillomavirus 16 (HPV 16) which is associated with the appearance of cervical cancer was developed. The assay is based on voltammetric determination of HPV 16 DNA by using interdigitated electrodes modified with titanium dioxide nanoparticles. Titanium dioxide nanoparticles (NPs) were used to modify a semiconductor-based interdigitated electrode (IDE). The surface of the NPs was then functionalized with a commercial 24-mer oligomer DNA probe for HPV 16 that was modified at the 5′ end with a carboxyl group. If the probe interacts with the HPV 16 ssDNA, the current, best measured at a working voltage of 1.0 V, increases. The gene sensor has has a ∼ 0.1 fM limit of detection which is comparable to other sensors. The dielectric voltammetry analysis was carried out from 0 V to 1 V. The electrochemical sensitivity of the IDE is 2.5 × 10⁻⁵ μA·μM⁻¹·cm⁻². Graphical abstractSchematic of an interdigitated electrode (IDE) modified with titanium dioxide nanoparticles for voltammetric determination of HPV 16 DNA by using an appropriate DNA probe. |
| first_indexed | 2025-11-15T12:27:48Z |
| format | Article |
| id | upm-82736 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:27:48Z |
| publishDate | 2019 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-827362020-10-16T06:12:59Z http://psasir.upm.edu.my/id/eprint/82736/ Voltammetric determination of human papillomavirus 16 DNA by using interdigitated electrodes modified with titanium dioxide nanoparticles Parmin, Nor Azizah Hashim, Uda Gopinath, Subash C. B. Nadzirah, Sharipah Rejali, Zulida Mohd Jamil, Amilia Afzan Aiman Uda, Muhammad Nur Hong, V. C. Rajapaksha, Asanka A gene sensor for rapid detection of the Human Papillomavirus 16 (HPV 16) which is associated with the appearance of cervical cancer was developed. The assay is based on voltammetric determination of HPV 16 DNA by using interdigitated electrodes modified with titanium dioxide nanoparticles. Titanium dioxide nanoparticles (NPs) were used to modify a semiconductor-based interdigitated electrode (IDE). The surface of the NPs was then functionalized with a commercial 24-mer oligomer DNA probe for HPV 16 that was modified at the 5′ end with a carboxyl group. If the probe interacts with the HPV 16 ssDNA, the current, best measured at a working voltage of 1.0 V, increases. The gene sensor has has a ∼ 0.1 fM limit of detection which is comparable to other sensors. The dielectric voltammetry analysis was carried out from 0 V to 1 V. The electrochemical sensitivity of the IDE is 2.5 × 10⁻⁵ μA·μM⁻¹·cm⁻². Graphical abstractSchematic of an interdigitated electrode (IDE) modified with titanium dioxide nanoparticles for voltammetric determination of HPV 16 DNA by using an appropriate DNA probe. Springer 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/82736/1/Voltammetric%20determination%20of%20human%20papillomavirus%2016%20DNA%20by%20using%20interdigitated%20electrodes%20modified%20with%20titanium%20dioxide%20nanoparticles.pdf Parmin, Nor Azizah and Hashim, Uda and Gopinath, Subash C. B. and Nadzirah, Sharipah and Rejali, Zulida and Mohd Jamil, Amilia Afzan and Aiman Uda, Muhammad Nur and Hong, V. C. and Rajapaksha, Asanka (2019) Voltammetric determination of human papillomavirus 16 DNA by using interdigitated electrodes modified with titanium dioxide nanoparticles. Microchimica Acta, 186 (336). pp. 1-9. ISSN 0026-3672 https://link.springer.com/article/10.1007/s00604-019-3445-2 10.1007/s00604-019-3445-2 |
| spellingShingle | Parmin, Nor Azizah Hashim, Uda Gopinath, Subash C. B. Nadzirah, Sharipah Rejali, Zulida Mohd Jamil, Amilia Afzan Aiman Uda, Muhammad Nur Hong, V. C. Rajapaksha, Asanka Voltammetric determination of human papillomavirus 16 DNA by using interdigitated electrodes modified with titanium dioxide nanoparticles |
| title | Voltammetric determination of human papillomavirus 16 DNA by using interdigitated electrodes modified with titanium dioxide nanoparticles |
| title_full | Voltammetric determination of human papillomavirus 16 DNA by using interdigitated electrodes modified with titanium dioxide nanoparticles |
| title_fullStr | Voltammetric determination of human papillomavirus 16 DNA by using interdigitated electrodes modified with titanium dioxide nanoparticles |
| title_full_unstemmed | Voltammetric determination of human papillomavirus 16 DNA by using interdigitated electrodes modified with titanium dioxide nanoparticles |
| title_short | Voltammetric determination of human papillomavirus 16 DNA by using interdigitated electrodes modified with titanium dioxide nanoparticles |
| title_sort | voltammetric determination of human papillomavirus 16 dna by using interdigitated electrodes modified with titanium dioxide nanoparticles |
| url | http://psasir.upm.edu.my/id/eprint/82736/ http://psasir.upm.edu.my/id/eprint/82736/ http://psasir.upm.edu.my/id/eprint/82736/ http://psasir.upm.edu.my/id/eprint/82736/1/Voltammetric%20determination%20of%20human%20papillomavirus%2016%20DNA%20by%20using%20interdigitated%20electrodes%20modified%20with%20titanium%20dioxide%20nanoparticles.pdf |