Vibrio harveyi protease deletion mutant as a live attenuated vaccine candidate against vibriosis and transcriptome profiling following vaccination for Epinephelus fuscoguttatus
Grouper aquaculture industries have a high risk of being inflicted by bacterial diseases such as vibriosis. Various types of vaccines for vibriosis have been studied throughout the years, yet the potential of live attenuated vaccines remains unsubstantial. Correspondingly, this study attempts to dev...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Springer
2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/82719/ http://psasir.upm.edu.my/id/eprint/82719/1/Vibrio%20harveyi%20.pdf |
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| author | Mohd Aris, Aslizah Saad, Mohd Zamri Mohd Daud, Hassan Yusof, Mohd. Termizi Md Yasin, Ina Salwany |
| author_facet | Mohd Aris, Aslizah Saad, Mohd Zamri Mohd Daud, Hassan Yusof, Mohd. Termizi Md Yasin, Ina Salwany |
| author_sort | Mohd Aris, Aslizah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Grouper aquaculture industries have a high risk of being inflicted by bacterial diseases such as vibriosis. Various types of vaccines for vibriosis have been studied throughout the years, yet the potential of live attenuated vaccines remains unsubstantial. Correspondingly, this study attempts to develop a Vibrio harveyi protease deletion mutant into a live attenuated vaccine candidate against vibriosis for Epinephelus fuscoguttatus. Site-directed mutagenesis (SDM) and allelic exchange replacement techniques are employed to synthesize genetically attenuated V. harveyi strain MVh-vhs. The evaluation on safety levels showed that MVh-vhs strain is safe when tested on E. fuscoguttatus. A 100% survival rate with no sign of vibriosis is indicated in fish challenged with the attenuated strain. In contrast, fish challenged with the parental strain showed obvious clinical signs of vibriosis. The median lethal dosage (LD50) of fish challenged with the parental strain is found at 10⁶ CFU/fish. A single dose IP administration of the attenuated strain at 10⁵ CFU/fish following a bacterial challenge at dose 10⁸ CFU/fish is done 4 weeks post vaccination. The vaccinated fish show 52% relative percentage survival (RPS). The transcriptomic profiling following vaccination evoked the regulation of autophagosome pathway and the coagulation and complement cascade pathways as well as antigen processing and presentation pathways. As a conclusion, the V. harveyi attenuated strain MVh-vhs has significant potential to be applied as a live vaccine candidate against vibriosis for E. fuscoguttatus. |
| first_indexed | 2025-11-15T12:27:44Z |
| format | Article |
| id | upm-82719 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:27:44Z |
| publishDate | 2019 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-827192021-06-10T06:26:31Z http://psasir.upm.edu.my/id/eprint/82719/ Vibrio harveyi protease deletion mutant as a live attenuated vaccine candidate against vibriosis and transcriptome profiling following vaccination for Epinephelus fuscoguttatus Mohd Aris, Aslizah Saad, Mohd Zamri Mohd Daud, Hassan Yusof, Mohd. Termizi Md Yasin, Ina Salwany Grouper aquaculture industries have a high risk of being inflicted by bacterial diseases such as vibriosis. Various types of vaccines for vibriosis have been studied throughout the years, yet the potential of live attenuated vaccines remains unsubstantial. Correspondingly, this study attempts to develop a Vibrio harveyi protease deletion mutant into a live attenuated vaccine candidate against vibriosis for Epinephelus fuscoguttatus. Site-directed mutagenesis (SDM) and allelic exchange replacement techniques are employed to synthesize genetically attenuated V. harveyi strain MVh-vhs. The evaluation on safety levels showed that MVh-vhs strain is safe when tested on E. fuscoguttatus. A 100% survival rate with no sign of vibriosis is indicated in fish challenged with the attenuated strain. In contrast, fish challenged with the parental strain showed obvious clinical signs of vibriosis. The median lethal dosage (LD50) of fish challenged with the parental strain is found at 10⁶ CFU/fish. A single dose IP administration of the attenuated strain at 10⁵ CFU/fish following a bacterial challenge at dose 10⁸ CFU/fish is done 4 weeks post vaccination. The vaccinated fish show 52% relative percentage survival (RPS). The transcriptomic profiling following vaccination evoked the regulation of autophagosome pathway and the coagulation and complement cascade pathways as well as antigen processing and presentation pathways. As a conclusion, the V. harveyi attenuated strain MVh-vhs has significant potential to be applied as a live vaccine candidate against vibriosis for E. fuscoguttatus. Springer 2019-02 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/82719/1/Vibrio%20harveyi%20.pdf Mohd Aris, Aslizah and Saad, Mohd Zamri and Mohd Daud, Hassan and Yusof, Mohd. Termizi and Md Yasin, Ina Salwany (2019) Vibrio harveyi protease deletion mutant as a live attenuated vaccine candidate against vibriosis and transcriptome profiling following vaccination for Epinephelus fuscoguttatus. Aquaculture International, 27 (1). pp. 125-140. ISSN 0967-6120; ESSN 1573-143X https://link.springer.com/article/10.1007/s10499-018-0311-x 10.1007/s10499-018-0311-x |
| spellingShingle | Mohd Aris, Aslizah Saad, Mohd Zamri Mohd Daud, Hassan Yusof, Mohd. Termizi Md Yasin, Ina Salwany Vibrio harveyi protease deletion mutant as a live attenuated vaccine candidate against vibriosis and transcriptome profiling following vaccination for Epinephelus fuscoguttatus |
| title | Vibrio harveyi protease deletion mutant as a live attenuated vaccine candidate against vibriosis and transcriptome profiling following vaccination for Epinephelus fuscoguttatus |
| title_full | Vibrio harveyi protease deletion mutant as a live attenuated vaccine candidate against vibriosis and transcriptome profiling following vaccination for Epinephelus fuscoguttatus |
| title_fullStr | Vibrio harveyi protease deletion mutant as a live attenuated vaccine candidate against vibriosis and transcriptome profiling following vaccination for Epinephelus fuscoguttatus |
| title_full_unstemmed | Vibrio harveyi protease deletion mutant as a live attenuated vaccine candidate against vibriosis and transcriptome profiling following vaccination for Epinephelus fuscoguttatus |
| title_short | Vibrio harveyi protease deletion mutant as a live attenuated vaccine candidate against vibriosis and transcriptome profiling following vaccination for Epinephelus fuscoguttatus |
| title_sort | vibrio harveyi protease deletion mutant as a live attenuated vaccine candidate against vibriosis and transcriptome profiling following vaccination for epinephelus fuscoguttatus |
| url | http://psasir.upm.edu.my/id/eprint/82719/ http://psasir.upm.edu.my/id/eprint/82719/ http://psasir.upm.edu.my/id/eprint/82719/ http://psasir.upm.edu.my/id/eprint/82719/1/Vibrio%20harveyi%20.pdf |